Extraction Methods, Chemical Characterization, and In Vitro Biological Activities of <i>Plinia cauliflora</i> (Mart.) Kausel Peels
<i>Plinia cauliflora</i> (Mart.) Kausel, popularly known as jabuticaba, possesses bioactive compounds such as flavonoids, tannins, and phenolic acids, known for their antioxidant, antibacterial, wound healing, and cardioprotective effects. Therefore, this study aimed to standardize the &...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/16/8/1173 |
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| author | Mariana Moraes Pinc Mariana Dalmagro Elton da Cruz Alves Pereira Guilherme Donadel Renan Tedeski Thomaz Camila da Silva Paula Derksen Macruz Ezilda Jacomassi Arquimedes Gasparotto Junior Jaqueline Hoscheid Emerson Luiz Botelho Lourenço Odair Alberton |
| author_facet | Mariana Moraes Pinc Mariana Dalmagro Elton da Cruz Alves Pereira Guilherme Donadel Renan Tedeski Thomaz Camila da Silva Paula Derksen Macruz Ezilda Jacomassi Arquimedes Gasparotto Junior Jaqueline Hoscheid Emerson Luiz Botelho Lourenço Odair Alberton |
| author_sort | Mariana Moraes Pinc |
| collection | DOAJ |
| description | <i>Plinia cauliflora</i> (Mart.) Kausel, popularly known as jabuticaba, possesses bioactive compounds such as flavonoids, tannins, and phenolic acids, known for their antioxidant, antibacterial, wound healing, and cardioprotective effects. Therefore, this study aimed to standardize the <i>P. cauliflora</i> fruit peel extraction method, maximize phenolic constituents, and evaluate their antioxidative and antimicrobial effects. Various extraction methods, including vortex extraction with and without precipitation at 25, 40, and 80 °C, and infusion extraction with and without precipitation, were performed using a completely randomized design. Extraction without precipitation (E − P) showed the highest yield (57.9%). However, the precipitated extraction (E + P) method displayed a yield of 45.9%, higher levels of phenolic derivatives, and enhanced antioxidant capacity. Major compounds, such as D-psicose, D-glucose, and citric acid, were identified through gas chromatography–mass spectrometry (GC-MS) analysis. Ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) analysis identified citric acid, hexose, flavonoids, tannins, and quercetin as the major compounds in the extracts. Furthermore, the extracts exhibited inhibitory effects against <i>Bacillus subtilis</i>, <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i>, and <i>Escherichia coli</i> bacteria. In conclusion, the E + P method efficiently obtained extracts with high content of bioactive compounds showing antioxidant and antimicrobial capacities with potential application as a dietary supplement. |
| first_indexed | 2024-03-10T23:39:58Z |
| format | Article |
| id | doaj.art-d219061772814e809ab10e82364228fc |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T23:39:58Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-d219061772814e809ab10e82364228fc2023-11-19T02:35:09ZengMDPI AGPharmaceuticals1424-82472023-08-01168117310.3390/ph16081173Extraction Methods, Chemical Characterization, and In Vitro Biological Activities of <i>Plinia cauliflora</i> (Mart.) Kausel PeelsMariana Moraes Pinc0Mariana Dalmagro1Elton da Cruz Alves Pereira2Guilherme Donadel3Renan Tedeski Thomaz4Camila da Silva5Paula Derksen Macruz6Ezilda Jacomassi7Arquimedes Gasparotto Junior8Jaqueline Hoscheid9Emerson Luiz Botelho Lourenço10Odair Alberton11Laboratory of Preclinical Research of Natural Products, Paranaense University, Umuarama 87502-210, Paraná, BrazilLaboratory of Preclinical Research of Natural Products, Paranaense University, Umuarama 87502-210, Paraná, BrazilLaboratory of Preclinical Research of Natural Products, Paranaense University, Umuarama 87502-210, Paraná, BrazilLaboratory of Preclinical Research of Natural Products, Paranaense University, Umuarama 87502-210, Paraná, BrazilLaboratory of Preclinical Research of Natural Products, Paranaense University, Umuarama 87502-210, Paraná, BrazilDepartment of Technology, State University of Maringá, Umuarama 87506-370, Paraná, BrazilDepartment of Chemical Engineering, State University of Maringá, Maringá 87020-900, Paraná, BrazilLaboratory of Preclinical Research of Natural Products, Paranaense University, Umuarama 87502-210, Paraná, BrazilLaboratory of Cardiovascular Pharmacology (LaFaC), Faculty of Health Sciences, Federal University of Grande Dourados, Dourados 79804-970, Mato Grosso do Sul, BrazilLaboratory of Preclinical Research of Natural Products, Paranaense University, Umuarama 87502-210, Paraná, BrazilLaboratory of Preclinical Research of Natural Products, Paranaense University, Umuarama 87502-210, Paraná, BrazilLaboratory of Preclinical Research of Natural Products, Paranaense University, Umuarama 87502-210, Paraná, Brazil<i>Plinia cauliflora</i> (Mart.) Kausel, popularly known as jabuticaba, possesses bioactive compounds such as flavonoids, tannins, and phenolic acids, known for their antioxidant, antibacterial, wound healing, and cardioprotective effects. Therefore, this study aimed to standardize the <i>P. cauliflora</i> fruit peel extraction method, maximize phenolic constituents, and evaluate their antioxidative and antimicrobial effects. Various extraction methods, including vortex extraction with and without precipitation at 25, 40, and 80 °C, and infusion extraction with and without precipitation, were performed using a completely randomized design. Extraction without precipitation (E − P) showed the highest yield (57.9%). However, the precipitated extraction (E + P) method displayed a yield of 45.9%, higher levels of phenolic derivatives, and enhanced antioxidant capacity. Major compounds, such as D-psicose, D-glucose, and citric acid, were identified through gas chromatography–mass spectrometry (GC-MS) analysis. Ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) analysis identified citric acid, hexose, flavonoids, tannins, and quercetin as the major compounds in the extracts. Furthermore, the extracts exhibited inhibitory effects against <i>Bacillus subtilis</i>, <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i>, and <i>Escherichia coli</i> bacteria. In conclusion, the E + P method efficiently obtained extracts with high content of bioactive compounds showing antioxidant and antimicrobial capacities with potential application as a dietary supplement.https://www.mdpi.com/1424-8247/16/8/1173infusionMyrtaceaevortex extractionstandardizationantioxidant |
| spellingShingle | Mariana Moraes Pinc Mariana Dalmagro Elton da Cruz Alves Pereira Guilherme Donadel Renan Tedeski Thomaz Camila da Silva Paula Derksen Macruz Ezilda Jacomassi Arquimedes Gasparotto Junior Jaqueline Hoscheid Emerson Luiz Botelho Lourenço Odair Alberton Extraction Methods, Chemical Characterization, and In Vitro Biological Activities of <i>Plinia cauliflora</i> (Mart.) Kausel Peels Pharmaceuticals infusion Myrtaceae vortex extraction standardization antioxidant |
| title | Extraction Methods, Chemical Characterization, and In Vitro Biological Activities of <i>Plinia cauliflora</i> (Mart.) Kausel Peels |
| title_full | Extraction Methods, Chemical Characterization, and In Vitro Biological Activities of <i>Plinia cauliflora</i> (Mart.) Kausel Peels |
| title_fullStr | Extraction Methods, Chemical Characterization, and In Vitro Biological Activities of <i>Plinia cauliflora</i> (Mart.) Kausel Peels |
| title_full_unstemmed | Extraction Methods, Chemical Characterization, and In Vitro Biological Activities of <i>Plinia cauliflora</i> (Mart.) Kausel Peels |
| title_short | Extraction Methods, Chemical Characterization, and In Vitro Biological Activities of <i>Plinia cauliflora</i> (Mart.) Kausel Peels |
| title_sort | extraction methods chemical characterization and in vitro biological activities of i plinia cauliflora i mart kausel peels |
| topic | infusion Myrtaceae vortex extraction standardization antioxidant |
| url | https://www.mdpi.com/1424-8247/16/8/1173 |
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