Production of indole acetic acid by Kocuria rosea VB1 and Arthrobacter luteolus VB2 under the influence of L-tryptophan and maize root exudates
Phytohormones play a very important role in enhancing plant growth by direct or indirect mechanisms involving plant-microbe interactions. Indole acetic acid (IAA) is one of the key phytohormones that directly enhance plant development. Kocuria rosea VB1 (GenBank ID: KY608093.1) and Arthrobacter lute...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2019-03-01
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| Series: | BioTechnologia |
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| Online Access: | https://www.termedia.pl/Production-of-indole-acetic-acid-by-Kocuria-rosea-VB1-and-Arthrobacter-luteolus-VB2-under-the-influence-of-L-tryptophan-and-maize-root-exudates,85,35923,1,1.html |
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| author | Arun Karnwal |
| author_facet | Arun Karnwal |
| author_sort | Arun Karnwal |
| collection | DOAJ |
| description | Phytohormones play a very important role in enhancing plant growth by direct or indirect mechanisms involving plant-microbe interactions. Indole acetic acid (IAA) is one of the key phytohormones that directly enhance plant development. Kocuria rosea VB1 (GenBank ID: KY608093.1) and Arthrobacter luteolus VB2 (GenBank ID: KY608094.1) from polluted industrial water samples were characterized through a biochemical assay, 16S rDNA sequencing, and for promoting plant growth abilities. IAA production by VB1 and VB2 was tested in pure culture conditions supplemented with various L-tryptophan (Trp) concentrations (0, 50, 100, 200, and 500 μg • ml -1 ). A significant difference in indole production by VB2 and VB1 inoculant at various L-Trp concentrations has been observed. VB1 has been reported to produce increased amounts of indole from 0.33 μg • ml -1 to 18.16 μg • ml -1 , while the increase in indole production was from 0.63 μg • ml -1 to 9.22 μg • ml -1 for VB2 for various L-Trp concentrations. The VB1 strain produced 85.07 ng • ml -1 and 123.7 ng • ml -1 IAA, whereas the VB2 strain produced 70.3 ng • ml -1 and 78.4 ng • ml -1 IAA, respectively at 200 and 500 μg • ml -1 Trp concentrations. The growth pouch experiments with maize root exudates also showed a positive effect for both bacterial inoculants tested on IAA biosynthesis in comparison to non-inoculated seeds. Inoculation of maize seeds with VB2 and VB1 bacteria gave a significantly higher level of IAA production in comparison to non-inoculated seeds. Current study outcomes show the beneficial aspects of plant growth regulators produced by free-living bacteria which could play a significant role in plant growth promotion. |
| first_indexed | 2024-12-10T14:48:32Z |
| format | Article |
| id | doaj.art-d1ed827973764f4fa52a3a573def00ef |
| institution | Directory Open Access Journal |
| issn | 0860-7796 2353-9461 |
| language | English |
| last_indexed | 2024-12-10T14:48:32Z |
| publishDate | 2019-03-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | BioTechnologia |
| spelling | doaj.art-d1ed827973764f4fa52a3a573def00ef2022-12-22T01:44:30ZengTermedia Publishing HouseBioTechnologia0860-77962353-94612019-03-011001293510.5114/bta.2019.8320935923Production of indole acetic acid by Kocuria rosea VB1 and Arthrobacter luteolus VB2 under the influence of L-tryptophan and maize root exudatesArun KarnwalPhytohormones play a very important role in enhancing plant growth by direct or indirect mechanisms involving plant-microbe interactions. Indole acetic acid (IAA) is one of the key phytohormones that directly enhance plant development. Kocuria rosea VB1 (GenBank ID: KY608093.1) and Arthrobacter luteolus VB2 (GenBank ID: KY608094.1) from polluted industrial water samples were characterized through a biochemical assay, 16S rDNA sequencing, and for promoting plant growth abilities. IAA production by VB1 and VB2 was tested in pure culture conditions supplemented with various L-tryptophan (Trp) concentrations (0, 50, 100, 200, and 500 μg • ml -1 ). A significant difference in indole production by VB2 and VB1 inoculant at various L-Trp concentrations has been observed. VB1 has been reported to produce increased amounts of indole from 0.33 μg • ml -1 to 18.16 μg • ml -1 , while the increase in indole production was from 0.63 μg • ml -1 to 9.22 μg • ml -1 for VB2 for various L-Trp concentrations. The VB1 strain produced 85.07 ng • ml -1 and 123.7 ng • ml -1 IAA, whereas the VB2 strain produced 70.3 ng • ml -1 and 78.4 ng • ml -1 IAA, respectively at 200 and 500 μg • ml -1 Trp concentrations. The growth pouch experiments with maize root exudates also showed a positive effect for both bacterial inoculants tested on IAA biosynthesis in comparison to non-inoculated seeds. Inoculation of maize seeds with VB2 and VB1 bacteria gave a significantly higher level of IAA production in comparison to non-inoculated seeds. Current study outcomes show the beneficial aspects of plant growth regulators produced by free-living bacteria which could play a significant role in plant growth promotion.https://www.termedia.pl/Production-of-indole-acetic-acid-by-Kocuria-rosea-VB1-and-Arthrobacter-luteolus-VB2-under-the-influence-of-L-tryptophan-and-maize-root-exudates,85,35923,1,1.html16s rdna sequencing phytohormones plant growth promotion rhizosphere waste water |
| spellingShingle | Arun Karnwal Production of indole acetic acid by Kocuria rosea VB1 and Arthrobacter luteolus VB2 under the influence of L-tryptophan and maize root exudates BioTechnologia 16s rdna sequencing phytohormones plant growth promotion rhizosphere waste water |
| title | Production of indole acetic acid by Kocuria rosea VB1 and Arthrobacter luteolus VB2 under the influence of L-tryptophan and maize root exudates |
| title_full | Production of indole acetic acid by Kocuria rosea VB1 and Arthrobacter luteolus VB2 under the influence of L-tryptophan and maize root exudates |
| title_fullStr | Production of indole acetic acid by Kocuria rosea VB1 and Arthrobacter luteolus VB2 under the influence of L-tryptophan and maize root exudates |
| title_full_unstemmed | Production of indole acetic acid by Kocuria rosea VB1 and Arthrobacter luteolus VB2 under the influence of L-tryptophan and maize root exudates |
| title_short | Production of indole acetic acid by Kocuria rosea VB1 and Arthrobacter luteolus VB2 under the influence of L-tryptophan and maize root exudates |
| title_sort | production of indole acetic acid by kocuria rosea vb1 and arthrobacter luteolus vb2 under the influence of l tryptophan and maize root exudates |
| topic | 16s rdna sequencing phytohormones plant growth promotion rhizosphere waste water |
| url | https://www.termedia.pl/Production-of-indole-acetic-acid-by-Kocuria-rosea-VB1-and-Arthrobacter-luteolus-VB2-under-the-influence-of-L-tryptophan-and-maize-root-exudates,85,35923,1,1.html |
| work_keys_str_mv | AT arunkarnwal productionofindoleaceticacidbykocuriaroseavb1andarthrobacterluteolusvb2undertheinfluenceofltryptophanandmaizerootexudates |