Electrical Imaging of Light-Induced Signals Across and Within Retinal Layers
The mammalian retina processes sensory signals through two major pathways: a vertical excitatory pathway, which involves photoreceptors, bipolar cells, and ganglion cells, and a horizontal inhibitory pathway, which involves horizontal cells, and amacrine cells. This concept explains the generation o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-11-01
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| Series: | Frontiers in Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2020.563964/full |
| _version_ | 1818398718068523008 |
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| author | Meng-Jung Lee Meng-Jung Lee Günther Zeck |
| author_facet | Meng-Jung Lee Meng-Jung Lee Günther Zeck |
| author_sort | Meng-Jung Lee |
| collection | DOAJ |
| description | The mammalian retina processes sensory signals through two major pathways: a vertical excitatory pathway, which involves photoreceptors, bipolar cells, and ganglion cells, and a horizontal inhibitory pathway, which involves horizontal cells, and amacrine cells. This concept explains the generation of an excitatory center—inhibitory surround sensory receptive fields—but fails to explain the modulation of the retinal output by stimuli outside the receptive field. Electrical imaging of light-induced signal propagation at high spatial and temporal resolution across and within different retinal layers might reveal mechanisms and circuits involved in the remote modulation of the retinal output. Here we took advantage of a high-density complementary metal oxide semiconductor-based microelectrode array and investigated the light-induced propagation of local field potentials (LFPs) in vertical mouse retina slices. Surprisingly, the LFP propagation within the different retinal layers depends on stimulus duration and stimulus background. Application of the same spatially restricted light stimuli to flat-mounted retina induced ganglion cell activity at remote distances from the stimulus center. This effect disappeared if a global background was provided or if gap junctions were blocked. We hereby present a neurotechnological approach and demonstrated its application, in which electrical imaging evaluates stimulus-dependent signal processing across different neural layers. |
| first_indexed | 2024-12-14T07:09:14Z |
| format | Article |
| id | doaj.art-d1f386a5010344adadff75bbb04bb2fc |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-12-14T07:09:14Z |
| publishDate | 2020-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-d1f386a5010344adadff75bbb04bb2fc2022-12-21T23:12:01ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2020-11-011410.3389/fnins.2020.563964563964Electrical Imaging of Light-Induced Signals Across and Within Retinal LayersMeng-Jung Lee0Meng-Jung Lee1Günther Zeck2Neurophysics, NMI Natural and Medical Sciences Institute at the University Tübingen, Reutlingen, GermanyGraduate School of Neural Information Processing, International Max Planck Research School, Tübingen, GermanyNeurophysics, NMI Natural and Medical Sciences Institute at the University Tübingen, Reutlingen, GermanyThe mammalian retina processes sensory signals through two major pathways: a vertical excitatory pathway, which involves photoreceptors, bipolar cells, and ganglion cells, and a horizontal inhibitory pathway, which involves horizontal cells, and amacrine cells. This concept explains the generation of an excitatory center—inhibitory surround sensory receptive fields—but fails to explain the modulation of the retinal output by stimuli outside the receptive field. Electrical imaging of light-induced signal propagation at high spatial and temporal resolution across and within different retinal layers might reveal mechanisms and circuits involved in the remote modulation of the retinal output. Here we took advantage of a high-density complementary metal oxide semiconductor-based microelectrode array and investigated the light-induced propagation of local field potentials (LFPs) in vertical mouse retina slices. Surprisingly, the LFP propagation within the different retinal layers depends on stimulus duration and stimulus background. Application of the same spatially restricted light stimuli to flat-mounted retina induced ganglion cell activity at remote distances from the stimulus center. This effect disappeared if a global background was provided or if gap junctions were blocked. We hereby present a neurotechnological approach and demonstrated its application, in which electrical imaging evaluates stimulus-dependent signal processing across different neural layers.https://www.frontiersin.org/articles/10.3389/fnins.2020.563964/fullretinaretinal sliceCMOS-MEAmicroelectrode arrayelectrical imaging |
| spellingShingle | Meng-Jung Lee Meng-Jung Lee Günther Zeck Electrical Imaging of Light-Induced Signals Across and Within Retinal Layers Frontiers in Neuroscience retina retinal slice CMOS-MEA microelectrode array electrical imaging |
| title | Electrical Imaging of Light-Induced Signals Across and Within Retinal Layers |
| title_full | Electrical Imaging of Light-Induced Signals Across and Within Retinal Layers |
| title_fullStr | Electrical Imaging of Light-Induced Signals Across and Within Retinal Layers |
| title_full_unstemmed | Electrical Imaging of Light-Induced Signals Across and Within Retinal Layers |
| title_short | Electrical Imaging of Light-Induced Signals Across and Within Retinal Layers |
| title_sort | electrical imaging of light induced signals across and within retinal layers |
| topic | retina retinal slice CMOS-MEA microelectrode array electrical imaging |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2020.563964/full |
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