Cartilage-like protein-polysaccharide hybrid hydrogel for enhancing chondrogenic differentiation of bone marrow mesenchymal stem cells
Abstract The regeneration of articular cartilage posed a formidable challenge due to the restricted treatment efficacy of existing therapies. Scaffold-based tissue engineering emerges as a promising avenue for cartilage reconstitution. However, most scaffolds exhibit inadequate mechanical characteri...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2024-01-01
|
| Series: | Collagen and Leather |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42825-023-00146-2 |
| _version_ | 1797355536828596224 |
|---|---|
| author | Xinyue Zhang Xue Zhan Haojin Cheng Zuqin Dong Chen Hu Chenxin Liu Jie Liang Yafang Chen Yujiang Fan Xingdong Zhang |
| author_facet | Xinyue Zhang Xue Zhan Haojin Cheng Zuqin Dong Chen Hu Chenxin Liu Jie Liang Yafang Chen Yujiang Fan Xingdong Zhang |
| author_sort | Xinyue Zhang |
| collection | DOAJ |
| description | Abstract The regeneration of articular cartilage posed a formidable challenge due to the restricted treatment efficacy of existing therapies. Scaffold-based tissue engineering emerges as a promising avenue for cartilage reconstitution. However, most scaffolds exhibit inadequate mechanical characteristics, poor biocompatibility, or absent cell adhesion sites. In this study, cartilage-like protein-polysaccharide hybrid hydrogel based on DOPA-modified hyaluronic acid, bovine type I collagen (Col I), and recombinant humanized type II collagen (rhCol II), denoted as HDCR. HDCR hydrogels possessed the advantage of injectability and in situ crosslinking through pH adjustment. Moreover, HDCR hydrogels exhibited a manipulable degradation rate and favorable biocompatibility. Notably, HDCR hydrogels significantly induced chondrogenic differentiation of rabbit bone marrow mesenchymal stem cells in vitro, as demonstrated by the upregulation of crucial chondrogenic genes (type II collagen, aggrecan) and the abundant accumulation of glycosaminoglycan. This approach presented a strategy to manufacture injectable, biodegradable scaffolds based on cartilage-like protein-polysaccharide polymers, offering a minimally invasive solution for cartilage repair. Graphical abstract |
| first_indexed | 2024-03-08T14:12:34Z |
| format | Article |
| id | doaj.art-d2216b0b08a040738ed7ed123c938220 |
| institution | Directory Open Access Journal |
| issn | 2097-1419 2731-6998 |
| language | English |
| last_indexed | 2024-03-08T14:12:34Z |
| publishDate | 2024-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Collagen and Leather |
| spelling | doaj.art-d2216b0b08a040738ed7ed123c9382202024-01-14T12:36:25ZengSpringerOpenCollagen and Leather2097-14192731-69982024-01-016111210.1186/s42825-023-00146-2Cartilage-like protein-polysaccharide hybrid hydrogel for enhancing chondrogenic differentiation of bone marrow mesenchymal stem cellsXinyue Zhang0Xue Zhan1Haojin Cheng2Zuqin Dong3Chen Hu4Chenxin Liu5Jie Liang6Yafang Chen7Yujiang Fan8Xingdong Zhang9National Engineering Research Center for Biomaterials, Sichuan UniversityNational Engineering Research Center for Biomaterials, Sichuan UniversityNational Engineering Research Center for Biomaterials, Sichuan UniversityNational Engineering Research Center for Biomaterials, Sichuan UniversityNational Engineering Research Center for Biomaterials, Sichuan UniversityNational Engineering Research Center for Biomaterials, Sichuan UniversityNational Engineering Research Center for Biomaterials, Sichuan UniversityNational Engineering Research Center for Biomaterials, Sichuan UniversityNational Engineering Research Center for Biomaterials, Sichuan UniversityNational Engineering Research Center for Biomaterials, Sichuan UniversityAbstract The regeneration of articular cartilage posed a formidable challenge due to the restricted treatment efficacy of existing therapies. Scaffold-based tissue engineering emerges as a promising avenue for cartilage reconstitution. However, most scaffolds exhibit inadequate mechanical characteristics, poor biocompatibility, or absent cell adhesion sites. In this study, cartilage-like protein-polysaccharide hybrid hydrogel based on DOPA-modified hyaluronic acid, bovine type I collagen (Col I), and recombinant humanized type II collagen (rhCol II), denoted as HDCR. HDCR hydrogels possessed the advantage of injectability and in situ crosslinking through pH adjustment. Moreover, HDCR hydrogels exhibited a manipulable degradation rate and favorable biocompatibility. Notably, HDCR hydrogels significantly induced chondrogenic differentiation of rabbit bone marrow mesenchymal stem cells in vitro, as demonstrated by the upregulation of crucial chondrogenic genes (type II collagen, aggrecan) and the abundant accumulation of glycosaminoglycan. This approach presented a strategy to manufacture injectable, biodegradable scaffolds based on cartilage-like protein-polysaccharide polymers, offering a minimally invasive solution for cartilage repair. Graphical abstracthttps://doi.org/10.1186/s42825-023-00146-2Chondrogenic differentiationRecombinant humanized collagenHyaluronic acidDopamine hydrochlorideArticular cartilage |
| spellingShingle | Xinyue Zhang Xue Zhan Haojin Cheng Zuqin Dong Chen Hu Chenxin Liu Jie Liang Yafang Chen Yujiang Fan Xingdong Zhang Cartilage-like protein-polysaccharide hybrid hydrogel for enhancing chondrogenic differentiation of bone marrow mesenchymal stem cells Collagen and Leather Chondrogenic differentiation Recombinant humanized collagen Hyaluronic acid Dopamine hydrochloride Articular cartilage |
| title | Cartilage-like protein-polysaccharide hybrid hydrogel for enhancing chondrogenic differentiation of bone marrow mesenchymal stem cells |
| title_full | Cartilage-like protein-polysaccharide hybrid hydrogel for enhancing chondrogenic differentiation of bone marrow mesenchymal stem cells |
| title_fullStr | Cartilage-like protein-polysaccharide hybrid hydrogel for enhancing chondrogenic differentiation of bone marrow mesenchymal stem cells |
| title_full_unstemmed | Cartilage-like protein-polysaccharide hybrid hydrogel for enhancing chondrogenic differentiation of bone marrow mesenchymal stem cells |
| title_short | Cartilage-like protein-polysaccharide hybrid hydrogel for enhancing chondrogenic differentiation of bone marrow mesenchymal stem cells |
| title_sort | cartilage like protein polysaccharide hybrid hydrogel for enhancing chondrogenic differentiation of bone marrow mesenchymal stem cells |
| topic | Chondrogenic differentiation Recombinant humanized collagen Hyaluronic acid Dopamine hydrochloride Articular cartilage |
| url | https://doi.org/10.1186/s42825-023-00146-2 |
| work_keys_str_mv | AT xinyuezhang cartilagelikeproteinpolysaccharidehybridhydrogelforenhancingchondrogenicdifferentiationofbonemarrowmesenchymalstemcells AT xuezhan cartilagelikeproteinpolysaccharidehybridhydrogelforenhancingchondrogenicdifferentiationofbonemarrowmesenchymalstemcells AT haojincheng cartilagelikeproteinpolysaccharidehybridhydrogelforenhancingchondrogenicdifferentiationofbonemarrowmesenchymalstemcells AT zuqindong cartilagelikeproteinpolysaccharidehybridhydrogelforenhancingchondrogenicdifferentiationofbonemarrowmesenchymalstemcells AT chenhu cartilagelikeproteinpolysaccharidehybridhydrogelforenhancingchondrogenicdifferentiationofbonemarrowmesenchymalstemcells AT chenxinliu cartilagelikeproteinpolysaccharidehybridhydrogelforenhancingchondrogenicdifferentiationofbonemarrowmesenchymalstemcells AT jieliang cartilagelikeproteinpolysaccharidehybridhydrogelforenhancingchondrogenicdifferentiationofbonemarrowmesenchymalstemcells AT yafangchen cartilagelikeproteinpolysaccharidehybridhydrogelforenhancingchondrogenicdifferentiationofbonemarrowmesenchymalstemcells AT yujiangfan cartilagelikeproteinpolysaccharidehybridhydrogelforenhancingchondrogenicdifferentiationofbonemarrowmesenchymalstemcells AT xingdongzhang cartilagelikeproteinpolysaccharidehybridhydrogelforenhancingchondrogenicdifferentiationofbonemarrowmesenchymalstemcells |