Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male rats
Objectives: In previous studies the therapeutic effects of Nigella sativa have been demonstrated on asthmatic animals. In the present study, the preventive effect of single dose of alpha-hederin, its active constituent, has been evaluated on lung inflammation and some inflammatory mediators in lungs...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2016-01-01
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| Series: | Avicenna Journal of Phytomedicine |
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| Online Access: | http://ajp.mums.ac.ir/pdf_4622_eb1e17b7fc41beaaceda6589f909135c.html |
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| author | Maryam Fallahi Rana Keyhanmanesh Amir Mahdi Khamaneh Mohammad Ali Ebrahimi Saadatlou Saeideh Saadat Hadi Ebrahimi |
| author_facet | Maryam Fallahi Rana Keyhanmanesh Amir Mahdi Khamaneh Mohammad Ali Ebrahimi Saadatlou Saeideh Saadat Hadi Ebrahimi |
| author_sort | Maryam Fallahi |
| collection | DOAJ |
| description | Objectives: In previous studies the therapeutic effects of Nigella sativa have been demonstrated on asthmatic animals. In the present study, the preventive effect of single dose of alpha-hederin, its active constituent, has been evaluated on lung inflammation and some inflammatory mediators in lungs of ovalbumin sensitized rat in order to elicit its mechanism. Materials and methods: Forty rats were randomly grouped in 4 groups; control (C), sensitized (S), sensitized pretreated groups with thymoquinone (3 mg/kg i.p., S+TQ) and alpha-hederin (0.02 mg/kg i.p., S+AH). Levels of IL-13 mRNA and miRNA-126 in lung tissue and its pathological changes in each group were assessed. Results: Elevated levels of miRNA-126, IL-13 mRNA and pathological changes were observed in the sensitized group compared to the control group (pConclusion: The results suggested that alpha-hederin had preventive effect on sensitized rats like thymoquinone. It may intervene in miRNA-126 expression, which consequently could interfere with IL-13 secretion pathway leading to a reduction in inflammatory responses. |
| first_indexed | 2024-12-20T03:55:22Z |
| format | Article |
| id | doaj.art-d21bcfaafbdf4088bfae9784ce530f30 |
| institution | Directory Open Access Journal |
| issn | 2228-7930 2228-7949 |
| language | English |
| last_indexed | 2024-12-20T03:55:22Z |
| publishDate | 2016-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Phytomedicine |
| spelling | doaj.art-d21bcfaafbdf4088bfae9784ce530f302022-12-21T19:54:21ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492016-01-016177854622Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male ratsMaryam Fallahi0Rana Keyhanmanesh1Amir Mahdi Khamaneh2Mohammad Ali Ebrahimi Saadatlou3Saeideh Saadat4Hadi Ebrahimi5Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IranMedical Education Research Center, Tabriz University of Medical Sciences, Tabriz, IranDepartment of molecular medicine, School of advanced medical sciences, Tabriz University of Medical Sciences, Tabriz, IranDepartment of basic sciences, College of veterinary medicine, Tabriz branch, Islamic Azad University, Tabriz, IranDepartment of Physiology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, IranTuberculosis and lung diseases research center, Tabriz University of Medical Sciences, Tabriz, IranObjectives: In previous studies the therapeutic effects of Nigella sativa have been demonstrated on asthmatic animals. In the present study, the preventive effect of single dose of alpha-hederin, its active constituent, has been evaluated on lung inflammation and some inflammatory mediators in lungs of ovalbumin sensitized rat in order to elicit its mechanism. Materials and methods: Forty rats were randomly grouped in 4 groups; control (C), sensitized (S), sensitized pretreated groups with thymoquinone (3 mg/kg i.p., S+TQ) and alpha-hederin (0.02 mg/kg i.p., S+AH). Levels of IL-13 mRNA and miRNA-126 in lung tissue and its pathological changes in each group were assessed. Results: Elevated levels of miRNA-126, IL-13 mRNA and pathological changes were observed in the sensitized group compared to the control group (pConclusion: The results suggested that alpha-hederin had preventive effect on sensitized rats like thymoquinone. It may intervene in miRNA-126 expression, which consequently could interfere with IL-13 secretion pathway leading to a reduction in inflammatory responses.http://ajp.mums.ac.ir/pdf_4622_eb1e17b7fc41beaaceda6589f909135c.htmlAlpha-hederinMiRNA-126IL-13 mRNAOvalbuminThymoquinoneAsthma |
| spellingShingle | Maryam Fallahi Rana Keyhanmanesh Amir Mahdi Khamaneh Mohammad Ali Ebrahimi Saadatlou Saeideh Saadat Hadi Ebrahimi Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male rats Avicenna Journal of Phytomedicine Alpha-hederin MiRNA-126 IL-13 mRNA Ovalbumin Thymoquinone Asthma |
| title | Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male rats |
| title_full | Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male rats |
| title_fullStr | Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male rats |
| title_full_unstemmed | Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male rats |
| title_short | Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male rats |
| title_sort | effect of alpha hederin the active constituent of nigella sativa on mirna 126 il 13 mrna levels and inflammation of lungs in ovalbumin sensitized male rats |
| topic | Alpha-hederin MiRNA-126 IL-13 mRNA Ovalbumin Thymoquinone Asthma |
| url | http://ajp.mums.ac.ir/pdf_4622_eb1e17b7fc41beaaceda6589f909135c.html |
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