Electrospun 5-Chloro-7-iodo-8-hydroxyquinoline (Clioquinol)-Containing Poly(3-hydroxybutyrate)/Polyvinylpyrrolidone Antifungal Materials Prospective as Active Dressings against Esca
Esca is a grapevine disease known for centuries which pertains to the group of so-called vine trunk diseases. <i>Phaeomoniella chlamydospora (P. chlamydospora)</i> and <i>Phaeoacremonium aleophilum (P. aleophilum)</i> are the two main fungal pathogens associated with esca. No...
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MDPI AG
2022-01-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/3/367 |
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| author | Milena Ignatova Nasko Nachev Mariya Spasova Nevena Manolova Iliya Rashkov Mladen Naydenov |
| author_facet | Milena Ignatova Nasko Nachev Mariya Spasova Nevena Manolova Iliya Rashkov Mladen Naydenov |
| author_sort | Milena Ignatova |
| collection | DOAJ |
| description | Esca is a grapevine disease known for centuries which pertains to the group of so-called vine trunk diseases. <i>Phaeomoniella chlamydospora (P. chlamydospora)</i> and <i>Phaeoacremonium aleophilum (P. aleophilum)</i> are the two main fungal pathogens associated with esca. Novel fibrous materials with antifungal properties based on poly(3-hydroxybutyrate) (PHB), polyvinylpyrrolidone (PVP) and 5-chloro-7-iodo-8-hydroxyquinoline (clioquinol, CQ) were developed. One-pot electrospinning (“<i>in</i>” strategy) or electrospinning in conjunction with electrospraying (“<i>on</i>” strategy) were applied to obtain the materials. The materials’ morphology and their surface chemical composition were examined using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). CQ incorporated in the bulk of the fibers or in PVP particles deposited on the fibers was in the amorphous phase, which was confirmed by differential scanning calorimetry (DSC) and X-ray diffraction analysis (XRD). The in vitro release of CQ depended on the composition of the electrospun materials and on their design. The performed microbiological screening revealed that, unlike the non-loaded mats, the fibrous mats loaded with CQ were effective in inhibiting the growth of the pathogenic <i>P. chlamydospora</i> and <i>P. aleophilum</i> fungi. Therefore, the created materials are promising as active dressings for grapevine protection against esca. |
| first_indexed | 2024-03-09T23:17:57Z |
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| language | English |
| last_indexed | 2024-03-09T23:17:57Z |
| publishDate | 2022-01-01 |
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| series | Polymers |
| spelling | doaj.art-d20e192af300463482a26e96aed735ff2023-11-23T17:32:40ZengMDPI AGPolymers2073-43602022-01-0114336710.3390/polym14030367Electrospun 5-Chloro-7-iodo-8-hydroxyquinoline (Clioquinol)-Containing Poly(3-hydroxybutyrate)/Polyvinylpyrrolidone Antifungal Materials Prospective as Active Dressings against EscaMilena Ignatova0Nasko Nachev1Mariya Spasova2Nevena Manolova3Iliya Rashkov4Mladen Naydenov5Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St, Bl. 103A, BG-1113 Sofia, BulgariaLaboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St, Bl. 103A, BG-1113 Sofia, BulgariaLaboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St, Bl. 103A, BG-1113 Sofia, BulgariaLaboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St, Bl. 103A, BG-1113 Sofia, BulgariaLaboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St, Bl. 103A, BG-1113 Sofia, BulgariaDepartment of Microbiology, Agricultural University, BG-4000 Plovdiv, BulgariaEsca is a grapevine disease known for centuries which pertains to the group of so-called vine trunk diseases. <i>Phaeomoniella chlamydospora (P. chlamydospora)</i> and <i>Phaeoacremonium aleophilum (P. aleophilum)</i> are the two main fungal pathogens associated with esca. Novel fibrous materials with antifungal properties based on poly(3-hydroxybutyrate) (PHB), polyvinylpyrrolidone (PVP) and 5-chloro-7-iodo-8-hydroxyquinoline (clioquinol, CQ) were developed. One-pot electrospinning (“<i>in</i>” strategy) or electrospinning in conjunction with electrospraying (“<i>on</i>” strategy) were applied to obtain the materials. The materials’ morphology and their surface chemical composition were examined using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). CQ incorporated in the bulk of the fibers or in PVP particles deposited on the fibers was in the amorphous phase, which was confirmed by differential scanning calorimetry (DSC) and X-ray diffraction analysis (XRD). The in vitro release of CQ depended on the composition of the electrospun materials and on their design. The performed microbiological screening revealed that, unlike the non-loaded mats, the fibrous mats loaded with CQ were effective in inhibiting the growth of the pathogenic <i>P. chlamydospora</i> and <i>P. aleophilum</i> fungi. Therefore, the created materials are promising as active dressings for grapevine protection against esca.https://www.mdpi.com/2073-4360/14/3/3675-chloro-7-iodo-8-hydroxyquinoline (clioquinol)poly(3-hydroxybutyrate)polyvinylpyrrolidoneelectrospinningelectrosprayingantifungal activity |
| spellingShingle | Milena Ignatova Nasko Nachev Mariya Spasova Nevena Manolova Iliya Rashkov Mladen Naydenov Electrospun 5-Chloro-7-iodo-8-hydroxyquinoline (Clioquinol)-Containing Poly(3-hydroxybutyrate)/Polyvinylpyrrolidone Antifungal Materials Prospective as Active Dressings against Esca Polymers 5-chloro-7-iodo-8-hydroxyquinoline (clioquinol) poly(3-hydroxybutyrate) polyvinylpyrrolidone electrospinning electrospraying antifungal activity |
| title | Electrospun 5-Chloro-7-iodo-8-hydroxyquinoline (Clioquinol)-Containing Poly(3-hydroxybutyrate)/Polyvinylpyrrolidone Antifungal Materials Prospective as Active Dressings against Esca |
| title_full | Electrospun 5-Chloro-7-iodo-8-hydroxyquinoline (Clioquinol)-Containing Poly(3-hydroxybutyrate)/Polyvinylpyrrolidone Antifungal Materials Prospective as Active Dressings against Esca |
| title_fullStr | Electrospun 5-Chloro-7-iodo-8-hydroxyquinoline (Clioquinol)-Containing Poly(3-hydroxybutyrate)/Polyvinylpyrrolidone Antifungal Materials Prospective as Active Dressings against Esca |
| title_full_unstemmed | Electrospun 5-Chloro-7-iodo-8-hydroxyquinoline (Clioquinol)-Containing Poly(3-hydroxybutyrate)/Polyvinylpyrrolidone Antifungal Materials Prospective as Active Dressings against Esca |
| title_short | Electrospun 5-Chloro-7-iodo-8-hydroxyquinoline (Clioquinol)-Containing Poly(3-hydroxybutyrate)/Polyvinylpyrrolidone Antifungal Materials Prospective as Active Dressings against Esca |
| title_sort | electrospun 5 chloro 7 iodo 8 hydroxyquinoline clioquinol containing poly 3 hydroxybutyrate polyvinylpyrrolidone antifungal materials prospective as active dressings against esca |
| topic | 5-chloro-7-iodo-8-hydroxyquinoline (clioquinol) poly(3-hydroxybutyrate) polyvinylpyrrolidone electrospinning electrospraying antifungal activity |
| url | https://www.mdpi.com/2073-4360/14/3/367 |
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