Identification of Key <i>TRIM</i> Genes Involved in Response to <i>Pseudomonas aeruginosa</i> or <i>Chlamydia</i> spp. Infections in Human Cell Lines and in Mouse Organs

Identification of Key <i>TRIM</i> Genes Involved in Response to <i>Pseudomonas aeruginosa</i> or <i>Chlamydia</i> spp. Infections in Human Cell Lines and in Mouse Organs

Bacterial infections represent an unsolved problem today since bacteria can evade antibiotics and suppress the host’s immune response. A family of TRIM proteins is known to play a role in antiviral defense. However, the data on the involvement of the corresponding genes in the antibacterial response...

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Bibliographic Details
Main Authors: Ekaterina Stepanenko, Natalia Bondareva, Anna Sheremet, Elena Fedina, Alexei Tikhomirov, Tatiana Gerasimova, Daniil Poberezhniy, Irina Makarova, Vyacheslav Tarantul, Nailya Zigangirova, Valentina Nenasheva
Format: Article
Language:English
Published: MDPI AG 2023-08-01
Series:International Journal of Molecular Sciences
Subjects:
<i>TRIM</i> family genes
innate immune response
<i>Pseudomonas aeruginosa</i>
<i>Chlamydia</i> spp.
Online Access:https://www.mdpi.com/1422-0067/24/17/13290
Description
Summary:Bacterial infections represent an unsolved problem today since bacteria can evade antibiotics and suppress the host’s immune response. A family of TRIM proteins is known to play a role in antiviral defense. However, the data on the involvement of the corresponding genes in the antibacterial response are limited. Here, we used RT-qPCR to profile the transcript levels of <i>TRIM</i> genes, as well as interferons and inflammatory genes, in human cell lines (in vitro) and in mice (in vivo) after bacterial infections caused by <i>Pseudomonas aeruginosa</i> and <i>Chlamydia</i> spp. As a result, the genes were identified that are involved in the overall immune response and associated primarily with inflammation in human cells and in mouse organs when infected with both pathogens (<i>TRIM7</i>, <i>8</i>, <i>14</i>, <i>16</i>, <i>17</i>, <i>18</i>, <i>19</i>, <i>20</i>, <i>21</i>, <i>47</i>, <i>68</i>). <i>TRIMs</i> specific to the infection (<i>TRIM59</i> for <i>P. aeruginosa</i>, <i>TRIM67</i> for <i>Chlamydia</i> spp.) were revealed. Our findings can serve as a basis for further, more detailed studies on the mechanisms of the immune response to <i>P. aeruginosa</i> and <i>Chlamydia</i> spp. Studying the interaction between bacterial pathogens and the immune system contributes to the search for new ways to successfully fight bacterial infections.
ISSN:1661-6596
1422-0067

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