Glucose-6-phosphate Isomerase as a Marker of a Fertility Restorer Gene in Rape - Short Communication
In breeding of winter rape (Brassica napus L. var. napus) using the OGU-INRA system, based on cytoplasmic male sterility (CMS), it is necessary to distinguish pollen-sterile lines, carrying CMS factors and the recessive fertility restorer alleles rfrf, from fertile lines, carrying at least one domin...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2003-12-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-200304-0006_glucose-6-phosphate-isomerase-as-a-marker-of-a-fertility-restorer-gene-in-rape-short-communication.php |
| _version_ | 1797899567469953024 |
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| author | J. Horáček M. Ačanová |
| author_facet | J. Horáček M. Ačanová |
| author_sort | J. Horáček |
| collection | DOAJ |
| description | In breeding of winter rape (Brassica napus L. var. napus) using the OGU-INRA system, based on cytoplasmic male sterility (CMS), it is necessary to distinguish pollen-sterile lines, carrying CMS factors and the recessive fertility restorer alleles rfrf, from fertile lines, carrying at least one dominant restorer gene allele (Rfrf or RfRf). To grow plants till the flowering stage takes much time. The method was therefore modified using isozyme markers of glucose-6-phosphate isomerase (PGI) to distinguish male sterile (MS) from male-fertile lines in early stages. Since the restorer gene is tightly linked to the markers and the PGI isozymes can be distinguished by electrophoresis, the markers can be used to identify MS rape plants in early stages. Also, homozygous and heterozygous fertility-restored plants can be separated this way. In our work we tried to optimise the distinction of pollen-fertile and pollen-sterile rape plants with PGI isozyme markers, using vertical polyacrylamide gel electrophoresis (native-PAGE). The method will be used for the breeding of rapeseed with the OGU-INRA system. |
| first_indexed | 2024-04-10T08:32:01Z |
| format | Article |
| id | doaj.art-d2079ea9d2264291a4c7257948e2291e |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:32:01Z |
| publishDate | 2003-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-d2079ea9d2264291a4c7257948e2291e2023-02-23T03:28:57ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252003-12-0139413013310.17221/3731-CJGPBcjg-200304-0006Glucose-6-phosphate Isomerase as a Marker of a Fertility Restorer Gene in Rape - Short CommunicationJ. Horáček0M. Ačanová1AGRITEC, Ltd., Šumperk, Czech RepublicAGRITEC, Ltd., Šumperk, Czech RepublicIn breeding of winter rape (Brassica napus L. var. napus) using the OGU-INRA system, based on cytoplasmic male sterility (CMS), it is necessary to distinguish pollen-sterile lines, carrying CMS factors and the recessive fertility restorer alleles rfrf, from fertile lines, carrying at least one dominant restorer gene allele (Rfrf or RfRf). To grow plants till the flowering stage takes much time. The method was therefore modified using isozyme markers of glucose-6-phosphate isomerase (PGI) to distinguish male sterile (MS) from male-fertile lines in early stages. Since the restorer gene is tightly linked to the markers and the PGI isozymes can be distinguished by electrophoresis, the markers can be used to identify MS rape plants in early stages. Also, homozygous and heterozygous fertility-restored plants can be separated this way. In our work we tried to optimise the distinction of pollen-fertile and pollen-sterile rape plants with PGI isozyme markers, using vertical polyacrylamide gel electrophoresis (native-PAGE). The method will be used for the breeding of rapeseed with the OGU-INRA system.https://cjgpb.agriculturejournals.cz/artkey/cjg-200304-0006_glucose-6-phosphate-isomerase-as-a-marker-of-a-fertility-restorer-gene-in-rape-short-communication.phpbrassica napuswinter rapeogura cytoplasmic male-sterility restorer geneisozymepgi (glucose-6-phosphate isomerase) |
| spellingShingle | J. Horáček M. Ačanová Glucose-6-phosphate Isomerase as a Marker of a Fertility Restorer Gene in Rape - Short Communication Czech Journal of Genetics and Plant Breeding brassica napus winter rape ogura cytoplasmic male-sterility restorer gene isozyme pgi (glucose-6-phosphate isomerase) |
| title | Glucose-6-phosphate Isomerase as a Marker of a Fertility Restorer Gene in Rape - Short Communication |
| title_full | Glucose-6-phosphate Isomerase as a Marker of a Fertility Restorer Gene in Rape - Short Communication |
| title_fullStr | Glucose-6-phosphate Isomerase as a Marker of a Fertility Restorer Gene in Rape - Short Communication |
| title_full_unstemmed | Glucose-6-phosphate Isomerase as a Marker of a Fertility Restorer Gene in Rape - Short Communication |
| title_short | Glucose-6-phosphate Isomerase as a Marker of a Fertility Restorer Gene in Rape - Short Communication |
| title_sort | glucose 6 phosphate isomerase as a marker of a fertility restorer gene in rape short communication |
| topic | brassica napus winter rape ogura cytoplasmic male-sterility restorer gene isozyme pgi (glucose-6-phosphate isomerase) |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-200304-0006_glucose-6-phosphate-isomerase-as-a-marker-of-a-fertility-restorer-gene-in-rape-short-communication.php |
| work_keys_str_mv | AT jhoracek glucose6phosphateisomeraseasamarkerofafertilityrestorergeneinrapeshortcommunication AT macanova glucose6phosphateisomeraseasamarkerofafertilityrestorergeneinrapeshortcommunication |