Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies
Fourteen hepatitis C virus (HCV) chronically infected patients were submitted to routine liver biopsy for histological evaluation. Liver samples were assayed to HCV-RNA by in situ hybridization, using digoxigenin labeled probe. HCV genotypes were found to be predominantly type 1 (71.4%), followed by...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2005-05-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000300009 |
| _version_ | 1797718472363343872 |
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| author | HS Carvalho ML Baptista MA Pinto M Silva CM Takiya V Chagas V Pannain HSM Coelho CFT Yoshida |
| author_facet | HS Carvalho ML Baptista MA Pinto M Silva CM Takiya V Chagas V Pannain HSM Coelho CFT Yoshida |
| author_sort | HS Carvalho |
| collection | DOAJ |
| description | Fourteen hepatitis C virus (HCV) chronically infected patients were submitted to routine liver biopsy for histological evaluation. Liver samples were assayed to HCV-RNA by in situ hybridization, using digoxigenin labeled probe. HCV genotypes were found to be predominantly type 1 (71.4%), followed by genotype 3 (21.4%), and genotype 2 (7.2%). Alanine-aminotransferase levels were raised in 10 patients. The histopathological scores were minimal (21.4%), mild (57.2%), and moderate (21.4%). Viral RNA was detected in liver cells from nine patients (64.3%). ISH method provides localization and poor confirmation of HCV RNA in the liver tissue of HCV chronic patients. |
| first_indexed | 2024-03-12T08:52:18Z |
| format | Article |
| id | doaj.art-d22f4028465b4cd4a067f9b17de47e53 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:52:18Z |
| publishDate | 2005-05-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-d22f4028465b4cd4a067f9b17de47e532023-09-02T16:17:53ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602005-05-01100326927210.1590/S0074-02762005000300009Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsiesHS CarvalhoML BaptistaMA PintoM SilvaCM TakiyaV ChagasV PannainHSM CoelhoCFT YoshidaFourteen hepatitis C virus (HCV) chronically infected patients were submitted to routine liver biopsy for histological evaluation. Liver samples were assayed to HCV-RNA by in situ hybridization, using digoxigenin labeled probe. HCV genotypes were found to be predominantly type 1 (71.4%), followed by genotype 3 (21.4%), and genotype 2 (7.2%). Alanine-aminotransferase levels were raised in 10 patients. The histopathological scores were minimal (21.4%), mild (57.2%), and moderate (21.4%). Viral RNA was detected in liver cells from nine patients (64.3%). ISH method provides localization and poor confirmation of HCV RNA in the liver tissue of HCV chronic patients.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000300009hepatitis C virus RNAin situ hybridizationliver biopsychronic hepatitisviral hepatitis |
| spellingShingle | HS Carvalho ML Baptista MA Pinto M Silva CM Takiya V Chagas V Pannain HSM Coelho CFT Yoshida Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies Memorias do Instituto Oswaldo Cruz hepatitis C virus RNA in situ hybridization liver biopsy chronic hepatitis viral hepatitis |
| title | Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies |
| title_full | Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies |
| title_fullStr | Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies |
| title_full_unstemmed | Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies |
| title_short | Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies |
| title_sort | detection of hepatitis c virus rna by in situ hybridization in paraformaldehyde fixed biopsies |
| topic | hepatitis C virus RNA in situ hybridization liver biopsy chronic hepatitis viral hepatitis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000300009 |
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