Expression analysis of genes responsible for serotonin signaling in the brain
To thoroughly understand the function and regulation of neurotransmitter systems in the brain, as well as the underlying disease mechanisms, it is important to comprehensively analyze the expression patterns of genes participating in such systems. Using functional annotated cDNA clones (FANTOM), we...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2005-08-01
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| Series: | Neurobiology of Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S096999610500029X |
| _version_ | 1818862608166420480 |
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| author | Manabu Ichikawa Yuko Okamura-Oho Rie Okunishi Mutsumi Kanamori Harukazu Suzuki Akishige Ritani Hiroaki Nitta Naomi Eguchi Yoshihiro Urade Yoshihide Hayashizaki |
| author_facet | Manabu Ichikawa Yuko Okamura-Oho Rie Okunishi Mutsumi Kanamori Harukazu Suzuki Akishige Ritani Hiroaki Nitta Naomi Eguchi Yoshihiro Urade Yoshihide Hayashizaki |
| author_sort | Manabu Ichikawa |
| collection | DOAJ |
| description | To thoroughly understand the function and regulation of neurotransmitter systems in the brain, as well as the underlying disease mechanisms, it is important to comprehensively analyze the expression patterns of genes participating in such systems. Using functional annotated cDNA clones (FANTOM), we examined the gene expression patterns of the serotonin neurotransmitter system, which is involved in psychiatric diseases such as depression. We chose 24 gene products and visualized their endogenous localizations using in situ hybridization (ISH). We were able to fine-tune an automated ISH method to obtain high-resolution cell-based figures within 24 h. We also measured the amounts of mRNAs with quantitative RT-PCR. The outline of the in situ gene expression pattern viewed under low magnification agreed with the results of the RT-PCR. In the high-resolution view obtained with ISH, we could document novel localizations of the several genes critically related to serotonin activity. |
| first_indexed | 2024-12-19T10:02:34Z |
| format | Article |
| id | doaj.art-d1ffb0bc35c74322af1c68051765bb08 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-19T10:02:34Z |
| publishDate | 2005-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-d1ffb0bc35c74322af1c68051765bb082022-12-21T20:26:37ZengElsevierNeurobiology of Disease1095-953X2005-08-01193378385Expression analysis of genes responsible for serotonin signaling in the brainManabu Ichikawa0Yuko Okamura-Oho1Rie Okunishi2Mutsumi Kanamori3Harukazu Suzuki4Akishige Ritani5Hiroaki Nitta6Naomi Eguchi7Yoshihiro Urade8Yoshihide Hayashizaki9Laboratory for Genome Exploration Research Group,RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan; Division of Genomic Information Resources, Science of Biological Supramolecular Systems,Graduate School of Integrated Science, Yokohama City University, JapanLaboratory for Genome Exploration Research Group,RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan; Corresponding author. Fax: +81 45 503 9216.Laboratory for Genome Exploration Research Group,RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan; Division of Genomic Information Resources, Science of Biological Supramolecular Systems,Graduate School of Integrated Science, Yokohama City University, JapanLaboratory for Genome Exploration Research Group,RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, JapanLaboratory for Genome Exploration Research Group,RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, JapanVENTANA Japan K K., JapanVENTANA Japan K K., JapanDepartment of Molecular Behavioral Biology, Osaka Bioscience Institute, JapanDepartment of Molecular Behavioral Biology, Osaka Bioscience Institute, JapanLaboratory for Genome Exploration Research Group,RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan; Division of Genomic Information Resources, Science of Biological Supramolecular Systems,Graduate School of Integrated Science, Yokohama City University, JapanTo thoroughly understand the function and regulation of neurotransmitter systems in the brain, as well as the underlying disease mechanisms, it is important to comprehensively analyze the expression patterns of genes participating in such systems. Using functional annotated cDNA clones (FANTOM), we examined the gene expression patterns of the serotonin neurotransmitter system, which is involved in psychiatric diseases such as depression. We chose 24 gene products and visualized their endogenous localizations using in situ hybridization (ISH). We were able to fine-tune an automated ISH method to obtain high-resolution cell-based figures within 24 h. We also measured the amounts of mRNAs with quantitative RT-PCR. The outline of the in situ gene expression pattern viewed under low magnification agreed with the results of the RT-PCR. In the high-resolution view obtained with ISH, we could document novel localizations of the several genes critically related to serotonin activity.http://www.sciencedirect.com/science/article/pii/S096999610500029XDepressionIn situ hybridizationRT-PCRSerotonin metabolismSerotonin receptorComprehensive study |
| spellingShingle | Manabu Ichikawa Yuko Okamura-Oho Rie Okunishi Mutsumi Kanamori Harukazu Suzuki Akishige Ritani Hiroaki Nitta Naomi Eguchi Yoshihiro Urade Yoshihide Hayashizaki Expression analysis of genes responsible for serotonin signaling in the brain Neurobiology of Disease Depression In situ hybridization RT-PCR Serotonin metabolism Serotonin receptor Comprehensive study |
| title | Expression analysis of genes responsible for serotonin signaling in the brain |
| title_full | Expression analysis of genes responsible for serotonin signaling in the brain |
| title_fullStr | Expression analysis of genes responsible for serotonin signaling in the brain |
| title_full_unstemmed | Expression analysis of genes responsible for serotonin signaling in the brain |
| title_short | Expression analysis of genes responsible for serotonin signaling in the brain |
| title_sort | expression analysis of genes responsible for serotonin signaling in the brain |
| topic | Depression In situ hybridization RT-PCR Serotonin metabolism Serotonin receptor Comprehensive study |
| url | http://www.sciencedirect.com/science/article/pii/S096999610500029X |
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