HOG1 Mitogen-Activated Protein Kinase Pathway–Related Autophagy Induced by H<sub>2</sub>O<sub>2</sub> in <i>Lentinula edodes</i> Mycelia
Mycelial ageing is associated with ROS and autophagy in <i>Lentinula edodes</i>. However, the underlying cellular and molecular mechanisms between ROS and autophagy remain obscure. This study induced autophagy in <i>L. edodes</i> mycelia through exogenous H<sub>2</su...
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MDPI AG
2023-03-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/4/413 |
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| author | Dong Yan Yangyang Fan Shuang Song Yuan Guo Yu Liu Xiaoling Xu Fang Liu Qi Gao Shouxian Wang |
| author_facet | Dong Yan Yangyang Fan Shuang Song Yuan Guo Yu Liu Xiaoling Xu Fang Liu Qi Gao Shouxian Wang |
| author_sort | Dong Yan |
| collection | DOAJ |
| description | Mycelial ageing is associated with ROS and autophagy in <i>Lentinula edodes</i>. However, the underlying cellular and molecular mechanisms between ROS and autophagy remain obscure. This study induced autophagy in <i>L. edodes</i> mycelia through exogenous H<sub>2</sub>O<sub>2</sub> treatment. Results showed that 100 μM H<sub>2</sub>O<sub>2</sub> treatment for 24 h significantly inhibited mycelial growth. H<sub>2</sub>O<sub>2</sub> caused the depolarisation of MMP and accumulation of TUNEL-positive nuclei, which was similar to the ageing phenotype of <i>L. edodes</i> mycelia. Transcriptome analysis showed that differentially expressed genes were enriched in the mitophagic, autophagic, and MAPK pathways. <i>LeAtg8</i> and <i>LeHog1</i> were selected as hub genes. RNA and protein levels of LeATG8 increased in the H<sub>2</sub>O<sub>2</sub>-treated mycelia. Using fluorescent labelling, we observed for the first time the classic ring structure of autophagosomes in a mushroom, while 3D imaging suggested that these autophagosomes surrounded the nuclei to degrade them at specific growth stages. Phospho-LeHOG1 protein can translocate from the cytoplasm to the nucleus to regulate mycelial cells, resisting ROS-induced oxidative stress. Furthermore, LeATG8 expression was suppressed when LeHOG1 phosphorylation was inhibited. These results suggest that the LeATG8-dependent autophagy in <i>L. edodes</i> mycelial is closely associated with the activity or even phosphorylation of LeHOG1. |
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| language | English |
| last_indexed | 2024-03-11T04:52:24Z |
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| spelling | doaj.art-d219c95816024741b168e399a0fde7f12023-11-17T19:57:48ZengMDPI AGJournal of Fungi2309-608X2023-03-019441310.3390/jof9040413HOG1 Mitogen-Activated Protein Kinase Pathway–Related Autophagy Induced by H<sub>2</sub>O<sub>2</sub> in <i>Lentinula edodes</i> MyceliaDong Yan0Yangyang Fan1Shuang Song2Yuan Guo3Yu Liu4Xiaoling Xu5Fang Liu6Qi Gao7Shouxian Wang8Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, 9 Shuguang Garden Zhonglu, Haidian District, Beijing 100097, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, 9 Shuguang Garden Zhonglu, Haidian District, Beijing 100097, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, 9 Shuguang Garden Zhonglu, Haidian District, Beijing 100097, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, 9 Shuguang Garden Zhonglu, Haidian District, Beijing 100097, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, 9 Shuguang Garden Zhonglu, Haidian District, Beijing 100097, ChinaCollege of Agriculture and Food Engineering, Baise University, 21 Zhongshan Second Street, Youjiang District, Baise 533000, ChinaCollege of Agriculture and Food Engineering, Baise University, 21 Zhongshan Second Street, Youjiang District, Baise 533000, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, 9 Shuguang Garden Zhonglu, Haidian District, Beijing 100097, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, 9 Shuguang Garden Zhonglu, Haidian District, Beijing 100097, ChinaMycelial ageing is associated with ROS and autophagy in <i>Lentinula edodes</i>. However, the underlying cellular and molecular mechanisms between ROS and autophagy remain obscure. This study induced autophagy in <i>L. edodes</i> mycelia through exogenous H<sub>2</sub>O<sub>2</sub> treatment. Results showed that 100 μM H<sub>2</sub>O<sub>2</sub> treatment for 24 h significantly inhibited mycelial growth. H<sub>2</sub>O<sub>2</sub> caused the depolarisation of MMP and accumulation of TUNEL-positive nuclei, which was similar to the ageing phenotype of <i>L. edodes</i> mycelia. Transcriptome analysis showed that differentially expressed genes were enriched in the mitophagic, autophagic, and MAPK pathways. <i>LeAtg8</i> and <i>LeHog1</i> were selected as hub genes. RNA and protein levels of LeATG8 increased in the H<sub>2</sub>O<sub>2</sub>-treated mycelia. Using fluorescent labelling, we observed for the first time the classic ring structure of autophagosomes in a mushroom, while 3D imaging suggested that these autophagosomes surrounded the nuclei to degrade them at specific growth stages. Phospho-LeHOG1 protein can translocate from the cytoplasm to the nucleus to regulate mycelial cells, resisting ROS-induced oxidative stress. Furthermore, LeATG8 expression was suppressed when LeHOG1 phosphorylation was inhibited. These results suggest that the LeATG8-dependent autophagy in <i>L. edodes</i> mycelial is closely associated with the activity or even phosphorylation of LeHOG1.https://www.mdpi.com/2309-608X/9/4/413<i>Lentinula edodes</i>H<sub>2</sub>O<sub>2</sub>autophagyLeATG8LeHOG1 |
| spellingShingle | Dong Yan Yangyang Fan Shuang Song Yuan Guo Yu Liu Xiaoling Xu Fang Liu Qi Gao Shouxian Wang HOG1 Mitogen-Activated Protein Kinase Pathway–Related Autophagy Induced by H<sub>2</sub>O<sub>2</sub> in <i>Lentinula edodes</i> Mycelia Journal of Fungi <i>Lentinula edodes</i> H<sub>2</sub>O<sub>2</sub> autophagy LeATG8 LeHOG1 |
| title | HOG1 Mitogen-Activated Protein Kinase Pathway–Related Autophagy Induced by H<sub>2</sub>O<sub>2</sub> in <i>Lentinula edodes</i> Mycelia |
| title_full | HOG1 Mitogen-Activated Protein Kinase Pathway–Related Autophagy Induced by H<sub>2</sub>O<sub>2</sub> in <i>Lentinula edodes</i> Mycelia |
| title_fullStr | HOG1 Mitogen-Activated Protein Kinase Pathway–Related Autophagy Induced by H<sub>2</sub>O<sub>2</sub> in <i>Lentinula edodes</i> Mycelia |
| title_full_unstemmed | HOG1 Mitogen-Activated Protein Kinase Pathway–Related Autophagy Induced by H<sub>2</sub>O<sub>2</sub> in <i>Lentinula edodes</i> Mycelia |
| title_short | HOG1 Mitogen-Activated Protein Kinase Pathway–Related Autophagy Induced by H<sub>2</sub>O<sub>2</sub> in <i>Lentinula edodes</i> Mycelia |
| title_sort | hog1 mitogen activated protein kinase pathway related autophagy induced by h sub 2 sub o sub 2 sub in i lentinula edodes i mycelia |
| topic | <i>Lentinula edodes</i> H<sub>2</sub>O<sub>2</sub> autophagy LeATG8 LeHOG1 |
| url | https://www.mdpi.com/2309-608X/9/4/413 |
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