Helping proteins come in from the cold: 5 burning questions about cold-active enzymes
Enzymes from psychrophilic (cold-loving) organisms have attracted considerable interest over the past decades for their potential in various low-temperature industrial processes. However, we still lack large-scale commercialization of their activities. Here, we review their properties, limitations a...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | BBA Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667160323000339 |
| _version_ | 1827223324119269376 |
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| author | Jan Stanislaw Nowak Daniel E. Otzen |
| author_facet | Jan Stanislaw Nowak Daniel E. Otzen |
| author_sort | Jan Stanislaw Nowak |
| collection | DOAJ |
| description | Enzymes from psychrophilic (cold-loving) organisms have attracted considerable interest over the past decades for their potential in various low-temperature industrial processes. However, we still lack large-scale commercialization of their activities. Here, we review their properties, limitations and potential. Our review is structured around answers to 5 central questions: 1. How do cold-active enzymes achieve high catalytic rates at low temperatures? 2. How is protein flexibility connected to cold-activity? 3. What are the sequence-based and structural determinants for cold-activity? 4. How does the thermodynamic stability of psychrophilic enzymes reflect their cold-active capabilities? 5. How do we effectively identify novel cold-active enzymes, and can we apply them in an industrial context? We conclude that emerging screening technologies combined with big-data handling and analysis make it reasonable to expect a bright future for our understanding and exploitation of cold-active enzymes. |
| first_indexed | 2024-03-09T01:25:46Z |
| format | Article |
| id | doaj.art-d1f149f9fd174f839d44a5217386627a |
| institution | Directory Open Access Journal |
| issn | 2667-1603 |
| language | English |
| last_indexed | 2025-03-21T16:52:34Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | BBA Advances |
| spelling | doaj.art-d1f149f9fd174f839d44a5217386627a2024-06-15T06:14:26ZengElsevierBBA Advances2667-16032024-01-015100104Helping proteins come in from the cold: 5 burning questions about cold-active enzymesJan Stanislaw Nowak0Daniel E. Otzen1Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, DK – 8000 Aarhus C, DenmarkCorresponding author.; Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, DK – 8000 Aarhus C, DenmarkEnzymes from psychrophilic (cold-loving) organisms have attracted considerable interest over the past decades for their potential in various low-temperature industrial processes. However, we still lack large-scale commercialization of their activities. Here, we review their properties, limitations and potential. Our review is structured around answers to 5 central questions: 1. How do cold-active enzymes achieve high catalytic rates at low temperatures? 2. How is protein flexibility connected to cold-activity? 3. What are the sequence-based and structural determinants for cold-activity? 4. How does the thermodynamic stability of psychrophilic enzymes reflect their cold-active capabilities? 5. How do we effectively identify novel cold-active enzymes, and can we apply them in an industrial context? We conclude that emerging screening technologies combined with big-data handling and analysis make it reasonable to expect a bright future for our understanding and exploitation of cold-active enzymes.http://www.sciencedirect.com/science/article/pii/S2667160323000339PsychrophilicityEnzyme catalysisThermodynamic stabilityProtein dynamicsBioprospecting |
| spellingShingle | Jan Stanislaw Nowak Daniel E. Otzen Helping proteins come in from the cold: 5 burning questions about cold-active enzymes BBA Advances Psychrophilicity Enzyme catalysis Thermodynamic stability Protein dynamics Bioprospecting |
| title | Helping proteins come in from the cold: 5 burning questions about cold-active enzymes |
| title_full | Helping proteins come in from the cold: 5 burning questions about cold-active enzymes |
| title_fullStr | Helping proteins come in from the cold: 5 burning questions about cold-active enzymes |
| title_full_unstemmed | Helping proteins come in from the cold: 5 burning questions about cold-active enzymes |
| title_short | Helping proteins come in from the cold: 5 burning questions about cold-active enzymes |
| title_sort | helping proteins come in from the cold 5 burning questions about cold active enzymes |
| topic | Psychrophilicity Enzyme catalysis Thermodynamic stability Protein dynamics Bioprospecting |
| url | http://www.sciencedirect.com/science/article/pii/S2667160323000339 |
| work_keys_str_mv | AT janstanislawnowak helpingproteinscomeinfromthecold5burningquestionsaboutcoldactiveenzymes AT danieleotzen helpingproteinscomeinfromthecold5burningquestionsaboutcoldactiveenzymes |