Identification of major QTLs for yield-related traits with improved genetic map in wheat
IntroductionIdentification of stable major quantitative trait loci (QTLs) for yield-related traits is important for yield potential improvement in wheat breeding.MethodsIn the present study, we genotyped a recombinant inbred line (RIL) population using the Wheat 660K SNP array and constructed a high...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1138696/full |
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| author | Feifei Ma Yunfeng Xu Ruifang Wang Yiping Tong Aimin Zhang Aimin Zhang Dongcheng Liu Diaoguo An |
| author_facet | Feifei Ma Yunfeng Xu Ruifang Wang Yiping Tong Aimin Zhang Aimin Zhang Dongcheng Liu Diaoguo An |
| author_sort | Feifei Ma |
| collection | DOAJ |
| description | IntroductionIdentification of stable major quantitative trait loci (QTLs) for yield-related traits is important for yield potential improvement in wheat breeding.MethodsIn the present study, we genotyped a recombinant inbred line (RIL) population using the Wheat 660K SNP array and constructed a high-density genetic map. The genetic map showed high collinearity with the wheat genome assembly. Fourteen yield-related traits were evaluated in six environments for QTL analysis.Results and DiscussionA total of 12 environmentally stable QTLs were identified in at least three environments, explaining up to 34.7% of the phenotypic variation. Of these, QTkw-1B.2 for thousand kernel weight (TKW), QPh-2D.1 (QSl-2D.2/QScn-2D.1) for plant height (PH), spike length (SL) and spikelet compactness (SCN), QPh-4B.1 for PH, and QTss-7A.3 for total spikelet number per spike (TSS) were detected in at least five environments. A set of Kompetitive Allele Specific PCR (KASP) markers were converted based on the above QTLs and used to genotype a diversity panel comprising of 190 wheat accessions across four growing seasons. QPh-2D.1 (QSl-2D.2/QScn-2D.1), QPh-4B.1 and QTss-7A.3 were successfully validated. Compared with previous studies, QTkw-1B.2 and QPh-4B.1 should be novel QTLs. These results provided a solid foundation for further positional cloning and marker-assisted selection of the targeted QTLs in wheat breeding programs. |
| first_indexed | 2024-04-09T23:52:56Z |
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| language | English |
| last_indexed | 2024-04-09T23:52:56Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-d2287e784b2b41cba20f6bae9de227312023-03-17T05:00:26ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-03-011410.3389/fpls.2023.11386961138696Identification of major QTLs for yield-related traits with improved genetic map in wheatFeifei Ma0Yunfeng Xu1Ruifang Wang2Yiping Tong3Aimin Zhang4Aimin Zhang5Dongcheng Liu6Diaoguo An7Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of North China Crop Improvement and Regulation, College of Agronomy, Hebei Agricultural University, Baoding, Hebei, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of North China Crop Improvement and Regulation, College of Agronomy, Hebei Agricultural University, Baoding, Hebei, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, ChinaIntroductionIdentification of stable major quantitative trait loci (QTLs) for yield-related traits is important for yield potential improvement in wheat breeding.MethodsIn the present study, we genotyped a recombinant inbred line (RIL) population using the Wheat 660K SNP array and constructed a high-density genetic map. The genetic map showed high collinearity with the wheat genome assembly. Fourteen yield-related traits were evaluated in six environments for QTL analysis.Results and DiscussionA total of 12 environmentally stable QTLs were identified in at least three environments, explaining up to 34.7% of the phenotypic variation. Of these, QTkw-1B.2 for thousand kernel weight (TKW), QPh-2D.1 (QSl-2D.2/QScn-2D.1) for plant height (PH), spike length (SL) and spikelet compactness (SCN), QPh-4B.1 for PH, and QTss-7A.3 for total spikelet number per spike (TSS) were detected in at least five environments. A set of Kompetitive Allele Specific PCR (KASP) markers were converted based on the above QTLs and used to genotype a diversity panel comprising of 190 wheat accessions across four growing seasons. QPh-2D.1 (QSl-2D.2/QScn-2D.1), QPh-4B.1 and QTss-7A.3 were successfully validated. Compared with previous studies, QTkw-1B.2 and QPh-4B.1 should be novel QTLs. These results provided a solid foundation for further positional cloning and marker-assisted selection of the targeted QTLs in wheat breeding programs.https://www.frontiersin.org/articles/10.3389/fpls.2023.1138696/fullwheathigh-density linkage mapQTL mappingyield-related traitsthe wheat 660K SNP array |
| spellingShingle | Feifei Ma Yunfeng Xu Ruifang Wang Yiping Tong Aimin Zhang Aimin Zhang Dongcheng Liu Diaoguo An Identification of major QTLs for yield-related traits with improved genetic map in wheat Frontiers in Plant Science wheat high-density linkage map QTL mapping yield-related traits the wheat 660K SNP array |
| title | Identification of major QTLs for yield-related traits with improved genetic map in wheat |
| title_full | Identification of major QTLs for yield-related traits with improved genetic map in wheat |
| title_fullStr | Identification of major QTLs for yield-related traits with improved genetic map in wheat |
| title_full_unstemmed | Identification of major QTLs for yield-related traits with improved genetic map in wheat |
| title_short | Identification of major QTLs for yield-related traits with improved genetic map in wheat |
| title_sort | identification of major qtls for yield related traits with improved genetic map in wheat |
| topic | wheat high-density linkage map QTL mapping yield-related traits the wheat 660K SNP array |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1138696/full |
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