Long noncoding RNA LIPH-4 promotes esophageal squamous cell carcinoma progression by regulating the miR-216b/IGF2BP2 axis
Abstract Introduction Esophageal squamous cell carcinoma (ESCC) represents a major malignancy with poor clinical outcomes. Long noncoding RNAs (lncRNAs) are known to regulate the development and progression of multiple cancers. However, how lncRNAs are involved in ESCC is currently undefined. Method...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-08-01
|
| Series: | Biomarker Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40364-022-00408-x |
| _version_ | 1798038797773963264 |
|---|---|
| author | Yuhang Xiao Jinming Tang Desong Yang Baihua Zhang Jie Wu Zhining Wu Qianjin Liao Hui Wang Wenxiang Wang Min Su |
| author_facet | Yuhang Xiao Jinming Tang Desong Yang Baihua Zhang Jie Wu Zhining Wu Qianjin Liao Hui Wang Wenxiang Wang Min Su |
| author_sort | Yuhang Xiao |
| collection | DOAJ |
| description | Abstract Introduction Esophageal squamous cell carcinoma (ESCC) represents a major malignancy with poor clinical outcomes. Long noncoding RNAs (lncRNAs) are known to regulate the development and progression of multiple cancers. However, how lncRNAs are involved in ESCC is currently undefined. Methods LIPH-4 levels in ESCC tissue specimens and cells were assessed by qRT-PCR. The biological function of LIPH-4 was examined in cell and animal studies, applying CCK-8, EdU, colony formation and flow cytometry assays as well as xenograft model experiments. The underlying mechanisms of action of LIPH-4 were explored through bioinformatics, luciferase reporter assay, RNA-immunoprecipitation assay and immunoblot. Results We identified a novel lncRNA, LIPH-4, which showed elevated amounts in ESCC tissues and positive correlations with increased tumor size and poor prognosis in ESCC patients. Functional studies showed that LIPH-4 promoted the growth, mediated cell cycle progression and inhibited apoptosis in ESCC cells in vitro, and promoted tumor growth in mice. In terms of mechanism, LIPH-4 could bind to miR-216b and act as a competing endogenous RNA (ceRNA) to induce the expression of miR-216’s target gene IGF2BP2. LIPH-4 played an oncogenic role in ESCC through the miR-216b/IGF2BP2 axis. Conclusions This study suggested that LIPH-4 functions as a novel oncogenic lncRNA by acting as a ceRNA for miR-216b to regulate IGF2BP2, indicating LIPH-4 likely constitutes a prognostic biomarker and therapeutic target in ESCC. |
| first_indexed | 2024-04-11T21:45:16Z |
| format | Article |
| id | doaj.art-d1fdd205c12b4bbfb4c35f13fd6d7bf0 |
| institution | Directory Open Access Journal |
| issn | 2050-7771 |
| language | English |
| last_indexed | 2024-04-11T21:45:16Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Biomarker Research |
| spelling | doaj.art-d1fdd205c12b4bbfb4c35f13fd6d7bf02022-12-22T04:01:26ZengBMCBiomarker Research2050-77712022-08-0110111210.1186/s40364-022-00408-xLong noncoding RNA LIPH-4 promotes esophageal squamous cell carcinoma progression by regulating the miR-216b/IGF2BP2 axisYuhang Xiao0Jinming Tang1Desong Yang2Baihua Zhang3Jie Wu4Zhining Wu5Qianjin Liao6Hui Wang7Wenxiang Wang8Min Su9Hunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal Carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal Carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal Carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal Carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal Carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal Carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityKey Laboratory of Translational Radiation Oncology, Department of Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal Carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal Carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityAbstract Introduction Esophageal squamous cell carcinoma (ESCC) represents a major malignancy with poor clinical outcomes. Long noncoding RNAs (lncRNAs) are known to regulate the development and progression of multiple cancers. However, how lncRNAs are involved in ESCC is currently undefined. Methods LIPH-4 levels in ESCC tissue specimens and cells were assessed by qRT-PCR. The biological function of LIPH-4 was examined in cell and animal studies, applying CCK-8, EdU, colony formation and flow cytometry assays as well as xenograft model experiments. The underlying mechanisms of action of LIPH-4 were explored through bioinformatics, luciferase reporter assay, RNA-immunoprecipitation assay and immunoblot. Results We identified a novel lncRNA, LIPH-4, which showed elevated amounts in ESCC tissues and positive correlations with increased tumor size and poor prognosis in ESCC patients. Functional studies showed that LIPH-4 promoted the growth, mediated cell cycle progression and inhibited apoptosis in ESCC cells in vitro, and promoted tumor growth in mice. In terms of mechanism, LIPH-4 could bind to miR-216b and act as a competing endogenous RNA (ceRNA) to induce the expression of miR-216’s target gene IGF2BP2. LIPH-4 played an oncogenic role in ESCC through the miR-216b/IGF2BP2 axis. Conclusions This study suggested that LIPH-4 functions as a novel oncogenic lncRNA by acting as a ceRNA for miR-216b to regulate IGF2BP2, indicating LIPH-4 likely constitutes a prognostic biomarker and therapeutic target in ESCC.https://doi.org/10.1186/s40364-022-00408-xEsophageal squamous cell carcinomaLong noncoding RNALIPH-4miR-216bIGF2BP2 |
| spellingShingle | Yuhang Xiao Jinming Tang Desong Yang Baihua Zhang Jie Wu Zhining Wu Qianjin Liao Hui Wang Wenxiang Wang Min Su Long noncoding RNA LIPH-4 promotes esophageal squamous cell carcinoma progression by regulating the miR-216b/IGF2BP2 axis Biomarker Research Esophageal squamous cell carcinoma Long noncoding RNA LIPH-4 miR-216b IGF2BP2 |
| title | Long noncoding RNA LIPH-4 promotes esophageal squamous cell carcinoma progression by regulating the miR-216b/IGF2BP2 axis |
| title_full | Long noncoding RNA LIPH-4 promotes esophageal squamous cell carcinoma progression by regulating the miR-216b/IGF2BP2 axis |
| title_fullStr | Long noncoding RNA LIPH-4 promotes esophageal squamous cell carcinoma progression by regulating the miR-216b/IGF2BP2 axis |
| title_full_unstemmed | Long noncoding RNA LIPH-4 promotes esophageal squamous cell carcinoma progression by regulating the miR-216b/IGF2BP2 axis |
| title_short | Long noncoding RNA LIPH-4 promotes esophageal squamous cell carcinoma progression by regulating the miR-216b/IGF2BP2 axis |
| title_sort | long noncoding rna liph 4 promotes esophageal squamous cell carcinoma progression by regulating the mir 216b igf2bp2 axis |
| topic | Esophageal squamous cell carcinoma Long noncoding RNA LIPH-4 miR-216b IGF2BP2 |
| url | https://doi.org/10.1186/s40364-022-00408-x |
| work_keys_str_mv | AT yuhangxiao longnoncodingrnaliph4promotesesophagealsquamouscellcarcinomaprogressionbyregulatingthemir216bigf2bp2axis AT jinmingtang longnoncodingrnaliph4promotesesophagealsquamouscellcarcinomaprogressionbyregulatingthemir216bigf2bp2axis AT desongyang longnoncodingrnaliph4promotesesophagealsquamouscellcarcinomaprogressionbyregulatingthemir216bigf2bp2axis AT baihuazhang longnoncodingrnaliph4promotesesophagealsquamouscellcarcinomaprogressionbyregulatingthemir216bigf2bp2axis AT jiewu longnoncodingrnaliph4promotesesophagealsquamouscellcarcinomaprogressionbyregulatingthemir216bigf2bp2axis AT zhiningwu longnoncodingrnaliph4promotesesophagealsquamouscellcarcinomaprogressionbyregulatingthemir216bigf2bp2axis AT qianjinliao longnoncodingrnaliph4promotesesophagealsquamouscellcarcinomaprogressionbyregulatingthemir216bigf2bp2axis AT huiwang longnoncodingrnaliph4promotesesophagealsquamouscellcarcinomaprogressionbyregulatingthemir216bigf2bp2axis AT wenxiangwang longnoncodingrnaliph4promotesesophagealsquamouscellcarcinomaprogressionbyregulatingthemir216bigf2bp2axis AT minsu longnoncodingrnaliph4promotesesophagealsquamouscellcarcinomaprogressionbyregulatingthemir216bigf2bp2axis |