A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors
<p>Abstract</p> <p>Background</p> <p>Stable transgenesis is an undeniable key to understanding any genetic system. Retrovirus-based insertional strategies, which feature several technical challenges when they are used, are often limited to one particular species, and ev...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-09-01
|
| Series: | BMC Biotechnology |
| Online Access: | http://www.biomedcentral.com/1472-6750/9/81 |
| _version_ | 1818789914029850624 |
|---|---|
| author | Gandrillon Olivier Takahashi Yoshiko Kawakami Koichi Koszela Joanna Faure Claudine Viñuelas José Mejia-Pous Camila |
| author_facet | Gandrillon Olivier Takahashi Yoshiko Kawakami Koichi Koszela Joanna Faure Claudine Viñuelas José Mejia-Pous Camila |
| author_sort | Gandrillon Olivier |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Stable transgenesis is an undeniable key to understanding any genetic system. Retrovirus-based insertional strategies, which feature several technical challenges when they are used, are often limited to one particular species, and even sometimes to a particular cell type as the infection depends on certain cellular receptors. A universal-like system, which would allow both stable transgene expression independent of the cell type and an efficient sorting of transfected cells, is required when handling cellular models that are incompatible with retroviral strategies.</p> <p>Results</p> <p>We report here on the combination of a stable insertional transgenesis technique, based on the Tol2 transposon system together with the magnetic cell sorting (MACS) technique, which allows specific selection of cells carrying the transgene in an efficient, reliable and rapid way.</p> <p>Conclusion</p> <p>This new Tol2/MACS system leads to stable expression in a culture of primary chicken erythroid cells highly enriched in cells expressing the transgene of interest. This system could be used in a wide variety of vertebrate species.</p> |
| first_indexed | 2024-12-18T14:47:07Z |
| format | Article |
| id | doaj.art-d1ff9e0e64ed4149beb5c23668a2cf32 |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-12-18T14:47:07Z |
| publishDate | 2009-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-d1ff9e0e64ed4149beb5c23668a2cf322022-12-21T21:04:16ZengBMCBMC Biotechnology1472-67502009-09-01918110.1186/1472-6750-9-81A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitorsGandrillon OlivierTakahashi YoshikoKawakami KoichiKoszela JoannaFaure ClaudineViñuelas JoséMejia-Pous Camila<p>Abstract</p> <p>Background</p> <p>Stable transgenesis is an undeniable key to understanding any genetic system. Retrovirus-based insertional strategies, which feature several technical challenges when they are used, are often limited to one particular species, and even sometimes to a particular cell type as the infection depends on certain cellular receptors. A universal-like system, which would allow both stable transgene expression independent of the cell type and an efficient sorting of transfected cells, is required when handling cellular models that are incompatible with retroviral strategies.</p> <p>Results</p> <p>We report here on the combination of a stable insertional transgenesis technique, based on the Tol2 transposon system together with the magnetic cell sorting (MACS) technique, which allows specific selection of cells carrying the transgene in an efficient, reliable and rapid way.</p> <p>Conclusion</p> <p>This new Tol2/MACS system leads to stable expression in a culture of primary chicken erythroid cells highly enriched in cells expressing the transgene of interest. This system could be used in a wide variety of vertebrate species.</p>http://www.biomedcentral.com/1472-6750/9/81 |
| spellingShingle | Gandrillon Olivier Takahashi Yoshiko Kawakami Koichi Koszela Joanna Faure Claudine Viñuelas José Mejia-Pous Camila A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors BMC Biotechnology |
| title | A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors |
| title_full | A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors |
| title_fullStr | A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors |
| title_full_unstemmed | A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors |
| title_short | A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors |
| title_sort | combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors |
| url | http://www.biomedcentral.com/1472-6750/9/81 |
| work_keys_str_mv | AT gandrillonolivier acombinationoftransposableelementsandmagneticcellsortingprovidesaveryefficienttransgenesissystemforchickenprimaryerythroidprogenitors AT takahashiyoshiko acombinationoftransposableelementsandmagneticcellsortingprovidesaveryefficienttransgenesissystemforchickenprimaryerythroidprogenitors AT kawakamikoichi acombinationoftransposableelementsandmagneticcellsortingprovidesaveryefficienttransgenesissystemforchickenprimaryerythroidprogenitors AT koszelajoanna acombinationoftransposableelementsandmagneticcellsortingprovidesaveryefficienttransgenesissystemforchickenprimaryerythroidprogenitors AT faureclaudine acombinationoftransposableelementsandmagneticcellsortingprovidesaveryefficienttransgenesissystemforchickenprimaryerythroidprogenitors AT vinuelasjose acombinationoftransposableelementsandmagneticcellsortingprovidesaveryefficienttransgenesissystemforchickenprimaryerythroidprogenitors AT mejiapouscamila acombinationoftransposableelementsandmagneticcellsortingprovidesaveryefficienttransgenesissystemforchickenprimaryerythroidprogenitors AT gandrillonolivier combinationoftransposableelementsandmagneticcellsortingprovidesaveryefficienttransgenesissystemforchickenprimaryerythroidprogenitors AT takahashiyoshiko combinationoftransposableelementsandmagneticcellsortingprovidesaveryefficienttransgenesissystemforchickenprimaryerythroidprogenitors AT kawakamikoichi combinationoftransposableelementsandmagneticcellsortingprovidesaveryefficienttransgenesissystemforchickenprimaryerythroidprogenitors AT koszelajoanna combinationoftransposableelementsandmagneticcellsortingprovidesaveryefficienttransgenesissystemforchickenprimaryerythroidprogenitors AT faureclaudine combinationoftransposableelementsandmagneticcellsortingprovidesaveryefficienttransgenesissystemforchickenprimaryerythroidprogenitors AT vinuelasjose combinationoftransposableelementsandmagneticcellsortingprovidesaveryefficienttransgenesissystemforchickenprimaryerythroidprogenitors AT mejiapouscamila combinationoftransposableelementsandmagneticcellsortingprovidesaveryefficienttransgenesissystemforchickenprimaryerythroidprogenitors |