The Impact of Storage Temperature and Time on Ergot Alkaloid Concentrations
Ergot sclerotia produce toxic secondary metabolites, ergot alkaloids, that infect cereal crops and grasses. Ergot alkaloids have two isomeric configurations: the C-8-<i>R</i>-isomer (<i>R</i>-epimer), and the C-8-<i>S</i>-isomer (<i>S</i>-epimer). Ergo...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/15/8/497 |
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| author | Jensen E. Cherewyk Taylor J. Grusie-Ogilvie Sarah E. Parker Barry R. Blakley Ahmad N. Al-Dissi |
| author_facet | Jensen E. Cherewyk Taylor J. Grusie-Ogilvie Sarah E. Parker Barry R. Blakley Ahmad N. Al-Dissi |
| author_sort | Jensen E. Cherewyk |
| collection | DOAJ |
| description | Ergot sclerotia produce toxic secondary metabolites, ergot alkaloids, that infect cereal crops and grasses. Ergot alkaloids have two isomeric configurations: the C-8-<i>R</i>-isomer (<i>R</i>-epimer), and the C-8-<i>S</i>-isomer (<i>S</i>-epimer). Ergot contaminated matrices, such as cereal grains or grasses, may be stored for extended periods at various temperatures before being analyzed, utilized, or consumed. This study assessed the concentration of six common ergot alkaloids in both configurations found in naturally contaminated wheat over time (one, two, and four months) at different temperatures (room temperature, +4 °C, and −20 °C) using ultra-high-performance liquid chromatography–tandem mass spectrometry. The data indicate that the total ergot concentration within a natural contaminated sample varies over time at room temperature, +4 °C, and −20 °C. The total ergot concentration increased until month two, and decreased at month four, independent of temperature (<i>p</i> < 0.05). The total <i>R</i>-epimer concentration appeared to be less stable over time than the total <i>S</i>-epimer concentration. The changes in the total <i>R</i> and total <i>S</i>-epimer concentrations may have been caused by changes in the ergocristine and ergocristinine concentrations, respectively. Time and temperature should be considered when storing potentially contaminated matrices in a laboratory or practical agriculture situations. Quantification of ergot contaminated matrices should occur prior to their use to ensure the most reliable estimates of the concentration of ergot. |
| first_indexed | 2024-03-10T23:32:03Z |
| format | Article |
| id | doaj.art-d1ed61182c944d0fa83017a1e1f96adb |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T23:32:03Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-d1ed61182c944d0fa83017a1e1f96adb2023-11-19T03:15:27ZengMDPI AGToxins2072-66512023-08-0115849710.3390/toxins15080497The Impact of Storage Temperature and Time on Ergot Alkaloid ConcentrationsJensen E. Cherewyk0Taylor J. Grusie-Ogilvie1Sarah E. Parker2Barry R. Blakley3Ahmad N. Al-Dissi4Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, CanadaPrairie Diagnostic Services (PDS), Saskatoon, SK S7N 5B4, CanadaCentre for Applied Epidemiology, Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, CanadaDepartment of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, CanadaDepartment of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, CanadaErgot sclerotia produce toxic secondary metabolites, ergot alkaloids, that infect cereal crops and grasses. Ergot alkaloids have two isomeric configurations: the C-8-<i>R</i>-isomer (<i>R</i>-epimer), and the C-8-<i>S</i>-isomer (<i>S</i>-epimer). Ergot contaminated matrices, such as cereal grains or grasses, may be stored for extended periods at various temperatures before being analyzed, utilized, or consumed. This study assessed the concentration of six common ergot alkaloids in both configurations found in naturally contaminated wheat over time (one, two, and four months) at different temperatures (room temperature, +4 °C, and −20 °C) using ultra-high-performance liquid chromatography–tandem mass spectrometry. The data indicate that the total ergot concentration within a natural contaminated sample varies over time at room temperature, +4 °C, and −20 °C. The total ergot concentration increased until month two, and decreased at month four, independent of temperature (<i>p</i> < 0.05). The total <i>R</i>-epimer concentration appeared to be less stable over time than the total <i>S</i>-epimer concentration. The changes in the total <i>R</i> and total <i>S</i>-epimer concentrations may have been caused by changes in the ergocristine and ergocristinine concentrations, respectively. Time and temperature should be considered when storing potentially contaminated matrices in a laboratory or practical agriculture situations. Quantification of ergot contaminated matrices should occur prior to their use to ensure the most reliable estimates of the concentration of ergot.https://www.mdpi.com/2072-6651/15/8/497contaminationgrainepimer |
| spellingShingle | Jensen E. Cherewyk Taylor J. Grusie-Ogilvie Sarah E. Parker Barry R. Blakley Ahmad N. Al-Dissi The Impact of Storage Temperature and Time on Ergot Alkaloid Concentrations Toxins contamination grain epimer |
| title | The Impact of Storage Temperature and Time on Ergot Alkaloid Concentrations |
| title_full | The Impact of Storage Temperature and Time on Ergot Alkaloid Concentrations |
| title_fullStr | The Impact of Storage Temperature and Time on Ergot Alkaloid Concentrations |
| title_full_unstemmed | The Impact of Storage Temperature and Time on Ergot Alkaloid Concentrations |
| title_short | The Impact of Storage Temperature and Time on Ergot Alkaloid Concentrations |
| title_sort | impact of storage temperature and time on ergot alkaloid concentrations |
| topic | contamination grain epimer |
| url | https://www.mdpi.com/2072-6651/15/8/497 |
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