The Association between TNF-alpha Gene Polymorphisms and Endometriosis in An Iranian Population
Background Tumor necrosis factor-alpha (TNF-α) is an important cytokine in acute inflammatory response to infective factors. Based on investigation in different populations, it is thought that this response increases in patients with endometrio- sis due to the presence of cytokines such as TNF-α....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2019-04-01
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| Series: | International Journal of Fertility and Sterility |
| Subjects: | |
| Online Access: | http://www.ijfs.ir/article_45516_f43a7d4326e31ea1ba1f2ba2bee5d07c.pdf |
| _version_ | 1811295273656254464 |
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| author | Babak Babaabasi Ali Ahani Faegheh Sadeghi Haniyeh Bashizade-Fakhar Hamid Reza Khorram Khorshid |
| author_facet | Babak Babaabasi Ali Ahani Faegheh Sadeghi Haniyeh Bashizade-Fakhar Hamid Reza Khorram Khorshid |
| author_sort | Babak Babaabasi |
| collection | DOAJ |
| description | Background Tumor necrosis factor-alpha (TNF-α) is an important cytokine in acute inflammatory response to infective factors. Based on investigation in different populations, it is thought that this response increases in patients with endometrio- sis due to the presence of cytokines such as TNF-α. This study aimed to examine the association of four TNF-α polymor- phisms, namely -238G/A, -308G/A, -857C/T and -863C/A, with susceptibility to endometriosis in an Iranian population. Materials and Methods We recruited 150 women with endometriosis and 150 women without endometriosis in this case-control study and collected 4 ml of blood from all subjects. After DNA extraction, the polymorphisms were geno- typed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The allele frequency of TNF-α -863C/A in the case and control groups showed a significant difference [odds ratios (OR)=0.64, 95% confidence interval (CI)=0.41-0.99, P=0.047] but the result is not significant when Adjust- ing for multiple testing (P=0.188). No significant difference in the allele frequencies of -238G/A (OR=1.07, 95% CI=0.51-2.25, P=0.862), -308G/A (OR=0.79, 95% CI=0.43-1.45, P=0.438) and -857C/T (OR=1.03, 95% CI=0.66- 1.61, P=0.887) was observed. We adjusted all four polymorphism genotypes by age and body mass index (BMI), however, no significant difference was detected. There was an association between the case and control and BMI when adjusting by age (OR=1.082, 95% CI=1.009-1.162, P=0.028). Conclusion For the first time the association of the four polymorphisms in the promoter region of the TNF-α gene with endometriosis has been conducted in women of Iranian origin. The present research reveals the -863 A allele may play a role in incidence of endometriosis among Iranian women. Development of endometriosis among those people with -863 A allele seems low. According to the results, the current study indicates that there might be a correlation between BMI and progression of endometriosis. |
| first_indexed | 2024-04-13T05:30:39Z |
| format | Article |
| id | doaj.art-d2021e811e214653bd0049ac31866fc5 |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-04-13T05:30:39Z |
| publishDate | 2019-04-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-d2021e811e214653bd0049ac31866fc52022-12-22T03:00:27ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782019-04-0113161110.22074/ijfs.2019.554245516The Association between TNF-alpha Gene Polymorphisms and Endometriosis in An Iranian PopulationBabak Babaabasi0Ali Ahani1Faegheh Sadeghi2Haniyeh Bashizade-Fakhar3Hamid Reza Khorram Khorshid4Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran,Mendel Medical Genetics Laboratory, Tehran, Iran4Department of Molecular Biology, Ahar Branch, Islamic Azad University, Ahar, Iran5Department of Laboratory Science, Chalous Branch, Islamic Azad University, Chalous, Iran6Genetic Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, IranBackground Tumor necrosis factor-alpha (TNF-α) is an important cytokine in acute inflammatory response to infective factors. Based on investigation in different populations, it is thought that this response increases in patients with endometrio- sis due to the presence of cytokines such as TNF-α. This study aimed to examine the association of four TNF-α polymor- phisms, namely -238G/A, -308G/A, -857C/T and -863C/A, with susceptibility to endometriosis in an Iranian population. Materials and Methods We recruited 150 women with endometriosis and 150 women without endometriosis in this case-control study and collected 4 ml of blood from all subjects. After DNA extraction, the polymorphisms were geno- typed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The allele frequency of TNF-α -863C/A in the case and control groups showed a significant difference [odds ratios (OR)=0.64, 95% confidence interval (CI)=0.41-0.99, P=0.047] but the result is not significant when Adjust- ing for multiple testing (P=0.188). No significant difference in the allele frequencies of -238G/A (OR=1.07, 95% CI=0.51-2.25, P=0.862), -308G/A (OR=0.79, 95% CI=0.43-1.45, P=0.438) and -857C/T (OR=1.03, 95% CI=0.66- 1.61, P=0.887) was observed. We adjusted all four polymorphism genotypes by age and body mass index (BMI), however, no significant difference was detected. There was an association between the case and control and BMI when adjusting by age (OR=1.082, 95% CI=1.009-1.162, P=0.028). Conclusion For the first time the association of the four polymorphisms in the promoter region of the TNF-α gene with endometriosis has been conducted in women of Iranian origin. The present research reveals the -863 A allele may play a role in incidence of endometriosis among Iranian women. Development of endometriosis among those people with -863 A allele seems low. According to the results, the current study indicates that there might be a correlation between BMI and progression of endometriosis.http://www.ijfs.ir/article_45516_f43a7d4326e31ea1ba1f2ba2bee5d07c.pdfbody mass indexendometriosispolymorphismsrestriction fragment length polymorphism |
| spellingShingle | Babak Babaabasi Ali Ahani Faegheh Sadeghi Haniyeh Bashizade-Fakhar Hamid Reza Khorram Khorshid The Association between TNF-alpha Gene Polymorphisms and Endometriosis in An Iranian Population International Journal of Fertility and Sterility body mass index endometriosis polymorphisms restriction fragment length polymorphism |
| title | The Association between TNF-alpha Gene Polymorphisms and Endometriosis in An Iranian Population |
| title_full | The Association between TNF-alpha Gene Polymorphisms and Endometriosis in An Iranian Population |
| title_fullStr | The Association between TNF-alpha Gene Polymorphisms and Endometriosis in An Iranian Population |
| title_full_unstemmed | The Association between TNF-alpha Gene Polymorphisms and Endometriosis in An Iranian Population |
| title_short | The Association between TNF-alpha Gene Polymorphisms and Endometriosis in An Iranian Population |
| title_sort | association between tnf alpha gene polymorphisms and endometriosis in an iranian population |
| topic | body mass index endometriosis polymorphisms restriction fragment length polymorphism |
| url | http://www.ijfs.ir/article_45516_f43a7d4326e31ea1ba1f2ba2bee5d07c.pdf |
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