A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
Isothermal DNA amplification techniques are useful for diagnostic applications in place of traditional PCR. Here the authors describe CRISDA, which combines CRISPR–Cas9 with strand displacement amplification and exhibits attomolar sensitivity and single-nucleotide specificity in DNA detection.
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2018-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-018-07324-5 |
| _version_ | 1819040137860874240 |
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| author | Wenhua Zhou Li Hu Liming Ying Zhen Zhao Paul K. Chu Xue-Feng Yu |
| author_facet | Wenhua Zhou Li Hu Liming Ying Zhen Zhao Paul K. Chu Xue-Feng Yu |
| author_sort | Wenhua Zhou |
| collection | DOAJ |
| description | Isothermal DNA amplification techniques are useful for diagnostic applications in place of traditional PCR. Here the authors describe CRISDA, which combines CRISPR–Cas9 with strand displacement amplification and exhibits attomolar sensitivity and single-nucleotide specificity in DNA detection. |
| first_indexed | 2024-12-21T09:04:19Z |
| format | Article |
| id | doaj.art-d237beec5d754883864eedecfd2f71be |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-21T09:04:19Z |
| publishDate | 2018-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-d237beec5d754883864eedecfd2f71be2022-12-21T19:09:23ZengNature PortfolioNature Communications2041-17232018-11-019111110.1038/s41467-018-07324-5A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detectionWenhua Zhou0Li Hu1Liming Ying2Zhen Zhao3Paul K. Chu4Xue-Feng Yu5Center for Biomedical Materials and Interfaces, Shenzhen Institutes of Advanced Technology, Chinese Academy of SciencesCenter for Biomedical Materials and Interfaces, Shenzhen Institutes of Advanced Technology, Chinese Academy of SciencesFaculty of Medicine, Molecular Medicine, National Heart and Lung Institute, Imperial College LondonCenter for Biomedical Materials and Interfaces, Shenzhen Institutes of Advanced Technology, Chinese Academy of SciencesDepartment of Physics, City University of Hong KongCenter for Biomedical Materials and Interfaces, Shenzhen Institutes of Advanced Technology, Chinese Academy of SciencesIsothermal DNA amplification techniques are useful for diagnostic applications in place of traditional PCR. Here the authors describe CRISDA, which combines CRISPR–Cas9 with strand displacement amplification and exhibits attomolar sensitivity and single-nucleotide specificity in DNA detection.https://doi.org/10.1038/s41467-018-07324-5 |
| spellingShingle | Wenhua Zhou Li Hu Liming Ying Zhen Zhao Paul K. Chu Xue-Feng Yu A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection Nature Communications |
| title | A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection |
| title_full | A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection |
| title_fullStr | A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection |
| title_full_unstemmed | A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection |
| title_short | A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection |
| title_sort | crispr cas9 triggered strand displacement amplification method for ultrasensitive dna detection |
| url | https://doi.org/10.1038/s41467-018-07324-5 |
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