Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in Plants
RNAs play important roles in regulating biological growth and development. Advancements in RNA-imaging techniques are expanding our understanding of their function. Several common RNA-labeling methods in plants have pros and cons. Simultaneously, plants’ spontaneously fluorescent substances interfer...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-03-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/13/5/743 |
| _version_ | 1797264042351394816 |
|---|---|
| author | Zheng-Chao Yang Li-Xiang Zhao Yu-Qi Sang Xin Huang Xuan-Chen Lin Zhi-Ming Yu |
| author_facet | Zheng-Chao Yang Li-Xiang Zhao Yu-Qi Sang Xin Huang Xuan-Chen Lin Zhi-Ming Yu |
| author_sort | Zheng-Chao Yang |
| collection | DOAJ |
| description | RNAs play important roles in regulating biological growth and development. Advancements in RNA-imaging techniques are expanding our understanding of their function. Several common RNA-labeling methods in plants have pros and cons. Simultaneously, plants’ spontaneously fluorescent substances interfere with the effectiveness of RNA bioimaging. New technologies need to be introduced into plant RNA luminescence. Aggregation-induced emission luminogens (AIEgens), due to their luminescent properties, tunable molecular size, high fluorescence intensity, good photostability, and low cell toxicity, have been widely applied in the animal and medical fields. The application of this technology in plants is still at an early stage. The development of AIEgens provides more options for RNA labeling. Click chemistry provides ideas for modifying AIEgens into RNA molecules. The CRISPR/Cas13a-mediated targeting system provides a guarantee of precise RNA modification. The liquid–liquid phase separation in plant cells creates conditions for the enrichment and luminescence of AIEgens. The only thing that needs to be looked for is a specific enzyme that uses AIEgens as a substrate and modifies AIEgens onto target RNA via a click chemical reaction. With the development and progress of artificial intelligence and synthetic biology, it may soon be possible to artificially synthesize or discover such an enzyme. |
| first_indexed | 2024-04-25T00:22:36Z |
| format | Article |
| id | doaj.art-d2130de29b414fddbd68787e0def7cff |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-04-25T00:22:36Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-d2130de29b414fddbd68787e0def7cff2024-03-12T16:53:13ZengMDPI AGPlants2223-77472024-03-0113574310.3390/plants13050743Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in PlantsZheng-Chao Yang0Li-Xiang Zhao1Yu-Qi Sang2Xin Huang3Xuan-Chen Lin4Zhi-Ming Yu5College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaRNAs play important roles in regulating biological growth and development. Advancements in RNA-imaging techniques are expanding our understanding of their function. Several common RNA-labeling methods in plants have pros and cons. Simultaneously, plants’ spontaneously fluorescent substances interfere with the effectiveness of RNA bioimaging. New technologies need to be introduced into plant RNA luminescence. Aggregation-induced emission luminogens (AIEgens), due to their luminescent properties, tunable molecular size, high fluorescence intensity, good photostability, and low cell toxicity, have been widely applied in the animal and medical fields. The application of this technology in plants is still at an early stage. The development of AIEgens provides more options for RNA labeling. Click chemistry provides ideas for modifying AIEgens into RNA molecules. The CRISPR/Cas13a-mediated targeting system provides a guarantee of precise RNA modification. The liquid–liquid phase separation in plant cells creates conditions for the enrichment and luminescence of AIEgens. The only thing that needs to be looked for is a specific enzyme that uses AIEgens as a substrate and modifies AIEgens onto target RNA via a click chemical reaction. With the development and progress of artificial intelligence and synthetic biology, it may soon be possible to artificially synthesize or discover such an enzyme.https://www.mdpi.com/2223-7747/13/5/743aggregation-induced emission (AIE)RNA fluorescence labelingclick chemistryRNA aptamer |
| spellingShingle | Zheng-Chao Yang Li-Xiang Zhao Yu-Qi Sang Xin Huang Xuan-Chen Lin Zhi-Ming Yu Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in Plants Plants aggregation-induced emission (AIE) RNA fluorescence labeling click chemistry RNA aptamer |
| title | Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in Plants |
| title_full | Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in Plants |
| title_fullStr | Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in Plants |
| title_full_unstemmed | Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in Plants |
| title_short | Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in Plants |
| title_sort | aggregation induced emission luminogens a new possibility for efficient visualization of rna in plants |
| topic | aggregation-induced emission (AIE) RNA fluorescence labeling click chemistry RNA aptamer |
| url | https://www.mdpi.com/2223-7747/13/5/743 |
| work_keys_str_mv | AT zhengchaoyang aggregationinducedemissionluminogensanewpossibilityforefficientvisualizationofrnainplants AT lixiangzhao aggregationinducedemissionluminogensanewpossibilityforefficientvisualizationofrnainplants AT yuqisang aggregationinducedemissionluminogensanewpossibilityforefficientvisualizationofrnainplants AT xinhuang aggregationinducedemissionluminogensanewpossibilityforefficientvisualizationofrnainplants AT xuanchenlin aggregationinducedemissionluminogensanewpossibilityforefficientvisualizationofrnainplants AT zhimingyu aggregationinducedemissionluminogensanewpossibilityforefficientvisualizationofrnainplants |