Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves)
The selection of molecular markers for population studies is an important tool for biodiversity conservation. The family Psittacidae contains many endangered and vulnerable species and we tested three kinds of molecular markers for their potential use in population studies of five psitacid species:...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2006-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200007 |
| _version_ | 1819260678606684160 |
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| author | Patrícia J. Faria Cristina Y. Miyaki |
| author_facet | Patrícia J. Faria Cristina Y. Miyaki |
| author_sort | Patrícia J. Faria |
| collection | DOAJ |
| description | The selection of molecular markers for population studies is an important tool for biodiversity conservation. The family Psittacidae contains many endangered and vulnerable species and we tested three kinds of molecular markers for their potential use in population studies of five psitacid species: 43 hyacinth macaws (Anodorhynchus hyacinthinus), 42 blue-and-yellow macaws (Ara ararauna), 23 red-and-green macaws (Ara chloroptera), 19 red-spectacled amazons (Amazona pretrei); and 18 red-tailed amazons (Amazona brasiliensis). We tested 21 clones from a genomic library of golden conure (Guarouba guarouba) minisatellites and 12 pairs of microsatellite primers developed for the domestic chicken (Gallus gallus) and A. hyacinthinus. We also tested seven tetranucleotide repeat primers for their ability to amplify regions between microsatellite loci (inter simple sequence repeats, ISSRs). We were able to select seven markers that were variable in different degrees for three species (A. hyacinthinus, A. chloroptera and A. ararauna). The mini and microsatellites produced more polymorphic patterns than the ISSRs. The genetic variability of the species studied seems to be correlated with their endangered status. |
| first_indexed | 2024-12-23T19:29:44Z |
| format | Article |
| id | doaj.art-d210ad2677d345d5a8a73b80c8c1dfab |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-23T19:29:44Z |
| publishDate | 2006-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-d210ad2677d345d5a8a73b80c8c1dfab2022-12-21T17:33:57ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852006-01-0129223124010.1590/S1415-47572006000200007Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves)Patrícia J. FariaCristina Y. MiyakiThe selection of molecular markers for population studies is an important tool for biodiversity conservation. The family Psittacidae contains many endangered and vulnerable species and we tested three kinds of molecular markers for their potential use in population studies of five psitacid species: 43 hyacinth macaws (Anodorhynchus hyacinthinus), 42 blue-and-yellow macaws (Ara ararauna), 23 red-and-green macaws (Ara chloroptera), 19 red-spectacled amazons (Amazona pretrei); and 18 red-tailed amazons (Amazona brasiliensis). We tested 21 clones from a genomic library of golden conure (Guarouba guarouba) minisatellites and 12 pairs of microsatellite primers developed for the domestic chicken (Gallus gallus) and A. hyacinthinus. We also tested seven tetranucleotide repeat primers for their ability to amplify regions between microsatellite loci (inter simple sequence repeats, ISSRs). We were able to select seven markers that were variable in different degrees for three species (A. hyacinthinus, A. chloroptera and A. ararauna). The mini and microsatellites produced more polymorphic patterns than the ISSRs. The genetic variability of the species studied seems to be correlated with their endangered status.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200007microsatellitesminisatellitesmolecular markersparrotspopulation studies |
| spellingShingle | Patrícia J. Faria Cristina Y. Miyaki Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves) Genetics and Molecular Biology microsatellites minisatellites molecular markers parrots population studies |
| title | Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves) |
| title_full | Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves) |
| title_fullStr | Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves) |
| title_full_unstemmed | Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves) |
| title_short | Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves) |
| title_sort | molecular markers for population genetic analyses in the family psittacidae psittaciformes aves |
| topic | microsatellites minisatellites molecular markers parrots population studies |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200007 |
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