Chitosan – Polyphosphate nanoparticles for a targeted drug release at the absorption membrane
The aim of this study was to develop nanoparticles (NPs) providing a targeted drug release directly on the epithelium of the intestinal mucosa.NPs were prepared via ionic gelation between cationic chitosan (Cs) and anionic polyphosphate (PP). The resulting NPs were characterized by their size, polyd...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022018655 |
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| author | Ahmad Saleh Zeynep Burcu Akkuş-Dağdeviren Julian David Friedl Patrick Knoll Andreas Bernkop-Schnürch |
| author_facet | Ahmad Saleh Zeynep Burcu Akkuş-Dağdeviren Julian David Friedl Patrick Knoll Andreas Bernkop-Schnürch |
| author_sort | Ahmad Saleh |
| collection | DOAJ |
| description | The aim of this study was to develop nanoparticles (NPs) providing a targeted drug release directly on the epithelium of the intestinal mucosa.NPs were prepared via ionic gelation between cationic chitosan (Cs) and anionic polyphosphate (PP). The resulting NPs were characterized by their size, polydispersity index (PDI) and zeta potential. Isolated and cell-associated intestinal alkaline phosphatase (IAP) was employed to trigger polyphosphate cleavage in Cs-PP NPs which was quantified via malachite green assay. In parallel, the shift in zeta potential was determined. In-vitro drug release studies were performed in Franz diffusion cells with Cs-PP NPs containing rhodamine 123 as model active ingredient. Furthermore, cytotoxicity of Cs-PP NPs was assessed via resazurin assay on Caco-2 cells as well as via hemolysis assay on red blood cells.Cs-PP NPs exhibited an average size of 144.17 ± 10.95 nm and zeta potential of -12.6 ± 0.50 mV. The encapsulation efficiency of rhodamine 123 by Cs-PP NPs was 86.8%. After incubation with isolated IAP for 3 h the polyphosphate of Cs-PP NPs was cleaved to monophosphate and zeta potential raised up to -2.3 ± 0.30 mV. Cs-PP NPs showed a non-toxic profile. Within 3 h, 62.0 ± 10.8% and 14.1 ± 2.2% of total rhodamine 123 was released from Cs-PP NPs upon incubation with isolated as well as porcine intestine derived intestinal alkaline phosphatase (IAP), respectively.According to these results, Cs-PP NPs are promising drug delivery systems to enable a drug targeted release at the absorption membrane. |
| first_indexed | 2024-04-11T09:10:50Z |
| format | Article |
| id | doaj.art-d209a2b96a334d54bd1587672259809e |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-11T09:10:50Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-d209a2b96a334d54bd1587672259809e2022-12-22T04:32:31ZengElsevierHeliyon2405-84402022-09-0189e10577Chitosan – Polyphosphate nanoparticles for a targeted drug release at the absorption membraneAhmad Saleh0Zeynep Burcu Akkuş-Dağdeviren1Julian David Friedl2Patrick Knoll3Andreas Bernkop-Schnürch4Center for Chemistry and Biomedicine, Department of Pharmaceutical Technology, Institute of Pharmacy, University of Innsbruck, Innrain 80/82, 6020 Innsbruck, Austria; Department of Pharmacy, Universitas Mandala Waluya, A.H.Nasution, Kendari 93231, Southeast Sulawesi, IndonesiaCenter for Chemistry and Biomedicine, Department of Pharmaceutical Technology, Institute of Pharmacy, University of Innsbruck, Innrain 80/82, 6020 Innsbruck, AustriaCenter for Chemistry and Biomedicine, Department of Pharmaceutical Technology, Institute of Pharmacy, University of Innsbruck, Innrain 80/82, 6020 Innsbruck, AustriaCenter for Chemistry and Biomedicine, Department of Pharmaceutical Technology, Institute of Pharmacy, University of Innsbruck, Innrain 80/82, 6020 Innsbruck, AustriaCenter for Chemistry and Biomedicine, Department of Pharmaceutical Technology, Institute of Pharmacy, University of Innsbruck, Innrain 80/82, 6020 Innsbruck, Austria; Corresponding author.The aim of this study was to develop nanoparticles (NPs) providing a targeted drug release directly on the epithelium of the intestinal mucosa.NPs were prepared via ionic gelation between cationic chitosan (Cs) and anionic polyphosphate (PP). The resulting NPs were characterized by their size, polydispersity index (PDI) and zeta potential. Isolated and cell-associated intestinal alkaline phosphatase (IAP) was employed to trigger polyphosphate cleavage in Cs-PP NPs which was quantified via malachite green assay. In parallel, the shift in zeta potential was determined. In-vitro drug release studies were performed in Franz diffusion cells with Cs-PP NPs containing rhodamine 123 as model active ingredient. Furthermore, cytotoxicity of Cs-PP NPs was assessed via resazurin assay on Caco-2 cells as well as via hemolysis assay on red blood cells.Cs-PP NPs exhibited an average size of 144.17 ± 10.95 nm and zeta potential of -12.6 ± 0.50 mV. The encapsulation efficiency of rhodamine 123 by Cs-PP NPs was 86.8%. After incubation with isolated IAP for 3 h the polyphosphate of Cs-PP NPs was cleaved to monophosphate and zeta potential raised up to -2.3 ± 0.30 mV. Cs-PP NPs showed a non-toxic profile. Within 3 h, 62.0 ± 10.8% and 14.1 ± 2.2% of total rhodamine 123 was released from Cs-PP NPs upon incubation with isolated as well as porcine intestine derived intestinal alkaline phosphatase (IAP), respectively.According to these results, Cs-PP NPs are promising drug delivery systems to enable a drug targeted release at the absorption membrane.http://www.sciencedirect.com/science/article/pii/S2405844022018655ChitosanPolyphosphatePolymeric nanoparticlesDrug deliveryTargeted releaseAlkaline phosphatase |
| spellingShingle | Ahmad Saleh Zeynep Burcu Akkuş-Dağdeviren Julian David Friedl Patrick Knoll Andreas Bernkop-Schnürch Chitosan – Polyphosphate nanoparticles for a targeted drug release at the absorption membrane Heliyon Chitosan Polyphosphate Polymeric nanoparticles Drug delivery Targeted release Alkaline phosphatase |
| title | Chitosan – Polyphosphate nanoparticles for a targeted drug release at the absorption membrane |
| title_full | Chitosan – Polyphosphate nanoparticles for a targeted drug release at the absorption membrane |
| title_fullStr | Chitosan – Polyphosphate nanoparticles for a targeted drug release at the absorption membrane |
| title_full_unstemmed | Chitosan – Polyphosphate nanoparticles for a targeted drug release at the absorption membrane |
| title_short | Chitosan – Polyphosphate nanoparticles for a targeted drug release at the absorption membrane |
| title_sort | chitosan polyphosphate nanoparticles for a targeted drug release at the absorption membrane |
| topic | Chitosan Polyphosphate Polymeric nanoparticles Drug delivery Targeted release Alkaline phosphatase |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022018655 |
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