Unveiling the Mutations and Conservation of InlA in <i>Listeria monocytogenes</i>
<i>Listeria monocytogenes</i> (<i>L. monocytogenes</i>) is a pathogen that is transmitted through contaminated food and causes the illness known as listeriosis. The virulence factor InlA plays a crucial role in the invasion of <i>L. monocytogenes</i> into the huma...
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MDPI AG
2024-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/12/3/485 |
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| author | Lingling Li Yan Wang Ji Pu Jinni Chen Lingyun Liu Pan Mao Hui Sun Xia Luo Changyun Ye |
| author_facet | Lingling Li Yan Wang Ji Pu Jinni Chen Lingyun Liu Pan Mao Hui Sun Xia Luo Changyun Ye |
| author_sort | Lingling Li |
| collection | DOAJ |
| description | <i>Listeria monocytogenes</i> (<i>L. monocytogenes</i>) is a pathogen that is transmitted through contaminated food and causes the illness known as listeriosis. The virulence factor InlA plays a crucial role in the invasion of <i>L. monocytogenes</i> into the human intestinal epithelium. In addition, InlA enhances the pathogenicity of host strains, and different strains of <i>L. monocytogenes</i> contain varying variations of InlA. Our study analyzed a total of 4393 published <i>L. monocytogenes</i> genomes from 511 sequence types (STs) of diverse origins. We identified 300 unique InlA protein sequence types (PSTs) and revealed 45 highly mutated amino acid sites. The leucine-rich repeat (LRR) region was found to be the most conserved among the InlA, while the protein A (PA) region experienced the highest mutation rate. Two new types of mutations were identified in the B-repeat region of InlA. Correspondence analysis (CA) was used to analyze correlations between the lineages or 10 most common sequence types (STs) and amino acid (aa) sites. ST8 was strongly correlated with site 192_F, 454_T. ST7 exhibited a strong correlation with site 51_A, 573_E, 648_S, and 664_A, and it was also associated with ST6 and site 544_N, 671_A, 738_B, 739_B, 740_B, and 774_Y. Additionally, a strong correlation between ST1 and site 142_S, 738_N, ST2 and site 2_K, 142_S, 738_N, as well as ST87 and site2_K, 738_N was demonstrated. Our findings contribute significantly to the understanding of the distribution, composition, and conservation of InlA in <i>L. monocytogenes.</i> These findings also suggest a potential role of InlA in supporting molecular epidemiological tracing efforts. |
| first_indexed | 2024-04-24T18:00:53Z |
| format | Article |
| id | doaj.art-d23f2d21afbd418fbbdb4cd4884753e5 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-04-24T18:00:53Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-d23f2d21afbd418fbbdb4cd4884753e52024-03-27T13:55:36ZengMDPI AGMicroorganisms2076-26072024-02-0112348510.3390/microorganisms12030485Unveiling the Mutations and Conservation of InlA in <i>Listeria monocytogenes</i>Lingling Li0Yan Wang1Ji Pu2Jinni Chen3Lingyun Liu4Pan Mao5Hui Sun6Xia Luo7Changyun Ye8National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China<i>Listeria monocytogenes</i> (<i>L. monocytogenes</i>) is a pathogen that is transmitted through contaminated food and causes the illness known as listeriosis. The virulence factor InlA plays a crucial role in the invasion of <i>L. monocytogenes</i> into the human intestinal epithelium. In addition, InlA enhances the pathogenicity of host strains, and different strains of <i>L. monocytogenes</i> contain varying variations of InlA. Our study analyzed a total of 4393 published <i>L. monocytogenes</i> genomes from 511 sequence types (STs) of diverse origins. We identified 300 unique InlA protein sequence types (PSTs) and revealed 45 highly mutated amino acid sites. The leucine-rich repeat (LRR) region was found to be the most conserved among the InlA, while the protein A (PA) region experienced the highest mutation rate. Two new types of mutations were identified in the B-repeat region of InlA. Correspondence analysis (CA) was used to analyze correlations between the lineages or 10 most common sequence types (STs) and amino acid (aa) sites. ST8 was strongly correlated with site 192_F, 454_T. ST7 exhibited a strong correlation with site 51_A, 573_E, 648_S, and 664_A, and it was also associated with ST6 and site 544_N, 671_A, 738_B, 739_B, 740_B, and 774_Y. Additionally, a strong correlation between ST1 and site 142_S, 738_N, ST2 and site 2_K, 142_S, 738_N, as well as ST87 and site2_K, 738_N was demonstrated. Our findings contribute significantly to the understanding of the distribution, composition, and conservation of InlA in <i>L. monocytogenes.</i> These findings also suggest a potential role of InlA in supporting molecular epidemiological tracing efforts.https://www.mdpi.com/2076-2607/12/3/485<i>Listeria monocytogenes</i>InlASTamino acidprotein sequence type |
| spellingShingle | Lingling Li Yan Wang Ji Pu Jinni Chen Lingyun Liu Pan Mao Hui Sun Xia Luo Changyun Ye Unveiling the Mutations and Conservation of InlA in <i>Listeria monocytogenes</i> Microorganisms <i>Listeria monocytogenes</i> InlA ST amino acid protein sequence type |
| title | Unveiling the Mutations and Conservation of InlA in <i>Listeria monocytogenes</i> |
| title_full | Unveiling the Mutations and Conservation of InlA in <i>Listeria monocytogenes</i> |
| title_fullStr | Unveiling the Mutations and Conservation of InlA in <i>Listeria monocytogenes</i> |
| title_full_unstemmed | Unveiling the Mutations and Conservation of InlA in <i>Listeria monocytogenes</i> |
| title_short | Unveiling the Mutations and Conservation of InlA in <i>Listeria monocytogenes</i> |
| title_sort | unveiling the mutations and conservation of inla in i listeria monocytogenes i |
| topic | <i>Listeria monocytogenes</i> InlA ST amino acid protein sequence type |
| url | https://www.mdpi.com/2076-2607/12/3/485 |
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