Transcriptome profiling of Staphylococci-infected cow mammary gland parenchyma
Abstract Background Genome-wide gene expression profiling allows for identification of genes involved in the defense response of the host against pathogens. As presented here, transcriptomic analysis and bioinformatics tools were applied in order to identify genes expressed in the mammary gland pare...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-06-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12917-017-1088-2 |
| _version_ | 1819139084685148160 |
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| author | Ewa M Kosciuczuk Paweł Lisowski Justyna Jarczak Alicja Majewska Magdalena Rzewuska Lech Zwierzchowski Emilia Bagnicka |
| author_facet | Ewa M Kosciuczuk Paweł Lisowski Justyna Jarczak Alicja Majewska Magdalena Rzewuska Lech Zwierzchowski Emilia Bagnicka |
| author_sort | Ewa M Kosciuczuk |
| collection | DOAJ |
| description | Abstract Background Genome-wide gene expression profiling allows for identification of genes involved in the defense response of the host against pathogens. As presented here, transcriptomic analysis and bioinformatics tools were applied in order to identify genes expressed in the mammary gland parenchyma of cows naturally infected with coagulase-positive and coagulase-negative Staphylococci. Results In cows infected with coagulase-positive Staphylococci, being in 1st or 2nd lactation, 1700 differentially expressed genes (DEGs) were identified. However, examination of the 3rd or 4th lactations revealed 2200 DEGs. Gene ontology functional classification showed the molecular functions of the DEGs overrepresented the activity of cytokines, chemokines, and their receptors. In cows infected with coagulase-negative Staphylococci, in the 1st or 2nd lactations 418 DEGs, while in the 3rd or 4th lactations, 1200 DEGs were identified that involved in molecular functions such as protein, calcium ion and lipid binding, chemokine activity, and protein homodimerization. Gene network analysis showed DEGs associated with inflammation, cell migration, and immune response to infection, development of cells and tissues, and humoral responses to infections caused by both types of Staphylococci. Conclusion A coagulase-positive Staphylococci infection caused a markedly stronger host response than that of coagulase-negative, resulting in vastly increased DEGs. A significant increase in the expression of the FOS, TNF, and genes encoding the major histocompatibility complex proteins (MHC) was observed. It suggests these genes play a key role in the synchronization of the immune response of the cow’s parenchyma against mastitis-causing bacteria. Moreover, the following genes that belong to several physiological pathways (KEGG pathways) were selected for further studies as candidate genes of mammary gland immune response for use in Marker Assisted Selection (MAS): chemokine signaling pathway (CCL2, CXCL5, HCK, CCR1), cell adhesion molecules (CAMs) pathway (BOLA-DQA2, BOLA-DQA1, F11R, ITGAL, CD86), antigen processing and presentation pathway (CD8A, PDIA3, LGMN, IFI30, HSPA1A), and NOD-like receptor signaling pathway (TNF, IL8, IL18, NFKBIA). |
| first_indexed | 2024-12-22T11:17:03Z |
| format | Article |
| id | doaj.art-d22429f4bf134302a1cfc6066a0eecea |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-22T11:17:03Z |
| publishDate | 2017-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-d22429f4bf134302a1cfc6066a0eecea2022-12-21T18:27:58ZengBMCBMC Veterinary Research1746-61482017-06-0113111210.1186/s12917-017-1088-2Transcriptome profiling of Staphylococci-infected cow mammary gland parenchymaEwa M Kosciuczuk0Paweł Lisowski1Justyna Jarczak2Alicja Majewska3Magdalena Rzewuska4Lech Zwierzchowski5Emilia Bagnicka6Department of Animal Improvement, Institute of Genetics and Animal Breeding Polish Academy of SciencesDepartment of Animal Improvement, Institute of Genetics and Animal Breeding Polish Academy of SciencesDepartment of Animal Improvement, Institute of Genetics and Animal Breeding Polish Academy of SciencesDepartment of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life SciencesDepartment of Pre-Clinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life SciencesDepartment of Animal Improvement, Institute of Genetics and Animal Breeding Polish Academy of SciencesDepartment of Animal Improvement, Institute of Genetics and Animal Breeding Polish Academy of SciencesAbstract Background Genome-wide gene expression profiling allows for identification of genes involved in the defense response of the host against pathogens. As presented here, transcriptomic analysis and bioinformatics tools were applied in order to identify genes expressed in the mammary gland parenchyma of cows naturally infected with coagulase-positive and coagulase-negative Staphylococci. Results In cows infected with coagulase-positive Staphylococci, being in 1st or 2nd lactation, 1700 differentially expressed genes (DEGs) were identified. However, examination of the 3rd or 4th lactations revealed 2200 DEGs. Gene ontology functional classification showed the molecular functions of the DEGs overrepresented the activity of cytokines, chemokines, and their receptors. In cows infected with coagulase-negative Staphylococci, in the 1st or 2nd lactations 418 DEGs, while in the 3rd or 4th lactations, 1200 DEGs were identified that involved in molecular functions such as protein, calcium ion and lipid binding, chemokine activity, and protein homodimerization. Gene network analysis showed DEGs associated with inflammation, cell migration, and immune response to infection, development of cells and tissues, and humoral responses to infections caused by both types of Staphylococci. Conclusion A coagulase-positive Staphylococci infection caused a markedly stronger host response than that of coagulase-negative, resulting in vastly increased DEGs. A significant increase in the expression of the FOS, TNF, and genes encoding the major histocompatibility complex proteins (MHC) was observed. It suggests these genes play a key role in the synchronization of the immune response of the cow’s parenchyma against mastitis-causing bacteria. Moreover, the following genes that belong to several physiological pathways (KEGG pathways) were selected for further studies as candidate genes of mammary gland immune response for use in Marker Assisted Selection (MAS): chemokine signaling pathway (CCL2, CXCL5, HCK, CCR1), cell adhesion molecules (CAMs) pathway (BOLA-DQA2, BOLA-DQA1, F11R, ITGAL, CD86), antigen processing and presentation pathway (CD8A, PDIA3, LGMN, IFI30, HSPA1A), and NOD-like receptor signaling pathway (TNF, IL8, IL18, NFKBIA).http://link.springer.com/article/10.1186/s12917-017-1088-2Dairy cowsUdder parenchymaChronic mastitisTranscriptomicsGene expression profilingmicroarray |
| spellingShingle | Ewa M Kosciuczuk Paweł Lisowski Justyna Jarczak Alicja Majewska Magdalena Rzewuska Lech Zwierzchowski Emilia Bagnicka Transcriptome profiling of Staphylococci-infected cow mammary gland parenchyma BMC Veterinary Research Dairy cows Udder parenchyma Chronic mastitis Transcriptomics Gene expression profiling microarray |
| title | Transcriptome profiling of Staphylococci-infected cow mammary gland parenchyma |
| title_full | Transcriptome profiling of Staphylococci-infected cow mammary gland parenchyma |
| title_fullStr | Transcriptome profiling of Staphylococci-infected cow mammary gland parenchyma |
| title_full_unstemmed | Transcriptome profiling of Staphylococci-infected cow mammary gland parenchyma |
| title_short | Transcriptome profiling of Staphylococci-infected cow mammary gland parenchyma |
| title_sort | transcriptome profiling of staphylococci infected cow mammary gland parenchyma |
| topic | Dairy cows Udder parenchyma Chronic mastitis Transcriptomics Gene expression profiling microarray |
| url | http://link.springer.com/article/10.1186/s12917-017-1088-2 |
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