Which incision is better for Lewis to Brown Norway rat liver transplantation, transverse or midline?
Orthotopic rat liver transplantation (OLT) is a complex microsurgical procedure extensively applied to basic science, myriad complications can occur, but incision-related self-biting has not been reported after OLT. For the project of tolerance induction through stem cells, we performed OLT from Lew...
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Elsevier
2023-07-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402305421X |
| _version_ | 1797771457397260288 |
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| author | Gaofeng Tang Huibo Zhao Guoyong Chen Shaotang Zhou |
| author_facet | Gaofeng Tang Huibo Zhao Guoyong Chen Shaotang Zhou |
| author_sort | Gaofeng Tang |
| collection | DOAJ |
| description | Orthotopic rat liver transplantation (OLT) is a complex microsurgical procedure extensively applied to basic science, myriad complications can occur, but incision-related self-biting has not been reported after OLT. For the project of tolerance induction through stem cells, we performed OLT from Lewis to Brown Norway (BN) rats as an acute rejection model and divided the study was into the transverse incision group (n = 15) and midline incision group (n = 22), while cyclosporine A was subcutaneously injected for 10-day immunosuppression use, lidocaine cream was used for pain-relieving. The recipient survival and wound status were the primary endpoint of this study. For the transverse incision group, 30-day survival rate was 40% (6/15), self-biting occurred in 13 cases in 7–39 days, the degree 1 of biting occurred in 1 cases, the degree 2 in 2 cases. The degree 3 in 10 cases, which caused death or euthanasia, the self-biting rate was 86.7% (13/15), For the midline incision group, 30-day survival rate was 100% (22/22), the degree 1 of self-biting occurred in 3 cases, no severe self-biting occurred. There were significant differences for survival (p = 0.0003) and for self-biting rate (p < 0.01) between two groups. In conclusion, incision-related self-biting behavior occurs due to incisional injury, the transverse incision is severely pain-causing; the midline one is effective to avert occurrences. |
| first_indexed | 2024-03-12T21:36:55Z |
| format | Article |
| id | doaj.art-d23a052e0c5047138c6cacab87878e67 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-12T21:36:55Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-d23a052e0c5047138c6cacab87878e672023-07-27T05:58:55ZengElsevierHeliyon2405-84402023-07-0197e18213Which incision is better for Lewis to Brown Norway rat liver transplantation, transverse or midline?Gaofeng Tang0Huibo Zhao1Guoyong Chen2Shaotang Zhou36th Hepatopancreaticobiliary Surgery of Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, 7 weiwu Road, Jinshui district, Zhengzhou, Henan, 450003, China6th Hepatopancreaticobiliary Surgery of Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, 7 weiwu Road, Jinshui district, Zhengzhou, Henan, 450003, ChinaCorresponding author.; 6th Hepatopancreaticobiliary Surgery of Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, 7 weiwu Road, Jinshui district, Zhengzhou, Henan, 450003, ChinaCorresponding author.; 6th Hepatopancreaticobiliary Surgery of Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, 7 weiwu Road, Jinshui district, Zhengzhou, Henan, 450003, ChinaOrthotopic rat liver transplantation (OLT) is a complex microsurgical procedure extensively applied to basic science, myriad complications can occur, but incision-related self-biting has not been reported after OLT. For the project of tolerance induction through stem cells, we performed OLT from Lewis to Brown Norway (BN) rats as an acute rejection model and divided the study was into the transverse incision group (n = 15) and midline incision group (n = 22), while cyclosporine A was subcutaneously injected for 10-day immunosuppression use, lidocaine cream was used for pain-relieving. The recipient survival and wound status were the primary endpoint of this study. For the transverse incision group, 30-day survival rate was 40% (6/15), self-biting occurred in 13 cases in 7–39 days, the degree 1 of biting occurred in 1 cases, the degree 2 in 2 cases. The degree 3 in 10 cases, which caused death or euthanasia, the self-biting rate was 86.7% (13/15), For the midline incision group, 30-day survival rate was 100% (22/22), the degree 1 of self-biting occurred in 3 cases, no severe self-biting occurred. There were significant differences for survival (p = 0.0003) and for self-biting rate (p < 0.01) between two groups. In conclusion, incision-related self-biting behavior occurs due to incisional injury, the transverse incision is severely pain-causing; the midline one is effective to avert occurrences.http://www.sciencedirect.com/science/article/pii/S240584402305421XOrthotopic liver transplantationRatSelf-biting |
| spellingShingle | Gaofeng Tang Huibo Zhao Guoyong Chen Shaotang Zhou Which incision is better for Lewis to Brown Norway rat liver transplantation, transverse or midline? Heliyon Orthotopic liver transplantation Rat Self-biting |
| title | Which incision is better for Lewis to Brown Norway rat liver transplantation, transverse or midline? |
| title_full | Which incision is better for Lewis to Brown Norway rat liver transplantation, transverse or midline? |
| title_fullStr | Which incision is better for Lewis to Brown Norway rat liver transplantation, transverse or midline? |
| title_full_unstemmed | Which incision is better for Lewis to Brown Norway rat liver transplantation, transverse or midline? |
| title_short | Which incision is better for Lewis to Brown Norway rat liver transplantation, transverse or midline? |
| title_sort | which incision is better for lewis to brown norway rat liver transplantation transverse or midline |
| topic | Orthotopic liver transplantation Rat Self-biting |
| url | http://www.sciencedirect.com/science/article/pii/S240584402305421X |
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