Porphyromonas gingivalis W83 traffics via ICAM1 in microvascular endothelial cells and alters capillary organization in vivo
Objective: Microvascular dysfunction is a feature of periodontal disease. P. gingivalis, one of the most common oral bacteria present in gingival tissue biofilms, has also been identified in the gingival capillaries of patients with chronic periodontitis. We sought to determine the effect of P. ging...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Journal of Oral Microbiology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/20002297.2020.1742528 |
| _version_ | 1818827265885077504 |
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| author | L. Reyes H. Getachew W.A. Dunn A. Progulske-Fox |
| author_facet | L. Reyes H. Getachew W.A. Dunn A. Progulske-Fox |
| author_sort | L. Reyes |
| collection | DOAJ |
| description | Objective: Microvascular dysfunction is a feature of periodontal disease. P. gingivalis, one of the most common oral bacteria present in gingival tissue biofilms, has also been identified in the gingival capillaries of patients with chronic periodontitis. We sought to determine the effect of P. gingivalis W83 infection on microvascular endothelium in vivo and in vitro. Methods and Results: Interdental papillae of rats with P. gingivalis-induced alveolar bone loss had a more dilated and denser subepithelial capillary network than uninfected controls. P. gingivalis W83 was detected in the epithelial layers, the subepithelial connective tissue matrix, and subgingival capillaries. P. gingivalis invaded human dermal microvascular endothelial cells (HD-MVECS) and persisted up termination (24 h). Colocalization analysis at 2.5, 6, and 24 h post-inoculation showed that 79–88% of internalized bacteria were in ICAM-1 positive endosomes, and 10–39% were in Rab5, Rab7, or LAMP1 positive compartments, but never in autophagosomes. Antibody-based blockade of ICAM-1 significantly reduced W83 invasion in HD-MVECS. P. gingivalis infected HD-MVECS were unable to form vascular networks in Matrigel. Conclusions: P. gingivalis perturbs microvascular endothelial function and invasion of these cells via ICAM-1 may be important for microbial persistence within tissues. |
| first_indexed | 2024-12-19T00:40:49Z |
| format | Article |
| id | doaj.art-d1f8f87d5e534f2a9fd1e8f9cf737a5c |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-19T00:40:49Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-d1f8f87d5e534f2a9fd1e8f9cf737a5c2022-12-21T20:44:35ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972020-01-0112110.1080/20002297.2020.17425281742528Porphyromonas gingivalis W83 traffics via ICAM1 in microvascular endothelial cells and alters capillary organization in vivoL. Reyes0H. Getachew1W.A. Dunn2A. Progulske-Fox3University of Wisconsin - Madison, School of Veterinary MedicineCenter for Molecular Microbiology University of FloridaCenter for Molecular Microbiology University of FloridaCenter for Molecular Microbiology University of FloridaObjective: Microvascular dysfunction is a feature of periodontal disease. P. gingivalis, one of the most common oral bacteria present in gingival tissue biofilms, has also been identified in the gingival capillaries of patients with chronic periodontitis. We sought to determine the effect of P. gingivalis W83 infection on microvascular endothelium in vivo and in vitro. Methods and Results: Interdental papillae of rats with P. gingivalis-induced alveolar bone loss had a more dilated and denser subepithelial capillary network than uninfected controls. P. gingivalis W83 was detected in the epithelial layers, the subepithelial connective tissue matrix, and subgingival capillaries. P. gingivalis invaded human dermal microvascular endothelial cells (HD-MVECS) and persisted up termination (24 h). Colocalization analysis at 2.5, 6, and 24 h post-inoculation showed that 79–88% of internalized bacteria were in ICAM-1 positive endosomes, and 10–39% were in Rab5, Rab7, or LAMP1 positive compartments, but never in autophagosomes. Antibody-based blockade of ICAM-1 significantly reduced W83 invasion in HD-MVECS. P. gingivalis infected HD-MVECS were unable to form vascular networks in Matrigel. Conclusions: P. gingivalis perturbs microvascular endothelial function and invasion of these cells via ICAM-1 may be important for microbial persistence within tissues.http://dx.doi.org/10.1080/20002297.2020.1742528periodontitismicrovascular endotheliumporphyromonas gingivalisicam-1 |
| spellingShingle | L. Reyes H. Getachew W.A. Dunn A. Progulske-Fox Porphyromonas gingivalis W83 traffics via ICAM1 in microvascular endothelial cells and alters capillary organization in vivo Journal of Oral Microbiology periodontitis microvascular endothelium porphyromonas gingivalis icam-1 |
| title | Porphyromonas gingivalis W83 traffics via ICAM1 in microvascular endothelial cells and alters capillary organization in vivo |
| title_full | Porphyromonas gingivalis W83 traffics via ICAM1 in microvascular endothelial cells and alters capillary organization in vivo |
| title_fullStr | Porphyromonas gingivalis W83 traffics via ICAM1 in microvascular endothelial cells and alters capillary organization in vivo |
| title_full_unstemmed | Porphyromonas gingivalis W83 traffics via ICAM1 in microvascular endothelial cells and alters capillary organization in vivo |
| title_short | Porphyromonas gingivalis W83 traffics via ICAM1 in microvascular endothelial cells and alters capillary organization in vivo |
| title_sort | porphyromonas gingivalis w83 traffics via icam1 in microvascular endothelial cells and alters capillary organization in vivo |
| topic | periodontitis microvascular endothelium porphyromonas gingivalis icam-1 |
| url | http://dx.doi.org/10.1080/20002297.2020.1742528 |
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