The Endothelial Mechanotransduction Protein Platelet Endothelial Cell Adhesion Molecule-1 Is Influenced by Aging and Exercise Training in Human Skeletal Muscle
Aim: The aim was to determine the role of aging and exercise training on endothelial mechanosensor proteins and the hyperemic response to shear stress by passive leg movement.Methods: We examined the expression of mechanosensor proteins and vascular function in young (n = 14, 25 ± 3 years) and old (...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-12-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2018.01807/full |
| _version_ | 1819157156420648960 |
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| author | Lasse Gliemann Nicolai Rytter Peter Piil Jannik Nilton Thomas Lind Michael Nyberg Matthew Cocks Ylva Hellsten |
| author_facet | Lasse Gliemann Nicolai Rytter Peter Piil Jannik Nilton Thomas Lind Michael Nyberg Matthew Cocks Ylva Hellsten |
| author_sort | Lasse Gliemann |
| collection | DOAJ |
| description | Aim: The aim was to determine the role of aging and exercise training on endothelial mechanosensor proteins and the hyperemic response to shear stress by passive leg movement.Methods: We examined the expression of mechanosensor proteins and vascular function in young (n = 14, 25 ± 3 years) and old (n = 14, 72 ± 5 years) healthy male subjects with eight weeks of aerobic exercise training. Before and after training, the hyperaemic response to passive leg movement was determined and a thigh muscle biopsy was obtained before and after passive leg movement to assess the acute effect of increased shear stress. Biopsies were analyzed for protein amount and phosphorylation of mechanosensor proteins; Platelet endothelial cell adhesion molecule-1 (PECAM-1), Vascular endothelial cadherin, Vascular endothelial growth factor receptor-2 and endothelial nitric oxide synthase (eNOS).Results: Before training, the old group presented a lower hyperaemic response to passive leg movement and a 35% lower (P < 0.05) relative basal phosphorylation level of PECAM-1 whereas there was no difference for the other mechanosensor proteins. After training, the eNOS protein amount, the amount of PECAM-1 protein and the passive leg movement-induced phosphorylation of PECAM-1 were higher in both groups. The hyperaemic response to passive leg movement was higher after training in the young group only.Conclusion: Aged individuals have a lower hyperaemic response to passive leg movement and a lower relative basal phosphorylation of PECAM-1 than young. The higher PECAM-1 phosphorylation despite a similar hyperemic level in the aged observed after training, suggests that training improved shear stress responsiveness of this mechanotransduction protein. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-22T16:04:17Z |
| publishDate | 2018-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-d23bcf5252834351a904390bd75fd9022022-12-21T18:20:38ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-12-01910.3389/fphys.2018.01807423599The Endothelial Mechanotransduction Protein Platelet Endothelial Cell Adhesion Molecule-1 Is Influenced by Aging and Exercise Training in Human Skeletal MuscleLasse Gliemann0Nicolai Rytter1Peter Piil2Jannik Nilton3Thomas Lind4Michael Nyberg5Matthew Cocks6Ylva Hellsten7Department of Nutrition, Exercise and Sports, Section for Integrative Physiology, University of Copenhagen, Copenhagen, DenmarkDepartment of Nutrition, Exercise and Sports, Section for Integrative Physiology, University of Copenhagen, Copenhagen, DenmarkDepartment of Nutrition, Exercise and Sports, Section for Integrative Physiology, University of Copenhagen, Copenhagen, DenmarkDepartment of Nutrition, Exercise and Sports, Section for Integrative Physiology, University of Copenhagen, Copenhagen, DenmarkDepartment of Nutrition, Exercise and Sports, Section for Integrative Physiology, University of Copenhagen, Copenhagen, DenmarkDepartment of Nutrition, Exercise and Sports, Section for Integrative Physiology, University of Copenhagen, Copenhagen, DenmarkExercise Metabolism Research Group, School of Sport and Exercise Sciences, University of Birmingham, Birmingham, United KingdomDepartment of Nutrition, Exercise and Sports, Section for Integrative Physiology, University of Copenhagen, Copenhagen, DenmarkAim: The aim was to determine the role of aging and exercise training on endothelial mechanosensor proteins and the hyperemic response to shear stress by passive leg movement.Methods: We examined the expression of mechanosensor proteins and vascular function in young (n = 14, 25 ± 3 years) and old (n = 14, 72 ± 5 years) healthy male subjects with eight weeks of aerobic exercise training. Before and after training, the hyperaemic response to passive leg movement was determined and a thigh muscle biopsy was obtained before and after passive leg movement to assess the acute effect of increased shear stress. Biopsies were analyzed for protein amount and phosphorylation of mechanosensor proteins; Platelet endothelial cell adhesion molecule-1 (PECAM-1), Vascular endothelial cadherin, Vascular endothelial growth factor receptor-2 and endothelial nitric oxide synthase (eNOS).Results: Before training, the old group presented a lower hyperaemic response to passive leg movement and a 35% lower (P < 0.05) relative basal phosphorylation level of PECAM-1 whereas there was no difference for the other mechanosensor proteins. After training, the eNOS protein amount, the amount of PECAM-1 protein and the passive leg movement-induced phosphorylation of PECAM-1 were higher in both groups. The hyperaemic response to passive leg movement was higher after training in the young group only.Conclusion: Aged individuals have a lower hyperaemic response to passive leg movement and a lower relative basal phosphorylation of PECAM-1 than young. The higher PECAM-1 phosphorylation despite a similar hyperemic level in the aged observed after training, suggests that training improved shear stress responsiveness of this mechanotransduction protein.https://www.frontiersin.org/article/10.3389/fphys.2018.01807/fullvascular functionpassive leg movementmechanosensorsaging – old age – seniorsshear stress |
| spellingShingle | Lasse Gliemann Nicolai Rytter Peter Piil Jannik Nilton Thomas Lind Michael Nyberg Matthew Cocks Ylva Hellsten The Endothelial Mechanotransduction Protein Platelet Endothelial Cell Adhesion Molecule-1 Is Influenced by Aging and Exercise Training in Human Skeletal Muscle Frontiers in Physiology vascular function passive leg movement mechanosensors aging – old age – seniors shear stress |
| title | The Endothelial Mechanotransduction Protein Platelet Endothelial Cell Adhesion Molecule-1 Is Influenced by Aging and Exercise Training in Human Skeletal Muscle |
| title_full | The Endothelial Mechanotransduction Protein Platelet Endothelial Cell Adhesion Molecule-1 Is Influenced by Aging and Exercise Training in Human Skeletal Muscle |
| title_fullStr | The Endothelial Mechanotransduction Protein Platelet Endothelial Cell Adhesion Molecule-1 Is Influenced by Aging and Exercise Training in Human Skeletal Muscle |
| title_full_unstemmed | The Endothelial Mechanotransduction Protein Platelet Endothelial Cell Adhesion Molecule-1 Is Influenced by Aging and Exercise Training in Human Skeletal Muscle |
| title_short | The Endothelial Mechanotransduction Protein Platelet Endothelial Cell Adhesion Molecule-1 Is Influenced by Aging and Exercise Training in Human Skeletal Muscle |
| title_sort | endothelial mechanotransduction protein platelet endothelial cell adhesion molecule 1 is influenced by aging and exercise training in human skeletal muscle |
| topic | vascular function passive leg movement mechanosensors aging – old age – seniors shear stress |
| url | https://www.frontiersin.org/article/10.3389/fphys.2018.01807/full |
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