Expression levels of th-2 and th-17 characteristic genes in healthy tissue versus periodontitis.
The T helper 17 (Th17) cells have been suggested to have osteoclast activating effects while T helper 2 (Th2) cells are considered to have an osteoprotective role in periodontitis. This study was to compare the markers of Th17 cells (RORC2 and IL-17 genes) with that of Th2 cells (IL-4 and GATA-3 gen...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2013-01-01
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| Series: | Frontiers in Dentistry |
| Subjects: | |
| Online Access: | https://jdt.tums.ac.ir/index.php/jdt/article/view/711 |
| _version_ | 1819225904325328896 |
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| author | Parichehr Behfarnia Reza Birang Seyed Sajad Pishva Mazdak Ghanjalikhani Hakemi Mitra Mohammadi Khorasani |
| author_facet | Parichehr Behfarnia Reza Birang Seyed Sajad Pishva Mazdak Ghanjalikhani Hakemi Mitra Mohammadi Khorasani |
| author_sort | Parichehr Behfarnia |
| collection | DOAJ |
| description | The T helper 17 (Th17) cells have been suggested to have osteoclast activating effects while T helper 2 (Th2) cells are considered to have an osteoprotective role in periodontitis. This study was to compare the markers of Th17 cells (RORC2 and IL-17 genes) with that of Th2 cells (IL-4 and GATA-3 genes) between healthy and periodontitis tissues.The samples were obtained from patients with periodontitis and healthy tissues. The mRNA expression levels of IL-17, RORC2, IL-4 and GATA-3 were measured in both groups using quantitative RT-PCR. The results were compared using SPSS 11.0. Correlation coefficient was analyzed by Spearman's rho test. Mann-Whitney was used to measure the difference between IL-17 and IL-4 as well as RORC2 and GATA-3.In periodontal lesions, the expression levels of all markers were significantly higher than the healthy tissue (p≤ 0.001). The results showed a significant increase in the number of markers of Th17 (RORC2 and IL-17 genes) compared to markers of Th2 (GATA-3, IL-4) in patients with periodontitis compared to controls (p≤0.002). A positive correlation between IL-17 and RORC2 (p≤0.05) and between IL-4 and GATA-3 (p≤0.001) was found.The results show that expression levels of IL-4, GATA-3, IL-17 and RORC2 increase significantly in periodontal lesions compared with the controls. In periodontal lesions, IL-17 levels are significantly greater than IL-4, which plays a protective role against alveolar bone loss. |
| first_indexed | 2024-12-23T10:17:00Z |
| format | Article |
| id | doaj.art-d20ef9ba103b4af0b3a76967fabbf3d9 |
| institution | Directory Open Access Journal |
| issn | 2676-296X |
| language | English |
| last_indexed | 2024-12-23T10:17:00Z |
| publishDate | 2013-01-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Frontiers in Dentistry |
| spelling | doaj.art-d20ef9ba103b4af0b3a76967fabbf3d92022-12-21T17:50:47ZengTehran University of Medical SciencesFrontiers in Dentistry2676-296X2013-01-01101Expression levels of th-2 and th-17 characteristic genes in healthy tissue versus periodontitis.Parichehr Behfarnia0Reza Birang1Seyed Sajad Pishva2Mazdak Ghanjalikhani Hakemi3Mitra Mohammadi Khorasani4Assistant Professor, Periodontics Department, Dental School, Isfahan University of Medical Sciences, Isfahan, Iran. The T helper 17 (Th17) cells have been suggested to have osteoclast activating effects while T helper 2 (Th2) cells are considered to have an osteoprotective role in periodontitis. This study was to compare the markers of Th17 cells (RORC2 and IL-17 genes) with that of Th2 cells (IL-4 and GATA-3 genes) between healthy and periodontitis tissues.The samples were obtained from patients with periodontitis and healthy tissues. The mRNA expression levels of IL-17, RORC2, IL-4 and GATA-3 were measured in both groups using quantitative RT-PCR. The results were compared using SPSS 11.0. Correlation coefficient was analyzed by Spearman's rho test. Mann-Whitney was used to measure the difference between IL-17 and IL-4 as well as RORC2 and GATA-3.In periodontal lesions, the expression levels of all markers were significantly higher than the healthy tissue (p≤ 0.001). The results showed a significant increase in the number of markers of Th17 (RORC2 and IL-17 genes) compared to markers of Th2 (GATA-3, IL-4) in patients with periodontitis compared to controls (p≤0.002). A positive correlation between IL-17 and RORC2 (p≤0.05) and between IL-4 and GATA-3 (p≤0.001) was found.The results show that expression levels of IL-4, GATA-3, IL-17 and RORC2 increase significantly in periodontal lesions compared with the controls. In periodontal lesions, IL-17 levels are significantly greater than IL-4, which plays a protective role against alveolar bone loss.https://jdt.tums.ac.ir/index.php/jdt/article/view/711GATA3 factorsIL-17F proteinPeriodontal DiseaseTH17 cellTH2 cell |
| spellingShingle | Parichehr Behfarnia Reza Birang Seyed Sajad Pishva Mazdak Ghanjalikhani Hakemi Mitra Mohammadi Khorasani Expression levels of th-2 and th-17 characteristic genes in healthy tissue versus periodontitis. Frontiers in Dentistry GATA3 factors IL-17F protein Periodontal Disease TH17 cell TH2 cell |
| title | Expression levels of th-2 and th-17 characteristic genes in healthy tissue versus periodontitis. |
| title_full | Expression levels of th-2 and th-17 characteristic genes in healthy tissue versus periodontitis. |
| title_fullStr | Expression levels of th-2 and th-17 characteristic genes in healthy tissue versus periodontitis. |
| title_full_unstemmed | Expression levels of th-2 and th-17 characteristic genes in healthy tissue versus periodontitis. |
| title_short | Expression levels of th-2 and th-17 characteristic genes in healthy tissue versus periodontitis. |
| title_sort | expression levels of th 2 and th 17 characteristic genes in healthy tissue versus periodontitis |
| topic | GATA3 factors IL-17F protein Periodontal Disease TH17 cell TH2 cell |
| url | https://jdt.tums.ac.ir/index.php/jdt/article/view/711 |
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