Patient-Specific Variables Determine the Extent of Cellular Senescence Biomarkers in Ovarian Tumors In Vivo
The mechanisms and clinical significance of the cellular senescence of tumor cells are a matter of ongoing debate. Recently, the triggers and molecular events underlying spontaneous, replicative senescence of primary epithelial ovarian cancer cells were characterized. In this study, we reanalyzed tu...
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MDPI AG
2021-03-01
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| author | Paweł Uruski Justyna Mikuła-Pietrasik Eryk Naumowicz Kamila Kaźmierczak Andrey N. Gaiday Jan Królak Błażej Nowakowski Rafał Moszyński Andrzej Tykarski Krzysztof Książek |
| author_facet | Paweł Uruski Justyna Mikuła-Pietrasik Eryk Naumowicz Kamila Kaźmierczak Andrey N. Gaiday Jan Królak Błażej Nowakowski Rafał Moszyński Andrzej Tykarski Krzysztof Książek |
| author_sort | Paweł Uruski |
| collection | DOAJ |
| description | The mechanisms and clinical significance of the cellular senescence of tumor cells are a matter of ongoing debate. Recently, the triggers and molecular events underlying spontaneous, replicative senescence of primary epithelial ovarian cancer cells were characterized. In this study, we reanalyzed tumors obtained from ovarian cancer patients with respect to the expression of the senescence biomarkers SA-β-Gal and γ-H2A.X and the proliferative antigen Ki67. The results showed that the tumors displayed strong heterogeneity with respect to the expression of analyzed markers. The expression of SA-β-Gal and γ-H2A.X in the oldest patients (61–85 y.o.) was significantly higher than in the younger age groups. Conversely, the area of Ki67-positive cancer cells was greater in younger individuals. At the same time, there was a positive correlation between SA-β-Gal expression and calendar age in FIGO III–IV and malignant ascites-positive patients. The γ-H2A.X positively correlated with age in the whole group, FIGO III–IV, and ascites-positive patients. Ki67 levels correlated negatively with the age of patients among those same groups. Collectively, our study indicated that organismal aging may determine the development of the senescence phenotype in ovarian tumors, particularly in patients with advanced disease and those accumulating malignant ascites. |
| first_indexed | 2024-03-10T12:56:32Z |
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| language | English |
| last_indexed | 2024-03-10T12:56:32Z |
| publishDate | 2021-03-01 |
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| series | Biomedicines |
| spelling | doaj.art-d20365f4865341f687620c0b90fb47e92023-11-21T11:55:05ZengMDPI AGBiomedicines2227-90592021-03-019433010.3390/biomedicines9040330Patient-Specific Variables Determine the Extent of Cellular Senescence Biomarkers in Ovarian Tumors In VivoPaweł Uruski0Justyna Mikuła-Pietrasik1Eryk Naumowicz2Kamila Kaźmierczak3Andrey N. Gaiday4Jan Królak5Błażej Nowakowski6Rafał Moszyński7Andrzej Tykarski8Krzysztof Książek9Department of Hypertensiology, Poznań University of Medical Sciences, 61-848 Poznań, PolandDepartment of Pathophysiology of Ageing and Civilization Diseases, Poznań University of Medical Sciences, 61-848 Poznań, PolandGeneral Surgery Ward, Medical Centre HCP, 61-485 Poznan, PolandThe Greater Poland Cancer Center, Department of Surgical, Oncological, and Endoscopic Gynecology, 61-866 Poznań, PolandDepartment of Obstetrics and Gynecology, West Kazakhstan Marat Ospanov Medical University, Aktobe 030008, KazakhstanDepartment of Hypertensiology, Poznań University of Medical Sciences, 61-848 Poznań, PolandThe Greater Poland Cancer Center, Department of Surgical, Oncological, and Endoscopic Gynecology, 61-866 Poznań, PolandDivision of Gynecological Surgery, Poznan University of Medical Sciences, 60-535 Poznan, PolandDepartment of Hypertensiology, Poznań University of Medical Sciences, 61-848 Poznań, PolandDepartment of Pathophysiology of Ageing and Civilization Diseases, Poznań University of Medical Sciences, 61-848 Poznań, PolandThe mechanisms and clinical significance of the cellular senescence of tumor cells are a matter of ongoing debate. Recently, the triggers and molecular events underlying spontaneous, replicative senescence of primary epithelial ovarian cancer cells were characterized. In this study, we reanalyzed tumors obtained from ovarian cancer patients with respect to the expression of the senescence biomarkers SA-β-Gal and γ-H2A.X and the proliferative antigen Ki67. The results showed that the tumors displayed strong heterogeneity with respect to the expression of analyzed markers. The expression of SA-β-Gal and γ-H2A.X in the oldest patients (61–85 y.o.) was significantly higher than in the younger age groups. Conversely, the area of Ki67-positive cancer cells was greater in younger individuals. At the same time, there was a positive correlation between SA-β-Gal expression and calendar age in FIGO III–IV and malignant ascites-positive patients. The γ-H2A.X positively correlated with age in the whole group, FIGO III–IV, and ascites-positive patients. Ki67 levels correlated negatively with the age of patients among those same groups. Collectively, our study indicated that organismal aging may determine the development of the senescence phenotype in ovarian tumors, particularly in patients with advanced disease and those accumulating malignant ascites.https://www.mdpi.com/2227-9059/9/4/330agingbiomarkerscellular senescenceFIGOmalignant ascitesovarian cancer |
| spellingShingle | Paweł Uruski Justyna Mikuła-Pietrasik Eryk Naumowicz Kamila Kaźmierczak Andrey N. Gaiday Jan Królak Błażej Nowakowski Rafał Moszyński Andrzej Tykarski Krzysztof Książek Patient-Specific Variables Determine the Extent of Cellular Senescence Biomarkers in Ovarian Tumors In Vivo Biomedicines aging biomarkers cellular senescence FIGO malignant ascites ovarian cancer |
| title | Patient-Specific Variables Determine the Extent of Cellular Senescence Biomarkers in Ovarian Tumors In Vivo |
| title_full | Patient-Specific Variables Determine the Extent of Cellular Senescence Biomarkers in Ovarian Tumors In Vivo |
| title_fullStr | Patient-Specific Variables Determine the Extent of Cellular Senescence Biomarkers in Ovarian Tumors In Vivo |
| title_full_unstemmed | Patient-Specific Variables Determine the Extent of Cellular Senescence Biomarkers in Ovarian Tumors In Vivo |
| title_short | Patient-Specific Variables Determine the Extent of Cellular Senescence Biomarkers in Ovarian Tumors In Vivo |
| title_sort | patient specific variables determine the extent of cellular senescence biomarkers in ovarian tumors in vivo |
| topic | aging biomarkers cellular senescence FIGO malignant ascites ovarian cancer |
| url | https://www.mdpi.com/2227-9059/9/4/330 |
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