miRNA profiling of chicken follicles during follicular development
Abstract MicroRNAs (miRNAs) play a crucial role as transcription regulators in various aspects of follicular development, including steroidogenesis, ovulation, apoptosis, and gene regulation in poultry. However, there is a paucity of studies examining the specific impact of miRNAs on ovarian granulo...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-52716-x |
| _version_ | 1797276639127666688 |
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| author | Zhongxian Xu Qian Liu Chunyou Ning Maosen Yang Qing Zhu Diyan Li Tao Wang Feng Li |
| author_facet | Zhongxian Xu Qian Liu Chunyou Ning Maosen Yang Qing Zhu Diyan Li Tao Wang Feng Li |
| author_sort | Zhongxian Xu |
| collection | DOAJ |
| description | Abstract MicroRNAs (miRNAs) play a crucial role as transcription regulators in various aspects of follicular development, including steroidogenesis, ovulation, apoptosis, and gene regulation in poultry. However, there is a paucity of studies examining the specific impact of miRNAs on ovarian granulosa cells (GCs) across multiple grades in laying hens. Consequently, this study aims to investigate the roles of miRNAs in chicken GCs. By constructing miRNA expression profiles of GCs at 10 different time points, encompassing 4 pre-hierarchical, 5 preovulatory, and 1 postovulatory follicles stage, we identified highly expressed miRNAs involved in GC differentiation (miR-148a-3p, miR-143-3p), apoptosis (let7 family, miR-363-3p, miR-30c-5p, etc.), and autophagy (miR-128-3p, miR-21-5p). Furthermore, we discovered 48 developmentally dynamic miRNAs (DDMs) that target 295 dynamic differentially expressed genes (DDGs) associated with follicular development and selection (such as oocyte meiosis, progesterone-mediated oocyte maturation, Wnt signaling pathway, TGF-β signaling pathway) as well as follicular regression (including autophagy and cellular senescence). These findings contribute to a more comprehensive understanding of the intricate mechanisms underlying follicle recruitment, selection, and degeneration, aiming to enhance poultry’s reproductive capacity. |
| first_indexed | 2024-03-07T15:31:07Z |
| format | Article |
| id | doaj.art-d23da8b88aa04675bf19a5bec61190a6 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:31:07Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d23da8b88aa04675bf19a5bec61190a62024-03-05T16:25:33ZengNature PortfolioScientific Reports2045-23222024-01-0114111310.1038/s41598-024-52716-xmiRNA profiling of chicken follicles during follicular developmentZhongxian Xu0Qian Liu1Chunyou Ning2Maosen Yang3Qing Zhu4Diyan Li5Tao Wang6Feng Li7Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal UniversityKey Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal UniversityInstitute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural UniversityInstitute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural UniversityInstitute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural UniversityAntibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, School of Pharmacy, Chengdu UniversityAntibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, School of Pharmacy, Chengdu UniversityKey Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal UniversityAbstract MicroRNAs (miRNAs) play a crucial role as transcription regulators in various aspects of follicular development, including steroidogenesis, ovulation, apoptosis, and gene regulation in poultry. However, there is a paucity of studies examining the specific impact of miRNAs on ovarian granulosa cells (GCs) across multiple grades in laying hens. Consequently, this study aims to investigate the roles of miRNAs in chicken GCs. By constructing miRNA expression profiles of GCs at 10 different time points, encompassing 4 pre-hierarchical, 5 preovulatory, and 1 postovulatory follicles stage, we identified highly expressed miRNAs involved in GC differentiation (miR-148a-3p, miR-143-3p), apoptosis (let7 family, miR-363-3p, miR-30c-5p, etc.), and autophagy (miR-128-3p, miR-21-5p). Furthermore, we discovered 48 developmentally dynamic miRNAs (DDMs) that target 295 dynamic differentially expressed genes (DDGs) associated with follicular development and selection (such as oocyte meiosis, progesterone-mediated oocyte maturation, Wnt signaling pathway, TGF-β signaling pathway) as well as follicular regression (including autophagy and cellular senescence). These findings contribute to a more comprehensive understanding of the intricate mechanisms underlying follicle recruitment, selection, and degeneration, aiming to enhance poultry’s reproductive capacity.https://doi.org/10.1038/s41598-024-52716-x |
| spellingShingle | Zhongxian Xu Qian Liu Chunyou Ning Maosen Yang Qing Zhu Diyan Li Tao Wang Feng Li miRNA profiling of chicken follicles during follicular development Scientific Reports |
| title | miRNA profiling of chicken follicles during follicular development |
| title_full | miRNA profiling of chicken follicles during follicular development |
| title_fullStr | miRNA profiling of chicken follicles during follicular development |
| title_full_unstemmed | miRNA profiling of chicken follicles during follicular development |
| title_short | miRNA profiling of chicken follicles during follicular development |
| title_sort | mirna profiling of chicken follicles during follicular development |
| url | https://doi.org/10.1038/s41598-024-52716-x |
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