Potential of the quorum‐quenching and plant‐growth promoting halotolerant Bacillus toyonensis AA1EC1 as biocontrol agent
Abstract The use of fertilizers and pesticides to control plant diseases is widespread in intensive farming causing adverse effects together with the development of antimicrobial resistance pathogens. As the virulence of many Gram‐negative phytopathogens is controlled by N‐acyl‐homoserine lactones (...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-03-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.14420 |
| _version_ | 1797238497449345024 |
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| author | Amalia Roca Mónica Cabeo Carlos Enguidanos Fernando Martínez‐Checa Inmaculada Sampedro Inmaculada Llamas |
| author_facet | Amalia Roca Mónica Cabeo Carlos Enguidanos Fernando Martínez‐Checa Inmaculada Sampedro Inmaculada Llamas |
| author_sort | Amalia Roca |
| collection | DOAJ |
| description | Abstract The use of fertilizers and pesticides to control plant diseases is widespread in intensive farming causing adverse effects together with the development of antimicrobial resistance pathogens. As the virulence of many Gram‐negative phytopathogens is controlled by N‐acyl‐homoserine lactones (AHLs), the enzymatic disruption of this type of quorum‐sensing (QS) signal molecules, mechanism known as quorum quenching (QQ), has been proposed as a promising alternative antivirulence therapy. In this study, a novel strain of Bacillus toyonensis isolated from the halophyte plant Arthrocaulon sp. exhibited numerous traits associated with plant growth promotion (PGP) and degraded a broad range of AHLs. Three lactonases and an acylase enzymes were identified in the bacterial genome and verified in vitro. The AHL‐degrading activity of strain AA1EC1 significantly attenuated the virulence of relevant phytopathogens causing reduction of soft rot symptoms on potato and carrots. In vivo assays showed that strain AA1EC1 significantly increased plant length, stem width, root and aerial dry weights and total weight of tomato and protected plants against Pseudomonas syringae pv. tomato. To our knowledge, this is the first report to demonstrate PGP and QQ activities in the species B. toyonensis that make this strain as a promising phytostimulant and biocontrol agent. |
| first_indexed | 2024-04-24T17:36:35Z |
| format | Article |
| id | doaj.art-d21fbad589964a008d1665918b4bab6c |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-04-24T17:36:35Z |
| publishDate | 2024-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-d21fbad589964a008d1665918b4bab6c2024-03-28T04:50:35ZengWileyMicrobial Biotechnology1751-79152024-03-01173n/an/a10.1111/1751-7915.14420Potential of the quorum‐quenching and plant‐growth promoting halotolerant Bacillus toyonensis AA1EC1 as biocontrol agentAmalia Roca0Mónica Cabeo1Carlos Enguidanos2Fernando Martínez‐Checa3Inmaculada Sampedro4Inmaculada Llamas5Department of Microbiology, Faculty of Pharmacy University of Granada Granada SpainDepartment of Microbiology, Faculty of Pharmacy University of Granada Granada SpainDepartment of Microbiology, Faculty of Pharmacy University of Granada Granada SpainDepartment of Microbiology, Faculty of Pharmacy University of Granada Granada SpainDepartment of Microbiology, Faculty of Pharmacy University of Granada Granada SpainDepartment of Microbiology, Faculty of Pharmacy University of Granada Granada SpainAbstract The use of fertilizers and pesticides to control plant diseases is widespread in intensive farming causing adverse effects together with the development of antimicrobial resistance pathogens. As the virulence of many Gram‐negative phytopathogens is controlled by N‐acyl‐homoserine lactones (AHLs), the enzymatic disruption of this type of quorum‐sensing (QS) signal molecules, mechanism known as quorum quenching (QQ), has been proposed as a promising alternative antivirulence therapy. In this study, a novel strain of Bacillus toyonensis isolated from the halophyte plant Arthrocaulon sp. exhibited numerous traits associated with plant growth promotion (PGP) and degraded a broad range of AHLs. Three lactonases and an acylase enzymes were identified in the bacterial genome and verified in vitro. The AHL‐degrading activity of strain AA1EC1 significantly attenuated the virulence of relevant phytopathogens causing reduction of soft rot symptoms on potato and carrots. In vivo assays showed that strain AA1EC1 significantly increased plant length, stem width, root and aerial dry weights and total weight of tomato and protected plants against Pseudomonas syringae pv. tomato. To our knowledge, this is the first report to demonstrate PGP and QQ activities in the species B. toyonensis that make this strain as a promising phytostimulant and biocontrol agent.https://doi.org/10.1111/1751-7915.14420 |
| spellingShingle | Amalia Roca Mónica Cabeo Carlos Enguidanos Fernando Martínez‐Checa Inmaculada Sampedro Inmaculada Llamas Potential of the quorum‐quenching and plant‐growth promoting halotolerant Bacillus toyonensis AA1EC1 as biocontrol agent Microbial Biotechnology |
| title | Potential of the quorum‐quenching and plant‐growth promoting halotolerant Bacillus toyonensis AA1EC1 as biocontrol agent |
| title_full | Potential of the quorum‐quenching and plant‐growth promoting halotolerant Bacillus toyonensis AA1EC1 as biocontrol agent |
| title_fullStr | Potential of the quorum‐quenching and plant‐growth promoting halotolerant Bacillus toyonensis AA1EC1 as biocontrol agent |
| title_full_unstemmed | Potential of the quorum‐quenching and plant‐growth promoting halotolerant Bacillus toyonensis AA1EC1 as biocontrol agent |
| title_short | Potential of the quorum‐quenching and plant‐growth promoting halotolerant Bacillus toyonensis AA1EC1 as biocontrol agent |
| title_sort | potential of the quorum quenching and plant growth promoting halotolerant bacillus toyonensis aa1ec1 as biocontrol agent |
| url | https://doi.org/10.1111/1751-7915.14420 |
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