<i>RTG</i> Signaling Sustains Mitochondrial Respiratory Capacity in <i>HOG1</i>-Dependent Osmoadaptation
Mitochondrial <i>RTG</i>-dependent retrograde signaling, whose regulators have been characterized in <i>Saccharomyces cerevisiae</i>, plays a recognized role under various environmental stresses. Of special significance, the activity of the transcriptional complex Rtg1/3 has...
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MDPI AG
2021-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/9/1894 |
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| author | Nicoletta Guaragnella Gennaro Agrimi Pasquale Scarcia Clelia Suriano Isabella Pisano Antonella Bobba Cristina Mazzoni Luigi Palmieri Sergio Giannattasio |
| author_facet | Nicoletta Guaragnella Gennaro Agrimi Pasquale Scarcia Clelia Suriano Isabella Pisano Antonella Bobba Cristina Mazzoni Luigi Palmieri Sergio Giannattasio |
| author_sort | Nicoletta Guaragnella |
| collection | DOAJ |
| description | Mitochondrial <i>RTG</i>-dependent retrograde signaling, whose regulators have been characterized in <i>Saccharomyces cerevisiae</i>, plays a recognized role under various environmental stresses. Of special significance, the activity of the transcriptional complex Rtg1/3 has been shown to be modulated by Hog1, the master regulator of the high osmolarity glycerol pathway, in response to osmotic stress. The present work focuses on the role of <i>RTG</i> signaling in salt-induced osmotic stress and its interaction with <i>HOG1</i>. Wild-type and mutant cells, lacking <i>HOG1</i> and/or <i>RTG</i> genes, are compared with respect to cell growth features, retrograde signaling activation and mitochondrial function in the presence and in the absence of high osmostress. We show that <i>RTG2,</i> the main upstream regulator of the <i>RTG</i> pathway, contributes to osmoadaptation in an <i>HOG1</i>-dependent manner and that, with <i>RTG3</i>, it is notably involved in a late phase of growth. Our data demonstrate that impairment of <i>RTG</i> signaling causes a decrease in mitochondrial respiratory capacity exclusively under osmostress. Overall, these results suggest that <i>HOG1</i> and the <i>RTG</i> pathway may interact sequentially in the stress signaling cascade and that the <i>RTG</i> pathway may play a role in inter-organellar metabolic communication for osmoadaptation. |
| first_indexed | 2024-03-10T07:25:30Z |
| format | Article |
| id | doaj.art-d1f9bf3a5a4d4038b25640de122e535a |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T07:25:30Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-d1f9bf3a5a4d4038b25640de122e535a2023-11-22T14:18:42ZengMDPI AGMicroorganisms2076-26072021-09-0199189410.3390/microorganisms9091894<i>RTG</i> Signaling Sustains Mitochondrial Respiratory Capacity in <i>HOG1</i>-Dependent OsmoadaptationNicoletta Guaragnella0Gennaro Agrimi1Pasquale Scarcia2Clelia Suriano3Isabella Pisano4Antonella Bobba5Cristina Mazzoni6Luigi Palmieri7Sergio Giannattasio8Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari “Aldo Moro”, 70125 Bari, ItalyDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari “Aldo Moro”, 70125 Bari, ItalyDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari “Aldo Moro”, 70125 Bari, ItalyDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari “Aldo Moro”, 70125 Bari, ItalyDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari “Aldo Moro”, 70125 Bari, ItalyInstitute of Biomembranes, Bioenergetics and Molecular Biotechnologies, National Research Council, 70125 Bari, ItalyDepartment of Biology and Biotechnology ‘Charles Darwin’, Pasteur Institute-Cenci Bolognetti Foundation, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari “Aldo Moro”, 70125 Bari, ItalyInstitute of Biomembranes, Bioenergetics and Molecular Biotechnologies, National Research Council, 70125 Bari, ItalyMitochondrial <i>RTG</i>-dependent retrograde signaling, whose regulators have been characterized in <i>Saccharomyces cerevisiae</i>, plays a recognized role under various environmental stresses. Of special significance, the activity of the transcriptional complex Rtg1/3 has been shown to be modulated by Hog1, the master regulator of the high osmolarity glycerol pathway, in response to osmotic stress. The present work focuses on the role of <i>RTG</i> signaling in salt-induced osmotic stress and its interaction with <i>HOG1</i>. Wild-type and mutant cells, lacking <i>HOG1</i> and/or <i>RTG</i> genes, are compared with respect to cell growth features, retrograde signaling activation and mitochondrial function in the presence and in the absence of high osmostress. We show that <i>RTG2,</i> the main upstream regulator of the <i>RTG</i> pathway, contributes to osmoadaptation in an <i>HOG1</i>-dependent manner and that, with <i>RTG3</i>, it is notably involved in a late phase of growth. Our data demonstrate that impairment of <i>RTG</i> signaling causes a decrease in mitochondrial respiratory capacity exclusively under osmostress. Overall, these results suggest that <i>HOG1</i> and the <i>RTG</i> pathway may interact sequentially in the stress signaling cascade and that the <i>RTG</i> pathway may play a role in inter-organellar metabolic communication for osmoadaptation.https://www.mdpi.com/2076-2607/9/9/1894<i>RTG</i> signaling<i>HOG1</i>osmoadaptationmitochondriarespiratory capacitystress response |
| spellingShingle | Nicoletta Guaragnella Gennaro Agrimi Pasquale Scarcia Clelia Suriano Isabella Pisano Antonella Bobba Cristina Mazzoni Luigi Palmieri Sergio Giannattasio <i>RTG</i> Signaling Sustains Mitochondrial Respiratory Capacity in <i>HOG1</i>-Dependent Osmoadaptation Microorganisms <i>RTG</i> signaling <i>HOG1</i> osmoadaptation mitochondria respiratory capacity stress response |
| title | <i>RTG</i> Signaling Sustains Mitochondrial Respiratory Capacity in <i>HOG1</i>-Dependent Osmoadaptation |
| title_full | <i>RTG</i> Signaling Sustains Mitochondrial Respiratory Capacity in <i>HOG1</i>-Dependent Osmoadaptation |
| title_fullStr | <i>RTG</i> Signaling Sustains Mitochondrial Respiratory Capacity in <i>HOG1</i>-Dependent Osmoadaptation |
| title_full_unstemmed | <i>RTG</i> Signaling Sustains Mitochondrial Respiratory Capacity in <i>HOG1</i>-Dependent Osmoadaptation |
| title_short | <i>RTG</i> Signaling Sustains Mitochondrial Respiratory Capacity in <i>HOG1</i>-Dependent Osmoadaptation |
| title_sort | i rtg i signaling sustains mitochondrial respiratory capacity in i hog1 i dependent osmoadaptation |
| topic | <i>RTG</i> signaling <i>HOG1</i> osmoadaptation mitochondria respiratory capacity stress response |
| url | https://www.mdpi.com/2076-2607/9/9/1894 |
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