Effect of Temperature and Ionic Substitutions on the Tegumental Potentials of Protoscoleces of <i>Echinococcus granulosus</i>
The protoscolex (PSC) is generated by asexual reproduction at the larval stage of taeniid <i>Echinococcus granulosus</i> that causes cystic echinococcosis or hydatidosis, a worldwide zoonosis. The PSC is enveloped by a complex cellular syncytial tegument responsible for ionic movements a...
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MDPI AG
2023-06-01
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| Series: | Tropical Medicine and Infectious Disease |
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| Online Access: | https://www.mdpi.com/2414-6366/8/6/303 |
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| author | Mónica Patricia Antonella Carabajal María José Fernández Salom Santiago Olivera Horacio F. Cantiello |
| author_facet | Mónica Patricia Antonella Carabajal María José Fernández Salom Santiago Olivera Horacio F. Cantiello |
| author_sort | Mónica Patricia Antonella Carabajal |
| collection | DOAJ |
| description | The protoscolex (PSC) is generated by asexual reproduction at the larval stage of taeniid <i>Echinococcus granulosus</i> that causes cystic echinococcosis or hydatidosis, a worldwide zoonosis. The PSC is enveloped by a complex cellular syncytial tegument responsible for ionic movements and the hydroelectrolytic balance of the parasite. We recently reported on two electrical potentials in bovine lung protoscoleces (PSCs) that reflect differences in ionic movements between the parasite’s invaginated and evaginated developmental stages. Here, we explored the effect of temperature and ionic substitutions on the tegumental potentials of bovine lung PSCs of <i>Echinococcus granulosus</i> by microelectrode impalements. We observed that the transient peak potential was temperature-dependent, consistent with an active transport component in the invaginated state only. Further changes in the electrical potentials by high K<sup>+</sup> depolarization, low external Ca<sup>2+</sup>, and addition of the diuretic amiloride are in agreement with the presence of a Ca<sup>2+</sup>-sensitive cation-selective electrodiffusional pathway in the outer surface of the parasite. Variations in electrical potential differences through the tegument provide an accessible and valuable parameter for studying ionic transport mechanisms and, therefore, potential targets for developing novel antiparasitic drugs. |
| first_indexed | 2024-03-11T01:52:31Z |
| format | Article |
| id | doaj.art-d22350bb74e247a8a3ac1c14a704f5fa |
| institution | Directory Open Access Journal |
| issn | 2414-6366 |
| language | English |
| last_indexed | 2024-03-11T01:52:31Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Tropical Medicine and Infectious Disease |
| spelling | doaj.art-d22350bb74e247a8a3ac1c14a704f5fa2023-11-18T12:56:17ZengMDPI AGTropical Medicine and Infectious Disease2414-63662023-06-018630310.3390/tropicalmed8060303Effect of Temperature and Ionic Substitutions on the Tegumental Potentials of Protoscoleces of <i>Echinococcus granulosus</i>Mónica Patricia Antonella Carabajal0María José Fernández Salom1Santiago Olivera2Horacio F. Cantiello3Laboratorio de Canales Iónicos, Instituto Multidisciplinario de Salud, Tecnología y Desarrollo (IMSaTeD, CONICET-UNSE), Santiago del Estero 4206, ArgentinaLaboratorio de Canales Iónicos, Instituto Multidisciplinario de Salud, Tecnología y Desarrollo (IMSaTeD, CONICET-UNSE), Santiago del Estero 4206, ArgentinaLaboratorio de Canales Iónicos, Instituto Multidisciplinario de Salud, Tecnología y Desarrollo (IMSaTeD, CONICET-UNSE), Santiago del Estero 4206, ArgentinaLaboratorio de Canales Iónicos, Instituto Multidisciplinario de Salud, Tecnología y Desarrollo (IMSaTeD, CONICET-UNSE), Santiago del Estero 4206, ArgentinaThe protoscolex (PSC) is generated by asexual reproduction at the larval stage of taeniid <i>Echinococcus granulosus</i> that causes cystic echinococcosis or hydatidosis, a worldwide zoonosis. The PSC is enveloped by a complex cellular syncytial tegument responsible for ionic movements and the hydroelectrolytic balance of the parasite. We recently reported on two electrical potentials in bovine lung protoscoleces (PSCs) that reflect differences in ionic movements between the parasite’s invaginated and evaginated developmental stages. Here, we explored the effect of temperature and ionic substitutions on the tegumental potentials of bovine lung PSCs of <i>Echinococcus granulosus</i> by microelectrode impalements. We observed that the transient peak potential was temperature-dependent, consistent with an active transport component in the invaginated state only. Further changes in the electrical potentials by high K<sup>+</sup> depolarization, low external Ca<sup>2+</sup>, and addition of the diuretic amiloride are in agreement with the presence of a Ca<sup>2+</sup>-sensitive cation-selective electrodiffusional pathway in the outer surface of the parasite. Variations in electrical potential differences through the tegument provide an accessible and valuable parameter for studying ionic transport mechanisms and, therefore, potential targets for developing novel antiparasitic drugs.https://www.mdpi.com/2414-6366/8/6/303<i>E. granulosus</i>tegumental potentialactive transportelectrodiffusional ion movement |
| spellingShingle | Mónica Patricia Antonella Carabajal María José Fernández Salom Santiago Olivera Horacio F. Cantiello Effect of Temperature and Ionic Substitutions on the Tegumental Potentials of Protoscoleces of <i>Echinococcus granulosus</i> Tropical Medicine and Infectious Disease <i>E. granulosus</i> tegumental potential active transport electrodiffusional ion movement |
| title | Effect of Temperature and Ionic Substitutions on the Tegumental Potentials of Protoscoleces of <i>Echinococcus granulosus</i> |
| title_full | Effect of Temperature and Ionic Substitutions on the Tegumental Potentials of Protoscoleces of <i>Echinococcus granulosus</i> |
| title_fullStr | Effect of Temperature and Ionic Substitutions on the Tegumental Potentials of Protoscoleces of <i>Echinococcus granulosus</i> |
| title_full_unstemmed | Effect of Temperature and Ionic Substitutions on the Tegumental Potentials of Protoscoleces of <i>Echinococcus granulosus</i> |
| title_short | Effect of Temperature and Ionic Substitutions on the Tegumental Potentials of Protoscoleces of <i>Echinococcus granulosus</i> |
| title_sort | effect of temperature and ionic substitutions on the tegumental potentials of protoscoleces of i echinococcus granulosus i |
| topic | <i>E. granulosus</i> tegumental potential active transport electrodiffusional ion movement |
| url | https://www.mdpi.com/2414-6366/8/6/303 |
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