Effect of angiotensin(1-7) on heart function in an experimental rat model of obesity
Aim: Obesity is a risk factor for the development of cardiovascular diseases. Recently it was shown that overexpression of the Mas- receptor antagonist angiotensin(1-7) could prevent from diet- induced obesity. However, it remained unclear whether diet-induced obesity and angiotensin(1-7) overexpres...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-12-01
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| Series: | Frontiers in Physiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fphys.2015.00392/full |
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| author | Katja eBlanke Franziska eSchlegel Stefan eDhein Walter eRaasch Michael eBader Ingo eDähnert Aida eSalameh |
| author_facet | Katja eBlanke Franziska eSchlegel Stefan eDhein Walter eRaasch Michael eBader Ingo eDähnert Aida eSalameh |
| author_sort | Katja eBlanke |
| collection | DOAJ |
| description | Aim: Obesity is a risk factor for the development of cardiovascular diseases. Recently it was shown that overexpression of the Mas- receptor antagonist angiotensin(1-7) could prevent from diet- induced obesity. However, it remained unclear whether diet-induced obesity and angiotensin(1-7) overexpression might also have effects on the cardiovascular system in these rats.Methods: 23 male Sprague Dawley rats were fed with standard chow (SD+chow, n=5) or a cafeteria diet (SD+CD, n=6) for five months. To investigate the effect of angiotensin(1-7) transgenic rats, expressing an angiotensin(1-7)-producing fusion protein in testis were used. These transgenic rats also received a five month’s feeding period with either chow (TGR+chow, n=6) or cafeteria diet (TGR+CD, n=6), respectively. Hemodynamic measurements (pressure-volume loops) were carried out to assess cardiac function and blood pressure. Subsequently, hearts were explanted and investigated according to the Langendorff technique. Furthermore, cardiac remodeling in these animals was investigated histologically.Results: After five months cafeteria diet feeding rats showed a significantly increased body weight, which could be prevented in transgenic rats. However, there was no effect on cardiac performance after cafeteria diet in non-transgenic and transgenic rats. Moreover, overexpression of angiotensin(1-7) deteriorated cardiac contractility as indicated by impaired dp/dt. Furthermore, histological analysis revealed that cafeteria diet led to myocardial fibrosis in both, control and transgenic rats and this was not inhibited by an overproduction of angiotensin(1-7).Conclusion: These results indicate that an overexpression of circulating angiotensin(1-7) prevents a cafeteria diet-induced increase in body weight, but does not affect cardiac performance in this experimental rat model of obesity. Furthermore, overexpression of angiotensin(1-7) alone resulted in an impairment of cardiac function. |
| first_indexed | 2024-04-12T10:51:35Z |
| format | Article |
| id | doaj.art-d1ffb5427bed4d9bb944b80d31536944 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-12T10:51:35Z |
| publishDate | 2015-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-d1ffb5427bed4d9bb944b80d315369442022-12-22T03:36:14ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2015-12-01610.3389/fphys.2015.00392167716Effect of angiotensin(1-7) on heart function in an experimental rat model of obesityKatja eBlanke0Franziska eSchlegel1Stefan eDhein2Walter eRaasch3Michael eBader4Ingo eDähnert5Aida eSalameh6Heart Center LeipzigHeart Center LeipzigHeart Center LeipzigUniversity of LübeckMax-Delbrück Center for Molecular MedicineHeart Center LeipzigHeart Center LeipzigAim: Obesity is a risk factor for the development of cardiovascular diseases. Recently it was shown that overexpression of the Mas- receptor antagonist angiotensin(1-7) could prevent from diet- induced obesity. However, it remained unclear whether diet-induced obesity and angiotensin(1-7) overexpression might also have effects on the cardiovascular system in these rats.Methods: 23 male Sprague Dawley rats were fed with standard chow (SD+chow, n=5) or a cafeteria diet (SD+CD, n=6) for five months. To investigate the effect of angiotensin(1-7) transgenic rats, expressing an angiotensin(1-7)-producing fusion protein in testis were used. These transgenic rats also received a five month’s feeding period with either chow (TGR+chow, n=6) or cafeteria diet (TGR+CD, n=6), respectively. Hemodynamic measurements (pressure-volume loops) were carried out to assess cardiac function and blood pressure. Subsequently, hearts were explanted and investigated according to the Langendorff technique. Furthermore, cardiac remodeling in these animals was investigated histologically.Results: After five months cafeteria diet feeding rats showed a significantly increased body weight, which could be prevented in transgenic rats. However, there was no effect on cardiac performance after cafeteria diet in non-transgenic and transgenic rats. Moreover, overexpression of angiotensin(1-7) deteriorated cardiac contractility as indicated by impaired dp/dt. Furthermore, histological analysis revealed that cafeteria diet led to myocardial fibrosis in both, control and transgenic rats and this was not inhibited by an overproduction of angiotensin(1-7).Conclusion: These results indicate that an overexpression of circulating angiotensin(1-7) prevents a cafeteria diet-induced increase in body weight, but does not affect cardiac performance in this experimental rat model of obesity. Furthermore, overexpression of angiotensin(1-7) alone resulted in an impairment of cardiac function.http://journal.frontiersin.org/Journal/10.3389/fphys.2015.00392/fullHeartObesityTransgenic ratsCafeteria dietAngiotensin(1-7) |
| spellingShingle | Katja eBlanke Franziska eSchlegel Stefan eDhein Walter eRaasch Michael eBader Ingo eDähnert Aida eSalameh Effect of angiotensin(1-7) on heart function in an experimental rat model of obesity Frontiers in Physiology Heart Obesity Transgenic rats Cafeteria diet Angiotensin(1-7) |
| title | Effect of angiotensin(1-7) on heart function in an experimental rat model of obesity |
| title_full | Effect of angiotensin(1-7) on heart function in an experimental rat model of obesity |
| title_fullStr | Effect of angiotensin(1-7) on heart function in an experimental rat model of obesity |
| title_full_unstemmed | Effect of angiotensin(1-7) on heart function in an experimental rat model of obesity |
| title_short | Effect of angiotensin(1-7) on heart function in an experimental rat model of obesity |
| title_sort | effect of angiotensin 1 7 on heart function in an experimental rat model of obesity |
| topic | Heart Obesity Transgenic rats Cafeteria diet Angiotensin(1-7) |
| url | http://journal.frontiersin.org/Journal/10.3389/fphys.2015.00392/full |
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