Bioaugmentation of latex rubber sheet wastewater treatment with stimulated indigenous purple nonsulfur bacteria by fermented pineapple extract
Background: Treating latex rubber sheet wastewater often leads to the generation of a rotten-egg odor from toxic H2S. To increase the treatment efficiency and eliminate H2S, purple nonsulfur bacteria (PNSB), prepared by supplementing non-sterile rubber sheet wastewater (RAW) with fermented pineapple...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-07-01
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| Series: | Electronic Journal of Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S071734581400058X |
| _version_ | 1818302666654089216 |
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| author | Nastee Kornochalert Duangporn Kantachote Sumate Chaiprapat Somkiet Techkarnjanaruk |
| author_facet | Nastee Kornochalert Duangporn Kantachote Sumate Chaiprapat Somkiet Techkarnjanaruk |
| author_sort | Nastee Kornochalert |
| collection | DOAJ |
| description | Background: Treating latex rubber sheet wastewater often leads to the generation of a rotten-egg odor from toxic H2S. To increase the treatment efficiency and eliminate H2S, purple nonsulfur bacteria (PNSB), prepared by supplementing non-sterile rubber sheet wastewater (RAW) with fermented pineapple extract (FPE), were used to treat this wastewater under microaerobic light conditions. The following 3 independent variables: chemical oxygen demand (COD), initial pH and FPE dose were investigated using the Box–Behnken design to find optimal conditions for stimulating the growth of indigenous PNSB (PNSBsi).
Results: The addition of 2.0% FPE into RAW, which had a COD of 2000 mg L-1 and an initial pH of 7.0, significantly decreased oxidation reduction potential (ORP) value and stimulated PNSBsi to reach a maximum of 7.8 log cfu mL-1 within 2 d. Consequently, these PNSBsi, used as inoculants, were investigated for their ability to treat the wastewater under microaerobic light conditions. A central composite design was used to determine the optimal conditions for the wastewater treatment. These proved to be 7% PNSBsi, 0.8% FPE and 4 d retention time and this combination resulted in a reduction of 91% for COD, 75% for suspended solids, 61% for total sulfide while H2S was not detected. Results of abiotic control and treatment sets indicated that H2S was produced by heterotrophic bacteria and it was then effectively deactivated by PNSBsi.
Conclusions: The stimulation of PNSB growth by FPE under light condition was to lower ORP, and PNSBsi proved to be effective for treating the wastewater. |
| first_indexed | 2024-12-13T05:42:32Z |
| format | Article |
| id | doaj.art-d1fdd7db9c604b03908735f0a9e0086e |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-13T05:42:32Z |
| publishDate | 2014-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-d1fdd7db9c604b03908735f0a9e0086e2022-12-21T23:57:45ZengElsevierElectronic Journal of Biotechnology0717-34582014-07-0117417418210.1016/j.ejbt.2014.06.003Bioaugmentation of latex rubber sheet wastewater treatment with stimulated indigenous purple nonsulfur bacteria by fermented pineapple extractNastee Kornochalert0Duangporn Kantachote1Sumate Chaiprapat2Somkiet Techkarnjanaruk3Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, ThailandDepartment of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, ThailandCenter of Excellence on Hazardous Substance Management, Hat Yai, ThailandBiochemical Engineering and Pilot Plant Research and Development Unit, National Center for Genetics Engineering and Biotechnology, Bangkok, ThailandBackground: Treating latex rubber sheet wastewater often leads to the generation of a rotten-egg odor from toxic H2S. To increase the treatment efficiency and eliminate H2S, purple nonsulfur bacteria (PNSB), prepared by supplementing non-sterile rubber sheet wastewater (RAW) with fermented pineapple extract (FPE), were used to treat this wastewater under microaerobic light conditions. The following 3 independent variables: chemical oxygen demand (COD), initial pH and FPE dose were investigated using the Box–Behnken design to find optimal conditions for stimulating the growth of indigenous PNSB (PNSBsi). Results: The addition of 2.0% FPE into RAW, which had a COD of 2000 mg L-1 and an initial pH of 7.0, significantly decreased oxidation reduction potential (ORP) value and stimulated PNSBsi to reach a maximum of 7.8 log cfu mL-1 within 2 d. Consequently, these PNSBsi, used as inoculants, were investigated for their ability to treat the wastewater under microaerobic light conditions. A central composite design was used to determine the optimal conditions for the wastewater treatment. These proved to be 7% PNSBsi, 0.8% FPE and 4 d retention time and this combination resulted in a reduction of 91% for COD, 75% for suspended solids, 61% for total sulfide while H2S was not detected. Results of abiotic control and treatment sets indicated that H2S was produced by heterotrophic bacteria and it was then effectively deactivated by PNSBsi. Conclusions: The stimulation of PNSB growth by FPE under light condition was to lower ORP, and PNSBsi proved to be effective for treating the wastewater.http://www.sciencedirect.com/science/article/pii/S071734581400058XBioremediationFermented plant extractResponse surface methodologyRotten-egg odorRubber wastewater |
| spellingShingle | Nastee Kornochalert Duangporn Kantachote Sumate Chaiprapat Somkiet Techkarnjanaruk Bioaugmentation of latex rubber sheet wastewater treatment with stimulated indigenous purple nonsulfur bacteria by fermented pineapple extract Electronic Journal of Biotechnology Bioremediation Fermented plant extract Response surface methodology Rotten-egg odor Rubber wastewater |
| title | Bioaugmentation of latex rubber sheet wastewater treatment with stimulated indigenous purple nonsulfur bacteria by fermented pineapple extract |
| title_full | Bioaugmentation of latex rubber sheet wastewater treatment with stimulated indigenous purple nonsulfur bacteria by fermented pineapple extract |
| title_fullStr | Bioaugmentation of latex rubber sheet wastewater treatment with stimulated indigenous purple nonsulfur bacteria by fermented pineapple extract |
| title_full_unstemmed | Bioaugmentation of latex rubber sheet wastewater treatment with stimulated indigenous purple nonsulfur bacteria by fermented pineapple extract |
| title_short | Bioaugmentation of latex rubber sheet wastewater treatment with stimulated indigenous purple nonsulfur bacteria by fermented pineapple extract |
| title_sort | bioaugmentation of latex rubber sheet wastewater treatment with stimulated indigenous purple nonsulfur bacteria by fermented pineapple extract |
| topic | Bioremediation Fermented plant extract Response surface methodology Rotten-egg odor Rubber wastewater |
| url | http://www.sciencedirect.com/science/article/pii/S071734581400058X |
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