Above the Invasive and Ornamental Attributes of the Traveler’s Palm: An In Vitro and In Silico Insight into the Anti-Oxidant, Anti-Enzymatic, Cytotoxic and Phytochemical Characterization of <i>Ravenala madagascariensis</i>
<i>Ravenala madagascariensis</i> is a widely known ornamental and medicinal plant, but with a dearth of scientific investigations regarding its phytochemical and pharmacological properties. Hence, these properties were appraised in this study. The DPPH (154.08 ± 2.43 mgTE/g), FRAP (249.4...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/1/184 |
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| author | Shanoo Suroowan Eulogio Jose Llorent-Martínez Gokhan Zengin Stefano Dall’Acqua Stefania Sut Kalaivani Buskaran Sharida Fakurazi Bao Le Van Mohnad Abdalla Ashraf N. Abdalla Asaad Khalid Mohamad Fawzi Mahomoodally |
| author_facet | Shanoo Suroowan Eulogio Jose Llorent-Martínez Gokhan Zengin Stefano Dall’Acqua Stefania Sut Kalaivani Buskaran Sharida Fakurazi Bao Le Van Mohnad Abdalla Ashraf N. Abdalla Asaad Khalid Mohamad Fawzi Mahomoodally |
| author_sort | Shanoo Suroowan |
| collection | DOAJ |
| description | <i>Ravenala madagascariensis</i> is a widely known ornamental and medicinal plant, but with a dearth of scientific investigations regarding its phytochemical and pharmacological properties. Hence, these properties were appraised in this study. The DPPH (154.08 ± 2.43 mgTE/g), FRAP (249.40 ± 3.01 mgTE/g), CUPRAC (384.57 ± 1.99 mgTE/g), metal chelating (29.68 ± 0.74 mgEDTAE/g) and phosphomolybdenum assay (2.38 ± 0.07 mmolTE/g) results demonstrated that the aqueous extract had the most prominent antioxidant activity, while the methanolic extract displayed the best antioxidant potential in the ABTS assay (438.46 ± 1.69 mgTE/g). The HPLC-ESI-Q-TOF-MS-MS analysis allowed the characterization of 41 metabolites. The methanolic extract was the most active against acetylcholinesterase. All extracts were active against the alpha-amylase and alpha-glucosidase enzymes, with the ethyl acetate extract being the most active against the alpha-amylase enzyme, while the methanolic extract showed the best alpha-glucosidase inhibition. A plethora of metabolites bonded more energetically with the assayed enzymes active sites based on the results of the in silico studies. <i>R. madagascariensis</i> extracts used in this study exhibited cytotoxicity against HT29 cells. The IC<sub>50</sub> of the methanolic extract was lower (506.99 ug/mL). Based on the heat map, whereby flavonoids were found to be in greater proportion in the extracts, it can be concluded that the flavonoid portion of the extracts contributed to the most activity. |
| first_indexed | 2024-03-09T13:46:36Z |
| format | Article |
| id | doaj.art-d1f3f989db2943b6b071789819f0d3ef |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T13:46:36Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-d1f3f989db2943b6b071789819f0d3ef2023-11-30T20:59:32ZengMDPI AGAntioxidants2076-39212023-01-0112118410.3390/antiox12010184Above the Invasive and Ornamental Attributes of the Traveler’s Palm: An In Vitro and In Silico Insight into the Anti-Oxidant, Anti-Enzymatic, Cytotoxic and Phytochemical Characterization of <i>Ravenala madagascariensis</i>Shanoo Suroowan0Eulogio Jose Llorent-Martínez1Gokhan Zengin2Stefano Dall’Acqua3Stefania Sut4Kalaivani Buskaran5Sharida Fakurazi6Bao Le Van7Mohnad Abdalla8Ashraf N. Abdalla9Asaad Khalid10Mohamad Fawzi Mahomoodally11Department of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Réduit 80837, MauritiusDepartment of Physical and Analytical Chemistry, University of Jaén, Campus Las Lagunillas S/N, E-23071 Jaén, SpainDepartment of Biology, Science Faculty, Selcuk University, Konya 42130, TurkeyDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, ItalyDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, ItalyLaboratory of Natural Medicine and Product Research, Institute of Bioscience, Universiti Putra Malaysia, Serdang 43400, Selangor, MalaysiaLaboratory of Natural Medicine and Product Research, Institute of Bioscience, Universiti Putra Malaysia, Serdang 43400, Selangor, MalaysiaInstitute of Research and Development, Duy Tan University, Da Nang 550000, VietnamPediatric Research Institute, Children’s Hospital Affiliated to Shandong University, Jinan 250022, ChinaDepartment of Pharmacology and Toxicology, College of Pharmacy, Umm Al-Qura University, Makkah 21955, Saudi ArabiaSubstance Abuse and Toxicology Research Center, Jazan University, P.O. Box 114, Jazan 45142, Saudi ArabiaDepartment of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Réduit 80837, Mauritius<i>Ravenala madagascariensis</i> is a widely known ornamental and medicinal plant, but with a dearth of scientific investigations regarding its phytochemical and pharmacological properties. Hence, these properties were appraised in this study. The DPPH (154.08 ± 2.43 mgTE/g), FRAP (249.40 ± 3.01 mgTE/g), CUPRAC (384.57 ± 1.99 mgTE/g), metal chelating (29.68 ± 0.74 mgEDTAE/g) and phosphomolybdenum assay (2.38 ± 0.07 mmolTE/g) results demonstrated that the aqueous extract had the most prominent antioxidant activity, while the methanolic extract displayed the best antioxidant potential in the ABTS assay (438.46 ± 1.69 mgTE/g). The HPLC-ESI-Q-TOF-MS-MS analysis allowed the characterization of 41 metabolites. The methanolic extract was the most active against acetylcholinesterase. All extracts were active against the alpha-amylase and alpha-glucosidase enzymes, with the ethyl acetate extract being the most active against the alpha-amylase enzyme, while the methanolic extract showed the best alpha-glucosidase inhibition. A plethora of metabolites bonded more energetically with the assayed enzymes active sites based on the results of the in silico studies. <i>R. madagascariensis</i> extracts used in this study exhibited cytotoxicity against HT29 cells. The IC<sub>50</sub> of the methanolic extract was lower (506.99 ug/mL). Based on the heat map, whereby flavonoids were found to be in greater proportion in the extracts, it can be concluded that the flavonoid portion of the extracts contributed to the most activity.https://www.mdpi.com/2076-3921/12/1/184<i>Ravenala madagascariensis</i>antioxidantsenzyme inhibitionnatural agents |
| spellingShingle | Shanoo Suroowan Eulogio Jose Llorent-Martínez Gokhan Zengin Stefano Dall’Acqua Stefania Sut Kalaivani Buskaran Sharida Fakurazi Bao Le Van Mohnad Abdalla Ashraf N. Abdalla Asaad Khalid Mohamad Fawzi Mahomoodally Above the Invasive and Ornamental Attributes of the Traveler’s Palm: An In Vitro and In Silico Insight into the Anti-Oxidant, Anti-Enzymatic, Cytotoxic and Phytochemical Characterization of <i>Ravenala madagascariensis</i> Antioxidants <i>Ravenala madagascariensis</i> antioxidants enzyme inhibition natural agents |
| title | Above the Invasive and Ornamental Attributes of the Traveler’s Palm: An In Vitro and In Silico Insight into the Anti-Oxidant, Anti-Enzymatic, Cytotoxic and Phytochemical Characterization of <i>Ravenala madagascariensis</i> |
| title_full | Above the Invasive and Ornamental Attributes of the Traveler’s Palm: An In Vitro and In Silico Insight into the Anti-Oxidant, Anti-Enzymatic, Cytotoxic and Phytochemical Characterization of <i>Ravenala madagascariensis</i> |
| title_fullStr | Above the Invasive and Ornamental Attributes of the Traveler’s Palm: An In Vitro and In Silico Insight into the Anti-Oxidant, Anti-Enzymatic, Cytotoxic and Phytochemical Characterization of <i>Ravenala madagascariensis</i> |
| title_full_unstemmed | Above the Invasive and Ornamental Attributes of the Traveler’s Palm: An In Vitro and In Silico Insight into the Anti-Oxidant, Anti-Enzymatic, Cytotoxic and Phytochemical Characterization of <i>Ravenala madagascariensis</i> |
| title_short | Above the Invasive and Ornamental Attributes of the Traveler’s Palm: An In Vitro and In Silico Insight into the Anti-Oxidant, Anti-Enzymatic, Cytotoxic and Phytochemical Characterization of <i>Ravenala madagascariensis</i> |
| title_sort | above the invasive and ornamental attributes of the traveler s palm an in vitro and in silico insight into the anti oxidant anti enzymatic cytotoxic and phytochemical characterization of i ravenala madagascariensis i |
| topic | <i>Ravenala madagascariensis</i> antioxidants enzyme inhibition natural agents |
| url | https://www.mdpi.com/2076-3921/12/1/184 |
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