An Analysis of Differentially Expressed Coding and Long Non-Coding RNAs in Multiple Models of Skeletal Muscle Atrophy
The loss of skeletal muscle mass (muscle atrophy or wasting) caused by aging, diseases, and injury decreases quality of life, survival rates, and healthy life expectancy in humans. Although long non-coding RNAs (lncRNAs) have been implicated in skeletal muscle formation and differentiation, their pr...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/5/2558 |
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| author | Keisuke Hitachi Masashi Nakatani Yuri Kiyofuji Hidehito Inagaki Hiroki Kurahashi Kunihiro Tsuchida |
| author_facet | Keisuke Hitachi Masashi Nakatani Yuri Kiyofuji Hidehito Inagaki Hiroki Kurahashi Kunihiro Tsuchida |
| author_sort | Keisuke Hitachi |
| collection | DOAJ |
| description | The loss of skeletal muscle mass (muscle atrophy or wasting) caused by aging, diseases, and injury decreases quality of life, survival rates, and healthy life expectancy in humans. Although long non-coding RNAs (lncRNAs) have been implicated in skeletal muscle formation and differentiation, their precise roles in muscle atrophy remain unclear. In this study, we used RNA-sequencing (RNA-Seq) to examine changes in the expression of lncRNAs in four muscle atrophy conditions (denervation, casting, fasting, and cancer cachexia) in mice. We successfully identified 33 annotated lncRNAs and 18 novel lncRNAs with common expression changes in all four muscle atrophy conditions. Furthermore, an analysis of lncRNA–mRNA correlations revealed that several lncRNAs affected small molecule biosynthetic processes during muscle atrophy. These results provide novel insights into the lncRNA-mediated regulatory mechanism underlying muscle atrophy and may be useful for the identification of promising therapeutic targets. |
| first_indexed | 2024-03-09T05:38:21Z |
| format | Article |
| id | doaj.art-d21e6fb71e2e49808af0759b08ba7528 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:38:21Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d21e6fb71e2e49808af0759b08ba75282023-12-03T12:27:26ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01225255810.3390/ijms22052558An Analysis of Differentially Expressed Coding and Long Non-Coding RNAs in Multiple Models of Skeletal Muscle AtrophyKeisuke Hitachi0Masashi Nakatani1Yuri Kiyofuji2Hidehito Inagaki3Hiroki Kurahashi4Kunihiro Tsuchida5Division for Therapies against Intractable Diseases, Institute for Comprehensive Medical Science (ICMS), Fujita Health University, Toyoake 470-1192, JapanDivision for Therapies against Intractable Diseases, Institute for Comprehensive Medical Science (ICMS), Fujita Health University, Toyoake 470-1192, JapanDivision for Therapies against Intractable Diseases, Institute for Comprehensive Medical Science (ICMS), Fujita Health University, Toyoake 470-1192, JapanGenome and Transcriptome Analysis Center, Fujita Health University, Toyoake 470-1192, JapanGenome and Transcriptome Analysis Center, Fujita Health University, Toyoake 470-1192, JapanDivision for Therapies against Intractable Diseases, Institute for Comprehensive Medical Science (ICMS), Fujita Health University, Toyoake 470-1192, JapanThe loss of skeletal muscle mass (muscle atrophy or wasting) caused by aging, diseases, and injury decreases quality of life, survival rates, and healthy life expectancy in humans. Although long non-coding RNAs (lncRNAs) have been implicated in skeletal muscle formation and differentiation, their precise roles in muscle atrophy remain unclear. In this study, we used RNA-sequencing (RNA-Seq) to examine changes in the expression of lncRNAs in four muscle atrophy conditions (denervation, casting, fasting, and cancer cachexia) in mice. We successfully identified 33 annotated lncRNAs and 18 novel lncRNAs with common expression changes in all four muscle atrophy conditions. Furthermore, an analysis of lncRNA–mRNA correlations revealed that several lncRNAs affected small molecule biosynthetic processes during muscle atrophy. These results provide novel insights into the lncRNA-mediated regulatory mechanism underlying muscle atrophy and may be useful for the identification of promising therapeutic targets.https://www.mdpi.com/1422-0067/22/5/2558long non-coding RNAmRNAskeletal muscle atrophyRNA-sequencing |
| spellingShingle | Keisuke Hitachi Masashi Nakatani Yuri Kiyofuji Hidehito Inagaki Hiroki Kurahashi Kunihiro Tsuchida An Analysis of Differentially Expressed Coding and Long Non-Coding RNAs in Multiple Models of Skeletal Muscle Atrophy International Journal of Molecular Sciences long non-coding RNA mRNA skeletal muscle atrophy RNA-sequencing |
| title | An Analysis of Differentially Expressed Coding and Long Non-Coding RNAs in Multiple Models of Skeletal Muscle Atrophy |
| title_full | An Analysis of Differentially Expressed Coding and Long Non-Coding RNAs in Multiple Models of Skeletal Muscle Atrophy |
| title_fullStr | An Analysis of Differentially Expressed Coding and Long Non-Coding RNAs in Multiple Models of Skeletal Muscle Atrophy |
| title_full_unstemmed | An Analysis of Differentially Expressed Coding and Long Non-Coding RNAs in Multiple Models of Skeletal Muscle Atrophy |
| title_short | An Analysis of Differentially Expressed Coding and Long Non-Coding RNAs in Multiple Models of Skeletal Muscle Atrophy |
| title_sort | analysis of differentially expressed coding and long non coding rnas in multiple models of skeletal muscle atrophy |
| topic | long non-coding RNA mRNA skeletal muscle atrophy RNA-sequencing |
| url | https://www.mdpi.com/1422-0067/22/5/2558 |
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