Protective Effect of Ginsenoside Rb1 Against Lung Injury Induced by Intestinal Ischemia-Reperfusion in Rats
Intestinal ischemia-reperfusion (I/R) is a critical event in the pathogenesis of multiple organ dysfunction syndromes (MODS). The lungs are some of the most vulnerable organs that are impacted by intestinal I/R. The aim of this study is to investigate whether ginsenoside Rb1 can ameliorate remote lu...
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MDPI AG
2013-01-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/18/1/1214 |
| _version_ | 1798046346975903744 |
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| author | Guangtian Yang Shusheng Li Lifen Qiao Jin Wang |
| author_facet | Guangtian Yang Shusheng Li Lifen Qiao Jin Wang |
| author_sort | Guangtian Yang |
| collection | DOAJ |
| description | Intestinal ischemia-reperfusion (I/R) is a critical event in the pathogenesis of multiple organ dysfunction syndromes (MODS). The lungs are some of the most vulnerable organs that are impacted by intestinal I/R. The aim of this study is to investigate whether ginsenoside Rb1 can ameliorate remote lung injury induced by intestinal I/R. Adult male Wistar rats were randomly divided into four groups: (1) a control, sham-operated group (sham group); (2) an intestinal I/R group subjected to 1 h intestinal ischemia and 2 h reperfusion (I/R group); (3) a group treated with 20 mg/kg ginsenoside Rb1 before reperfusion (Rb1-20 group); and (4) a group treated with 40 mg/kg ginsenoside Rb1 before reperfusion (Rb1-40 group). Intestinal and lung histology was observed. The malondialdehyde (MDA) levels in intestinal tissues were measured. Myeloperoxidase (MPO), TNF-α, MDA levels, wet/dry weight ratio and immunohistochemical expression of intracellular adhesion molecule-1 (ICAM-1) in lung tissues were assayed. In addition, a western blot of lung NF-kB was performed. Results indicated that intestinal I/R induced intestinal and lung injury, which was characterized by increase of MDA levels and pathological scores in intestinal tissues and MPO, TNF-α , MDA levels, wet/dry weight ratio and ICAM-1, NF-kB expression in the lung tissues. Ginsenoside Rb1 (20, 40 mg/kg) ameliorated intestinal and lung injury, decreased MPO, TNF-α, MDA levels, wet/dry weight ratio, ICAM-1 and NF-kB expression in lung tissues. In conclusion, ginsenoside Rb1 ameliorated the lung injuries by decreasing the NF-kB activation-induced inflammatory response. |
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| language | English |
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| spelling | doaj.art-d1edc84cb5d14e4ea0f06c3bf3a9d3792022-12-22T03:56:56ZengMDPI AGMolecules1420-30492013-01-011811214122610.3390/molecules18011214Protective Effect of Ginsenoside Rb1 Against Lung Injury Induced by Intestinal Ischemia-Reperfusion in RatsGuangtian YangShusheng LiLifen QiaoJin WangIntestinal ischemia-reperfusion (I/R) is a critical event in the pathogenesis of multiple organ dysfunction syndromes (MODS). The lungs are some of the most vulnerable organs that are impacted by intestinal I/R. The aim of this study is to investigate whether ginsenoside Rb1 can ameliorate remote lung injury induced by intestinal I/R. Adult male Wistar rats were randomly divided into four groups: (1) a control, sham-operated group (sham group); (2) an intestinal I/R group subjected to 1 h intestinal ischemia and 2 h reperfusion (I/R group); (3) a group treated with 20 mg/kg ginsenoside Rb1 before reperfusion (Rb1-20 group); and (4) a group treated with 40 mg/kg ginsenoside Rb1 before reperfusion (Rb1-40 group). Intestinal and lung histology was observed. The malondialdehyde (MDA) levels in intestinal tissues were measured. Myeloperoxidase (MPO), TNF-α, MDA levels, wet/dry weight ratio and immunohistochemical expression of intracellular adhesion molecule-1 (ICAM-1) in lung tissues were assayed. In addition, a western blot of lung NF-kB was performed. Results indicated that intestinal I/R induced intestinal and lung injury, which was characterized by increase of MDA levels and pathological scores in intestinal tissues and MPO, TNF-α , MDA levels, wet/dry weight ratio and ICAM-1, NF-kB expression in the lung tissues. Ginsenoside Rb1 (20, 40 mg/kg) ameliorated intestinal and lung injury, decreased MPO, TNF-α, MDA levels, wet/dry weight ratio, ICAM-1 and NF-kB expression in lung tissues. In conclusion, ginsenoside Rb1 ameliorated the lung injuries by decreasing the NF-kB activation-induced inflammatory response.http://www.mdpi.com/1420-3049/18/1/1214ischemia reperfusiontumor necrosis factor alphaintracellular adhesion molecule-1nuclear factor kappa Bginsenoside Rb1 |
| spellingShingle | Guangtian Yang Shusheng Li Lifen Qiao Jin Wang Protective Effect of Ginsenoside Rb1 Against Lung Injury Induced by Intestinal Ischemia-Reperfusion in Rats Molecules ischemia reperfusion tumor necrosis factor alpha intracellular adhesion molecule-1 nuclear factor kappa B ginsenoside Rb1 |
| title | Protective Effect of Ginsenoside Rb1 Against Lung Injury Induced by Intestinal Ischemia-Reperfusion in Rats |
| title_full | Protective Effect of Ginsenoside Rb1 Against Lung Injury Induced by Intestinal Ischemia-Reperfusion in Rats |
| title_fullStr | Protective Effect of Ginsenoside Rb1 Against Lung Injury Induced by Intestinal Ischemia-Reperfusion in Rats |
| title_full_unstemmed | Protective Effect of Ginsenoside Rb1 Against Lung Injury Induced by Intestinal Ischemia-Reperfusion in Rats |
| title_short | Protective Effect of Ginsenoside Rb1 Against Lung Injury Induced by Intestinal Ischemia-Reperfusion in Rats |
| title_sort | protective effect of ginsenoside rb1 against lung injury induced by intestinal ischemia reperfusion in rats |
| topic | ischemia reperfusion tumor necrosis factor alpha intracellular adhesion molecule-1 nuclear factor kappa B ginsenoside Rb1 |
| url | http://www.mdpi.com/1420-3049/18/1/1214 |
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