Sub-Lethal Effects of <i>Lecanicillium lecanii</i> (Zimmermann)-Derived Partially Purified Protein and Its Potential Implication in Cotton (<i>Gossypium hirsutum</i> L.) Defense against <i>Bemisia tabaci</i> Gennadius (Aleyrodidae: Hemiptera)
Whiteflies, <i>Bemisia tabaci</i> Gennadius (Aleyrodidae: Hemiptera), are a polyphagous economically destructive pest of several solanaceous crops around the world. Many secondary metabolites are synthesized by different biotrophic and necrotrophic fungi which are capable of inducing sys...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/11/8/778 |
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| author | Yusuf Ali Abdulle Talha Nazir Samy Sayed Samy F. Mahmoud Muhammad Zeeshan Majeed Hafiz Muhammad Usman Aslam Zubair Iqbal Muhammad Shahid Nisar Azhar Uddin Keerio Habib Ali Dewen Qiu |
| author_facet | Yusuf Ali Abdulle Talha Nazir Samy Sayed Samy F. Mahmoud Muhammad Zeeshan Majeed Hafiz Muhammad Usman Aslam Zubair Iqbal Muhammad Shahid Nisar Azhar Uddin Keerio Habib Ali Dewen Qiu |
| author_sort | Yusuf Ali Abdulle |
| collection | DOAJ |
| description | Whiteflies, <i>Bemisia tabaci</i> Gennadius (Aleyrodidae: Hemiptera), are a polyphagous economically destructive pest of several solanaceous crops around the world. Many secondary metabolites are synthesized by different biotrophic and necrotrophic fungi which are capable of inducing systemic resistance in plants against various phytophagous pests. The present laboratory work demonstrated the anti-insect impact of a protein extracted and purified partially from an entomopathogenic fungus (EPF) <i>Lecanicillium lecanii</i> (Zimmermann) against <i>B. tabaci</i>. Three different concentrations (i.e., 7.43, 11.15, and 22.31 μg mL<sup>−1</sup>) of this protein were bioassayed to assess its effect on the fecundity rate of <i>B. tabaci</i> on cotton (<i>Gossypium hirsutum</i> L.) plants. Furthermore, the possible implication of this fungal protein in defense pathways of cotton plants was evaluated by determining the expression profiles of salicylic acid (SA) and jasmonic acid (JA) pathways related to major genes through reverse transcription qPCR (RT-qPCR). According to the results, all protein concentrations exerted a significant (F<sub>3, 252</sub> = 62.51; <i>p</i> ≤ 0.001) and negative impact on the fecundity rate of <i>B. tabaci</i> females. At the highest protein concentration (22.31 μg mL<sup>−1</sup>), the minimum rate of fecundity (i.e., 2.46 eggs female<sup>−1</sup>day<sup>−1</sup>) of <i>B. tabaci</i> was noted on the seventh day, whereas fecundity rates for the other two protein concentrations (i.e., 11.15 and 7.43 μg mL<sup>−1</sup>) were, respectively, 3.06 and 3.90 eggs day<sup>−1</sup> female<sup>−1</sup>. The maximum rate of fecundity (6.01 eggs female<sup>−1</sup>day<sup>−1</sup>) was recorded in untreated (control) treatments. In addition, the foliar application of <i>L. lecanii</i> derived protein significantly upregulated all SA linked genes (OPR3, PPO1 and COI1) and slightly triggered up the JA linked genes (LOX1, UBQ7 and AOS) in the cotton plants. These findings revealed that this <i>L. lecanii</i> extracted partially purified protein triggered systemic resistance against <i>B. tabaci</i> in the cotton plants, proposing its putative effectiveness as an innovative biological control technique against <i>B. tabaci</i> and other phloem-feeding hemipteran pests. Nevertheless, further investigations such as purification and molecular and functional characterization of this <i>L. lecanii</i>-derived partially purified protein are required. |
| first_indexed | 2024-03-10T09:06:39Z |
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| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-10T09:06:39Z |
| publishDate | 2021-08-01 |
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| series | Agriculture |
| spelling | doaj.art-d1fae25e3afc4e28a08e1e55db3fb9372023-11-22T06:23:33ZengMDPI AGAgriculture2077-04722021-08-0111877810.3390/agriculture11080778Sub-Lethal Effects of <i>Lecanicillium lecanii</i> (Zimmermann)-Derived Partially Purified Protein and Its Potential Implication in Cotton (<i>Gossypium hirsutum</i> L.) Defense against <i>Bemisia tabaci</i> Gennadius (Aleyrodidae: Hemiptera)Yusuf Ali Abdulle0Talha Nazir1Samy Sayed2Samy F. Mahmoud3Muhammad Zeeshan Majeed4Hafiz Muhammad Usman Aslam5Zubair Iqbal6Muhammad Shahid Nisar7Azhar Uddin Keerio8Habib Ali9Dewen Qiu10State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaDepartment of Science and Technology, University College-Ranyah, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Entomology, College of Agriculture, University of Sargodha, Sargodha 40100, PakistanDepartment of Plant Pathology, Institute of Plant Protection (IPP), MNS-University of Agriculture, Multan 60650, PakistanState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaDepartment of Plant Protection, Faculty of Agricultural Sciences, Ghazi University, Dera Ghazi Khan 32200, PakistanState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaDepartment of Agricultural Engineering, Khawaja Fareed University of Engineering and Information Technology, Rahim Yar Khan 64200, PakistanState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaWhiteflies, <i>Bemisia tabaci</i> Gennadius (Aleyrodidae: Hemiptera), are a polyphagous economically destructive pest of several solanaceous crops around the world. Many secondary metabolites are synthesized by different biotrophic and necrotrophic fungi which are capable of inducing systemic resistance in plants against various phytophagous pests. The present laboratory work demonstrated the anti-insect impact of a protein extracted and purified partially from an entomopathogenic fungus (EPF) <i>Lecanicillium lecanii</i> (Zimmermann) against <i>B. tabaci</i>. Three different concentrations (i.e., 7.43, 11.15, and 22.31 μg mL<sup>−1</sup>) of this protein were bioassayed to assess its effect on the fecundity rate of <i>B. tabaci</i> on cotton (<i>Gossypium hirsutum</i> L.) plants. Furthermore, the possible implication of this fungal protein in defense pathways of cotton plants was evaluated by determining the expression profiles of salicylic acid (SA) and jasmonic acid (JA) pathways related to major genes through reverse transcription qPCR (RT-qPCR). According to the results, all protein concentrations exerted a significant (F<sub>3, 252</sub> = 62.51; <i>p</i> ≤ 0.001) and negative impact on the fecundity rate of <i>B. tabaci</i> females. At the highest protein concentration (22.31 μg mL<sup>−1</sup>), the minimum rate of fecundity (i.e., 2.46 eggs female<sup>−1</sup>day<sup>−1</sup>) of <i>B. tabaci</i> was noted on the seventh day, whereas fecundity rates for the other two protein concentrations (i.e., 11.15 and 7.43 μg mL<sup>−1</sup>) were, respectively, 3.06 and 3.90 eggs day<sup>−1</sup> female<sup>−1</sup>. The maximum rate of fecundity (6.01 eggs female<sup>−1</sup>day<sup>−1</sup>) was recorded in untreated (control) treatments. In addition, the foliar application of <i>L. lecanii</i> derived protein significantly upregulated all SA linked genes (OPR3, PPO1 and COI1) and slightly triggered up the JA linked genes (LOX1, UBQ7 and AOS) in the cotton plants. These findings revealed that this <i>L. lecanii</i> extracted partially purified protein triggered systemic resistance against <i>B. tabaci</i> in the cotton plants, proposing its putative effectiveness as an innovative biological control technique against <i>B. tabaci</i> and other phloem-feeding hemipteran pests. Nevertheless, further investigations such as purification and molecular and functional characterization of this <i>L. lecanii</i>-derived partially purified protein are required.https://www.mdpi.com/2077-0472/11/8/778<i>Lecanicillium lecanii</i>induced resistance<i>Gossypium hirsutum</i><i>Bemisia tabaci</i>fecunditysalicylic acid pathway |
| spellingShingle | Yusuf Ali Abdulle Talha Nazir Samy Sayed Samy F. Mahmoud Muhammad Zeeshan Majeed Hafiz Muhammad Usman Aslam Zubair Iqbal Muhammad Shahid Nisar Azhar Uddin Keerio Habib Ali Dewen Qiu Sub-Lethal Effects of <i>Lecanicillium lecanii</i> (Zimmermann)-Derived Partially Purified Protein and Its Potential Implication in Cotton (<i>Gossypium hirsutum</i> L.) Defense against <i>Bemisia tabaci</i> Gennadius (Aleyrodidae: Hemiptera) Agriculture <i>Lecanicillium lecanii</i> induced resistance <i>Gossypium hirsutum</i> <i>Bemisia tabaci</i> fecundity salicylic acid pathway |
| title | Sub-Lethal Effects of <i>Lecanicillium lecanii</i> (Zimmermann)-Derived Partially Purified Protein and Its Potential Implication in Cotton (<i>Gossypium hirsutum</i> L.) Defense against <i>Bemisia tabaci</i> Gennadius (Aleyrodidae: Hemiptera) |
| title_full | Sub-Lethal Effects of <i>Lecanicillium lecanii</i> (Zimmermann)-Derived Partially Purified Protein and Its Potential Implication in Cotton (<i>Gossypium hirsutum</i> L.) Defense against <i>Bemisia tabaci</i> Gennadius (Aleyrodidae: Hemiptera) |
| title_fullStr | Sub-Lethal Effects of <i>Lecanicillium lecanii</i> (Zimmermann)-Derived Partially Purified Protein and Its Potential Implication in Cotton (<i>Gossypium hirsutum</i> L.) Defense against <i>Bemisia tabaci</i> Gennadius (Aleyrodidae: Hemiptera) |
| title_full_unstemmed | Sub-Lethal Effects of <i>Lecanicillium lecanii</i> (Zimmermann)-Derived Partially Purified Protein and Its Potential Implication in Cotton (<i>Gossypium hirsutum</i> L.) Defense against <i>Bemisia tabaci</i> Gennadius (Aleyrodidae: Hemiptera) |
| title_short | Sub-Lethal Effects of <i>Lecanicillium lecanii</i> (Zimmermann)-Derived Partially Purified Protein and Its Potential Implication in Cotton (<i>Gossypium hirsutum</i> L.) Defense against <i>Bemisia tabaci</i> Gennadius (Aleyrodidae: Hemiptera) |
| title_sort | sub lethal effects of i lecanicillium lecanii i zimmermann derived partially purified protein and its potential implication in cotton i gossypium hirsutum i l defense against i bemisia tabaci i gennadius aleyrodidae hemiptera |
| topic | <i>Lecanicillium lecanii</i> induced resistance <i>Gossypium hirsutum</i> <i>Bemisia tabaci</i> fecundity salicylic acid pathway |
| url | https://www.mdpi.com/2077-0472/11/8/778 |
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