Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach
Abstract Background The use of contact lenses has increased in recent years as has the incidence of Dry Eye Syndrome, partly due to their use. Artificial tears are the most common treatment option. Since these changes can facilitate Acanthamoeba infection, the present study has been designed to eval...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-01-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-018-2639-5 |
| _version_ | 1817995869484482560 |
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| author | Angela Magnet Thiago Santos Gomes Carmen Pardinas Natalia Garcia de Blas Cruz Sadaba Eugenia Carrillo Fernando Izquierdo José Manuel Benítez del Castillo Carolina Hurtado Carmen del Aguila Soledad Fenoy |
| author_facet | Angela Magnet Thiago Santos Gomes Carmen Pardinas Natalia Garcia de Blas Cruz Sadaba Eugenia Carrillo Fernando Izquierdo José Manuel Benítez del Castillo Carolina Hurtado Carmen del Aguila Soledad Fenoy |
| author_sort | Angela Magnet |
| collection | DOAJ |
| description | Abstract Background The use of contact lenses has increased in recent years as has the incidence of Dry Eye Syndrome, partly due to their use. Artificial tears are the most common treatment option. Since these changes can facilitate Acanthamoeba infection, the present study has been designed to evaluate the effect of three artificial tears treatments in the viability of Acanthamoeba genotype T4 trophozoites. Optava Fusion™, Oculotect®, and Artelac® Splash were selected due to their formulation. Methods Viability was assessed using two staining methods, Trypan Blue stain and CTC stain at different time intervals (2, 4, 6, 8 and 24 h). Trypan Blue viability was obtained by manual count with light microscopy while the CTC stain was determined using flow cytometry. Results Trypan Blue staining results demonstrated a decrease in viability for Optava Fusion™ and Artelac® Splash during the first 4 h of incubation. After, this effect seems to lose strength. In the case of Oculotect®, complete cell death was observed after 2 h. Using flow cytometry analysis, Optava Fusion™ and Oculotect® exhibited the same effect observed with Trypan Blue staining. However, Artelac® Splash revealed decreasing cell respiratory activity after four hours, with no damage to the cell membrane. Conclusions The present study uses, for the first time, CTC stain analyzed by flow cytometry to establish Acanthamoeba viability demonstrating its usefulness and complementarity with the traditional stain, Trypan Blue. Artelac® Splash, with no preservatives, and Optava Fusion TM, with Purite®, have not shown any useful amoebicidal activity. On the contrary, promising results presented by Ocultect®, with BAK, open up a new possibility for Acanthamoeba keratitis prophylaxis and treatment although in vivo studies should be carried out. |
| first_indexed | 2024-04-14T02:13:39Z |
| format | Article |
| id | doaj.art-d1eb603782ca4dd5b81ae2b8bf3c639c |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-04-14T02:13:39Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-d1eb603782ca4dd5b81ae2b8bf3c639c2022-12-22T02:18:20ZengBMCParasites & Vectors1756-33052018-01-011111710.1186/s13071-018-2639-5Can artificial tears prevent Acanthamoeba keratitis? An in vitro approachAngela Magnet0Thiago Santos Gomes1Carmen Pardinas2Natalia Garcia de Blas3Cruz Sadaba4Eugenia Carrillo5Fernando Izquierdo6José Manuel Benítez del Castillo7Carolina Hurtado8Carmen del Aguila9Soledad Fenoy10Facultad de Farmacia, Universidad San Pablo CEU, CEU UniversitiesFacultad de Farmacia, Universidad San Pablo CEU, CEU UniversitiesFacultad de Farmacia, Universidad San Pablo CEU, CEU UniversitiesFacultad de Farmacia, Universidad San Pablo CEU, CEU UniversitiesFacultad de Medicina, Universidad San Pablo CEU, CEU UniversitiesInstituto de Salud Carlos III, Centro Nacional de Microbiología, WHO Collaborating Centre for LeishmaniasisFacultad de Farmacia, Universidad San Pablo CEU, CEU UniversitiesServicio de Oftalmología, Hospital Clínico San Carlos, Universidad ComplutenseFacultad de Farmacia, Universidad San Pablo CEU, CEU UniversitiesFacultad de Farmacia, Universidad San Pablo CEU, CEU UniversitiesFacultad de Farmacia, Universidad San Pablo CEU, CEU UniversitiesAbstract Background The use of contact lenses has increased in recent years as has the incidence of Dry Eye Syndrome, partly due to their use. Artificial tears are the most common treatment option. Since these changes can facilitate Acanthamoeba infection, the present study has been designed to evaluate the effect of three artificial tears treatments in the viability of Acanthamoeba genotype T4 trophozoites. Optava Fusion™, Oculotect®, and Artelac® Splash were selected due to their formulation. Methods Viability was assessed using two staining methods, Trypan Blue stain and CTC stain at different time intervals (2, 4, 6, 8 and 24 h). Trypan Blue viability was obtained by manual count with light microscopy while the CTC stain was determined using flow cytometry. Results Trypan Blue staining results demonstrated a decrease in viability for Optava Fusion™ and Artelac® Splash during the first 4 h of incubation. After, this effect seems to lose strength. In the case of Oculotect®, complete cell death was observed after 2 h. Using flow cytometry analysis, Optava Fusion™ and Oculotect® exhibited the same effect observed with Trypan Blue staining. However, Artelac® Splash revealed decreasing cell respiratory activity after four hours, with no damage to the cell membrane. Conclusions The present study uses, for the first time, CTC stain analyzed by flow cytometry to establish Acanthamoeba viability demonstrating its usefulness and complementarity with the traditional stain, Trypan Blue. Artelac® Splash, with no preservatives, and Optava Fusion TM, with Purite®, have not shown any useful amoebicidal activity. On the contrary, promising results presented by Ocultect®, with BAK, open up a new possibility for Acanthamoeba keratitis prophylaxis and treatment although in vivo studies should be carried out.http://link.springer.com/article/10.1186/s13071-018-2639-5AcanthamoebaProphylaxisArtificial tearsPreservativesAmoebicidal effect |
| spellingShingle | Angela Magnet Thiago Santos Gomes Carmen Pardinas Natalia Garcia de Blas Cruz Sadaba Eugenia Carrillo Fernando Izquierdo José Manuel Benítez del Castillo Carolina Hurtado Carmen del Aguila Soledad Fenoy Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach Parasites & Vectors Acanthamoeba Prophylaxis Artificial tears Preservatives Amoebicidal effect |
| title | Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach |
| title_full | Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach |
| title_fullStr | Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach |
| title_full_unstemmed | Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach |
| title_short | Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach |
| title_sort | can artificial tears prevent acanthamoeba keratitis an in vitro approach |
| topic | Acanthamoeba Prophylaxis Artificial tears Preservatives Amoebicidal effect |
| url | http://link.springer.com/article/10.1186/s13071-018-2639-5 |
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