The silencing of Pokemon attenuates the proliferation of hepatocellular carcinoma cells in vitro and in vivo by inhibiting the PI3K/Akt pathway.
Pokemon (POK erythroid myeloid ontogenic factor), which belongs to the POK protein family, is also called LRF, OCZF and FBI-1. As a transcriptional repressor, Pokemon assumes a critical function in cellular differentiation and oncogenesis. Our study identified an oncogenic role for Pokemon in human...
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3530584?pdf=render |
| _version_ | 1811338286430420992 |
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| author | Chan-Chan Lin Jing-Ping Zhou Yun-Peng Liu Jing-Jing Liu Xiao-Ning Yang Amarsanaa Jazag Zhi-Ping Zhang Bayasi Guleng Jian-Lin Ren |
| author_facet | Chan-Chan Lin Jing-Ping Zhou Yun-Peng Liu Jing-Jing Liu Xiao-Ning Yang Amarsanaa Jazag Zhi-Ping Zhang Bayasi Guleng Jian-Lin Ren |
| author_sort | Chan-Chan Lin |
| collection | DOAJ |
| description | Pokemon (POK erythroid myeloid ontogenic factor), which belongs to the POK protein family, is also called LRF, OCZF and FBI-1. As a transcriptional repressor, Pokemon assumes a critical function in cellular differentiation and oncogenesis. Our study identified an oncogenic role for Pokemon in human hepatocellular carcinoma (HCC). We successfully established human HepG2 and Huh-7 cell lines in which Pokemon was stably knocked down. We demonstrated that Pokemon silencing inhibited cell proliferation and migration. Pokemon knockdown inhibited the PI3K/Akt and c-Raf/MEK/ERK pathways and modulated the expression of various cell cycle regulators in HepG2 and Huh-7 cells. Therefore, Pokemon may also be involved in cell cycle progression in these cells. We confirmed that Pokemon silencing suppresses hepatocellular carcinoma growth in tumor xenograft mice. These results suggest that Pokemon promotes cell proliferation and migration in hepatocellular carcinoma and accelerates tumor development in an Akt- and ERK-signaling-dependent manner. |
| first_indexed | 2024-04-13T18:07:40Z |
| format | Article |
| id | doaj.art-d23b9b066b854536ac3bb4b15bc6a495 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T18:07:40Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d23b9b066b854536ac3bb4b15bc6a4952022-12-22T02:36:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5191610.1371/journal.pone.0051916The silencing of Pokemon attenuates the proliferation of hepatocellular carcinoma cells in vitro and in vivo by inhibiting the PI3K/Akt pathway.Chan-Chan LinJing-Ping ZhouYun-Peng LiuJing-Jing LiuXiao-Ning YangAmarsanaa JazagZhi-Ping ZhangBayasi GulengJian-Lin RenPokemon (POK erythroid myeloid ontogenic factor), which belongs to the POK protein family, is also called LRF, OCZF and FBI-1. As a transcriptional repressor, Pokemon assumes a critical function in cellular differentiation and oncogenesis. Our study identified an oncogenic role for Pokemon in human hepatocellular carcinoma (HCC). We successfully established human HepG2 and Huh-7 cell lines in which Pokemon was stably knocked down. We demonstrated that Pokemon silencing inhibited cell proliferation and migration. Pokemon knockdown inhibited the PI3K/Akt and c-Raf/MEK/ERK pathways and modulated the expression of various cell cycle regulators in HepG2 and Huh-7 cells. Therefore, Pokemon may also be involved in cell cycle progression in these cells. We confirmed that Pokemon silencing suppresses hepatocellular carcinoma growth in tumor xenograft mice. These results suggest that Pokemon promotes cell proliferation and migration in hepatocellular carcinoma and accelerates tumor development in an Akt- and ERK-signaling-dependent manner.http://europepmc.org/articles/PMC3530584?pdf=render |
| spellingShingle | Chan-Chan Lin Jing-Ping Zhou Yun-Peng Liu Jing-Jing Liu Xiao-Ning Yang Amarsanaa Jazag Zhi-Ping Zhang Bayasi Guleng Jian-Lin Ren The silencing of Pokemon attenuates the proliferation of hepatocellular carcinoma cells in vitro and in vivo by inhibiting the PI3K/Akt pathway. PLoS ONE |
| title | The silencing of Pokemon attenuates the proliferation of hepatocellular carcinoma cells in vitro and in vivo by inhibiting the PI3K/Akt pathway. |
| title_full | The silencing of Pokemon attenuates the proliferation of hepatocellular carcinoma cells in vitro and in vivo by inhibiting the PI3K/Akt pathway. |
| title_fullStr | The silencing of Pokemon attenuates the proliferation of hepatocellular carcinoma cells in vitro and in vivo by inhibiting the PI3K/Akt pathway. |
| title_full_unstemmed | The silencing of Pokemon attenuates the proliferation of hepatocellular carcinoma cells in vitro and in vivo by inhibiting the PI3K/Akt pathway. |
| title_short | The silencing of Pokemon attenuates the proliferation of hepatocellular carcinoma cells in vitro and in vivo by inhibiting the PI3K/Akt pathway. |
| title_sort | silencing of pokemon attenuates the proliferation of hepatocellular carcinoma cells in vitro and in vivo by inhibiting the pi3k akt pathway |
| url | http://europepmc.org/articles/PMC3530584?pdf=render |
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