Small Intestinal Tuft Cell Activity Associates With Energy Metabolism in Diet-Induced Obesity
Little is known about the involvement of type 2 immune response-promoting intestinal tuft cells in metabolic regulation. We here examined the temporal changes in small intestinal tuft cell number and activity in response to high-fat diet-induced obesity in mice and investigated the relation to whole...
| Asıl Yazarlar: | , , , , , , , , , , |
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| Materyal Türü: | Makale |
| Dil: | English |
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Frontiers Media S.A.
2021-05-01
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| Seri Bilgileri: | Frontiers in Immunology |
| Konular: | |
| Online Erişim: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.629391/full |
| _version_ | 1831629141223407616 |
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| author | Pankaj Arora Daniel Andersen Janne Marie Moll Niels Banhos Danneskiold-Samsøe Liqin Xu Liqin Xu Biaofeng Zhou Georgios Kladis Philipp Rausch Christopher T. Workman Karsten Kristiansen Karsten Kristiansen Susanne Brix |
| author_facet | Pankaj Arora Daniel Andersen Janne Marie Moll Niels Banhos Danneskiold-Samsøe Liqin Xu Liqin Xu Biaofeng Zhou Georgios Kladis Philipp Rausch Christopher T. Workman Karsten Kristiansen Karsten Kristiansen Susanne Brix |
| author_sort | Pankaj Arora |
| collection | DOAJ |
| description | Little is known about the involvement of type 2 immune response-promoting intestinal tuft cells in metabolic regulation. We here examined the temporal changes in small intestinal tuft cell number and activity in response to high-fat diet-induced obesity in mice and investigated the relation to whole-body energy metabolism and the immune phenotype of the small intestine and epididymal white adipose tissue. Intake of high fat diet resulted in a reduction in overall numbers of small intestinal epithelial and tuft cells and reduced expression of the intestinal type 2 tuft cell markers Il25 and Tslp. Amongst >1,700 diet-regulated transcripts in tuft cells, we observed an early association between body mass expansion and increased expression of the gene encoding the serine protease inhibitor neuroserpin. By contrast, tuft cell expression of genes encoding gamma aminobutyric acid (GABA)-receptors was coupled to Tslp and Il25 and reduced body mass gain. Combined, our results point to a possible role for small intestinal tuft cells in energy metabolism via coupled regulation of tuft cell type 2 markers and GABA signaling receptors, while being independent of type 2 immune cell involvement. These results pave the way for further studies into interventions that elicit anti-obesogenic circuits via small intestinal tuft cells. |
| first_indexed | 2024-12-19T01:53:38Z |
| format | Article |
| id | doaj.art-d21a5e81f97c493a847fdd917e6bcb27 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-19T01:53:38Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-d21a5e81f97c493a847fdd917e6bcb272022-12-21T20:41:20ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-05-011210.3389/fimmu.2021.629391629391Small Intestinal Tuft Cell Activity Associates With Energy Metabolism in Diet-Induced ObesityPankaj Arora0Daniel Andersen1Janne Marie Moll2Niels Banhos Danneskiold-Samsøe3Liqin Xu4Liqin Xu5Biaofeng Zhou6Georgios Kladis7Philipp Rausch8Christopher T. Workman9Karsten Kristiansen10Karsten Kristiansen11Susanne Brix12Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, DenmarkDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, DenmarkDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, DenmarkLaboratory of Genomics and Molecular Biomedicine, Department of Biology, University of Copenhagen, Copenhagen, DenmarkDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, DenmarkBGI-Shenzhen, Shenzhen, ChinaBGI-Shenzhen, Shenzhen, ChinaDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, DenmarkLaboratory of Genomics and Molecular Biomedicine, Department of Biology, University of Copenhagen, Copenhagen, DenmarkDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, DenmarkLaboratory of Genomics and Molecular Biomedicine, Department of Biology, University of Copenhagen, Copenhagen, DenmarkBGI-Shenzhen, Shenzhen, ChinaDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, DenmarkLittle is known about the involvement of type 2 immune response-promoting intestinal tuft cells in metabolic regulation. We here examined the temporal changes in small intestinal tuft cell number and activity in response to high-fat diet-induced obesity in mice and investigated the relation to whole-body energy metabolism and the immune phenotype of the small intestine and epididymal white adipose tissue. Intake of high fat diet resulted in a reduction in overall numbers of small intestinal epithelial and tuft cells and reduced expression of the intestinal type 2 tuft cell markers Il25 and Tslp. Amongst >1,700 diet-regulated transcripts in tuft cells, we observed an early association between body mass expansion and increased expression of the gene encoding the serine protease inhibitor neuroserpin. By contrast, tuft cell expression of genes encoding gamma aminobutyric acid (GABA)-receptors was coupled to Tslp and Il25 and reduced body mass gain. Combined, our results point to a possible role for small intestinal tuft cells in energy metabolism via coupled regulation of tuft cell type 2 markers and GABA signaling receptors, while being independent of type 2 immune cell involvement. These results pave the way for further studies into interventions that elicit anti-obesogenic circuits via small intestinal tuft cells.https://www.frontiersin.org/articles/10.3389/fimmu.2021.629391/fulltuft cellshigh fat dietgut-brain axisGABAneuroserpinmetabolism |
| spellingShingle | Pankaj Arora Daniel Andersen Janne Marie Moll Niels Banhos Danneskiold-Samsøe Liqin Xu Liqin Xu Biaofeng Zhou Georgios Kladis Philipp Rausch Christopher T. Workman Karsten Kristiansen Karsten Kristiansen Susanne Brix Small Intestinal Tuft Cell Activity Associates With Energy Metabolism in Diet-Induced Obesity Frontiers in Immunology tuft cells high fat diet gut-brain axis GABA neuroserpin metabolism |
| title | Small Intestinal Tuft Cell Activity Associates With Energy Metabolism in Diet-Induced Obesity |
| title_full | Small Intestinal Tuft Cell Activity Associates With Energy Metabolism in Diet-Induced Obesity |
| title_fullStr | Small Intestinal Tuft Cell Activity Associates With Energy Metabolism in Diet-Induced Obesity |
| title_full_unstemmed | Small Intestinal Tuft Cell Activity Associates With Energy Metabolism in Diet-Induced Obesity |
| title_short | Small Intestinal Tuft Cell Activity Associates With Energy Metabolism in Diet-Induced Obesity |
| title_sort | small intestinal tuft cell activity associates with energy metabolism in diet induced obesity |
| topic | tuft cells high fat diet gut-brain axis GABA neuroserpin metabolism |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.629391/full |
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