The role of receptor-mediated T-cells activation disorders in pulmonary tuberculosis
Aim. To analyze the peculiarities and mechanisms of receptor-mediated T-lymphocytes disorders in different clinical forms of pulmonary tuberculosis.Materials and мethods. The study involved 116 patients with first diagnosed infiltrative and disseminated drug-sensitive and drug-resistant pulmonary tu...
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| Format: | Article |
| Language: | English |
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Siberian State Medical University (Tomsk)
2017-07-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/881 |
| _version_ | 1827991547652603904 |
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| author | Irina E. Esimova Olga I. Urazova Vyacheslav V. Novitsky |
| author_facet | Irina E. Esimova Olga I. Urazova Vyacheslav V. Novitsky |
| author_sort | Irina E. Esimova |
| collection | DOAJ |
| description | Aim. To analyze the peculiarities and mechanisms of receptor-mediated T-lymphocytes disorders in different clinical forms of pulmonary tuberculosis.Materials and мethods. The study involved 116 patients with first diagnosed infiltrative and disseminated drug-sensitive and drug-resistant pulmonary tuberculosis. The key stages in receptor-mediated activation of T-lymphocytes, isolated from blood, after their CD3/CD28-induction in vitro with addition of intracellular transport blocker were analyzed. Their immunotyping was carried out with the method of two- and threecolor flow cytofluorometry. The obtained results were statistically analyzed.Results. The breach of extracellular and intracellular stages of T-lymphocytes activation, shown by reduction in total number of CD3- and CD28-positive cells, and CD3+CD28+IL2+, CD3+CD28+IL2–, CD3+NF-kB+, CD3+NFAT2+ lymphocytes, and increase in number of CD3+CTLA4+ cells, was identified with most of their manifestations in disseminated drug-resistant pulmonary tuberculosis. It was shown that the content of CD3+AP-1+ lymphocytes is variable in drug-resistant pulmonary tuberculosis: it increases in the infiltrative form and decreases in the disseminated form.Conclusion. The results showed different mechanisms leading to a deficiency of IL-2-positive lymphocytes and T-lymphocytopenia: from “functional reserve” exhaustion of T-cells in drug-sensitive pulmonary tuberculosis to immunosuppression under the influence of suppressive cytokines (in case of the infiltrative form) and inhibitory protein CTLA4 (in case of the disseminated form) in drug-resistant pulmonary tuberculosis. |
| first_indexed | 2024-04-10T01:06:52Z |
| format | Article |
| id | doaj.art-d21acb04c3114e80864791ab48d864b6 |
| institution | Directory Open Access Journal |
| issn | 1682-0363 1819-3684 |
| language | English |
| last_indexed | 2024-04-10T01:06:52Z |
| publishDate | 2017-07-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj.art-d21acb04c3114e80864791ab48d864b62023-03-13T09:58:23ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842017-07-0116211412410.20538/1682-0363-2017-2-114-124670The role of receptor-mediated T-cells activation disorders in pulmonary tuberculosisIrina E. Esimova0Olga I. Urazova1Vyacheslav V. Novitsky2Сибирский государственный медицинский университет (СибГМУ)Сибирский государственный медицинский университет (СибГМУ)Сибирский государственный медицинский университет (СибГМУ)Aim. To analyze the peculiarities and mechanisms of receptor-mediated T-lymphocytes disorders in different clinical forms of pulmonary tuberculosis.Materials and мethods. The study involved 116 patients with first diagnosed infiltrative and disseminated drug-sensitive and drug-resistant pulmonary tuberculosis. The key stages in receptor-mediated activation of T-lymphocytes, isolated from blood, after their CD3/CD28-induction in vitro with addition of intracellular transport blocker were analyzed. Their immunotyping was carried out with the method of two- and threecolor flow cytofluorometry. The obtained results were statistically analyzed.Results. The breach of extracellular and intracellular stages of T-lymphocytes activation, shown by reduction in total number of CD3- and CD28-positive cells, and CD3+CD28+IL2+, CD3+CD28+IL2–, CD3+NF-kB+, CD3+NFAT2+ lymphocytes, and increase in number of CD3+CTLA4+ cells, was identified with most of their manifestations in disseminated drug-resistant pulmonary tuberculosis. It was shown that the content of CD3+AP-1+ lymphocytes is variable in drug-resistant pulmonary tuberculosis: it increases in the infiltrative form and decreases in the disseminated form.Conclusion. The results showed different mechanisms leading to a deficiency of IL-2-positive lymphocytes and T-lymphocytopenia: from “functional reserve” exhaustion of T-cells in drug-sensitive pulmonary tuberculosis to immunosuppression under the influence of suppressive cytokines (in case of the infiltrative form) and inhibitory protein CTLA4 (in case of the disseminated form) in drug-resistant pulmonary tuberculosis.https://bulletin.ssmu.ru/jour/article/view/881туберкулезиммунитетт-лимфоцитыт-клеточный рецепториммуносупрессия |
| spellingShingle | Irina E. Esimova Olga I. Urazova Vyacheslav V. Novitsky The role of receptor-mediated T-cells activation disorders in pulmonary tuberculosis Бюллетень сибирской медицины туберкулез иммунитет т-лимфоциты т-клеточный рецептор иммуносупрессия |
| title | The role of receptor-mediated T-cells activation disorders in pulmonary tuberculosis |
| title_full | The role of receptor-mediated T-cells activation disorders in pulmonary tuberculosis |
| title_fullStr | The role of receptor-mediated T-cells activation disorders in pulmonary tuberculosis |
| title_full_unstemmed | The role of receptor-mediated T-cells activation disorders in pulmonary tuberculosis |
| title_short | The role of receptor-mediated T-cells activation disorders in pulmonary tuberculosis |
| title_sort | role of receptor mediated t cells activation disorders in pulmonary tuberculosis |
| topic | туберкулез иммунитет т-лимфоциты т-клеточный рецептор иммуносупрессия |
| url | https://bulletin.ssmu.ru/jour/article/view/881 |
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