miR-124 and VAMP3 Act Antagonistically in Human Neuroblastoma
Neuroblastoma (NB) is the most common extracranial solid tumor that affects developing nerve cells in the fetus, infants, and children. miR-124 is a microRNA (miRNA) enriched in neuronal tissues, and VAMP3 (vesicle-associated membrane protein 3) has been reported to be an miR-124 target, although th...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/19/14877 |
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| author | Xiaoxiao Zhang Chengyong Yang Zhen Meng Huanhuan Zhong Xutian Hou Fenfen Wang Yiping Lu Jingjing Guo Yan Zeng |
| author_facet | Xiaoxiao Zhang Chengyong Yang Zhen Meng Huanhuan Zhong Xutian Hou Fenfen Wang Yiping Lu Jingjing Guo Yan Zeng |
| author_sort | Xiaoxiao Zhang |
| collection | DOAJ |
| description | Neuroblastoma (NB) is the most common extracranial solid tumor that affects developing nerve cells in the fetus, infants, and children. miR-124 is a microRNA (miRNA) enriched in neuronal tissues, and VAMP3 (vesicle-associated membrane protein 3) has been reported to be an miR-124 target, although the relationship between NB and miR-124 or VAMP3 is unknown. Our current work identified that miR-124 levels are high in NB cases and that elevated miR-124 correlates with worse NB outcomes. Conversely, depressed VAMP3 correlates with worse NB outcomes. To investigate the mechanisms by which miR-124 and VAMP3 regulate NB, we altered miR-124 or VAMP3 expression in human NB cells and observed that increased miR-124 and reduced VAMP3 stimulated cell proliferation and suppressed apoptosis, while increased VAMP3 had the opposite effects. Genome-wide mRNA expression analyses identified gene and pathway changes which might explain the NB cell phenotypes. Together, our studies suggest that miR-124 and VAMP3 could be potential new markers of NB and targets of NB treatments. |
| first_indexed | 2024-03-10T21:43:08Z |
| format | Article |
| id | doaj.art-d22a5a378d2745f68753d8d187bd069e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T21:43:08Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d22a5a378d2745f68753d8d187bd069e2023-11-19T14:31:58ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124191487710.3390/ijms241914877miR-124 and VAMP3 Act Antagonistically in Human NeuroblastomaXiaoxiao Zhang0Chengyong Yang1Zhen Meng2Huanhuan Zhong3Xutian Hou4Fenfen Wang5Yiping Lu6Jingjing Guo7Yan Zeng8Department of Zoology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Zoology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Zoology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Zoology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Zoology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Zoology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Zoology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaCentre in Artificial Intelligence Driven Drug Discovery, Faculty of Applied Sciences, Macao Polytechnic University, Macao 999078, ChinaDepartment of Zoology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaNeuroblastoma (NB) is the most common extracranial solid tumor that affects developing nerve cells in the fetus, infants, and children. miR-124 is a microRNA (miRNA) enriched in neuronal tissues, and VAMP3 (vesicle-associated membrane protein 3) has been reported to be an miR-124 target, although the relationship between NB and miR-124 or VAMP3 is unknown. Our current work identified that miR-124 levels are high in NB cases and that elevated miR-124 correlates with worse NB outcomes. Conversely, depressed VAMP3 correlates with worse NB outcomes. To investigate the mechanisms by which miR-124 and VAMP3 regulate NB, we altered miR-124 or VAMP3 expression in human NB cells and observed that increased miR-124 and reduced VAMP3 stimulated cell proliferation and suppressed apoptosis, while increased VAMP3 had the opposite effects. Genome-wide mRNA expression analyses identified gene and pathway changes which might explain the NB cell phenotypes. Together, our studies suggest that miR-124 and VAMP3 could be potential new markers of NB and targets of NB treatments.https://www.mdpi.com/1422-0067/24/19/14877miR-124VAMP3neuroblastomaapoptosisgene expression |
| spellingShingle | Xiaoxiao Zhang Chengyong Yang Zhen Meng Huanhuan Zhong Xutian Hou Fenfen Wang Yiping Lu Jingjing Guo Yan Zeng miR-124 and VAMP3 Act Antagonistically in Human Neuroblastoma International Journal of Molecular Sciences miR-124 VAMP3 neuroblastoma apoptosis gene expression |
| title | miR-124 and VAMP3 Act Antagonistically in Human Neuroblastoma |
| title_full | miR-124 and VAMP3 Act Antagonistically in Human Neuroblastoma |
| title_fullStr | miR-124 and VAMP3 Act Antagonistically in Human Neuroblastoma |
| title_full_unstemmed | miR-124 and VAMP3 Act Antagonistically in Human Neuroblastoma |
| title_short | miR-124 and VAMP3 Act Antagonistically in Human Neuroblastoma |
| title_sort | mir 124 and vamp3 act antagonistically in human neuroblastoma |
| topic | miR-124 VAMP3 neuroblastoma apoptosis gene expression |
| url | https://www.mdpi.com/1422-0067/24/19/14877 |
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