A Subpopulation of Label-Retaining Cells of the Kidney Papilla Regenerates Injured Kidney Medullary Tubules
To determine whether adult kidney papillary label-retaining cells (pLRCs) are specialized precursors, we analyzed their transcription profile. Among genes overexpressed in pLRCs, we selected candidate genes to perform qPCR and immunodetection of their encoded proteins. We found that Zfyve27, which e...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-05-01
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| Series: | Stem Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671116300030 |
| _version_ | 1818693036112084992 |
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| author | Juan A. Oliver Rosemary V. Sampogna Sumreen Jalal Qing-Yin Zhang Alexander Dahan Weiwei Wang Tian Huai Shen Qais Al-Awqati |
| author_facet | Juan A. Oliver Rosemary V. Sampogna Sumreen Jalal Qing-Yin Zhang Alexander Dahan Weiwei Wang Tian Huai Shen Qais Al-Awqati |
| author_sort | Juan A. Oliver |
| collection | DOAJ |
| description | To determine whether adult kidney papillary label-retaining cells (pLRCs) are specialized precursors, we analyzed their transcription profile. Among genes overexpressed in pLRCs, we selected candidate genes to perform qPCR and immunodetection of their encoded proteins. We found that Zfyve27, which encodes protrudin, identified a subpopulation of pLRCs. With Zfyve27-CreERT2 transgenic and reporter mice we generated bitransgenic animals and performed cell-lineage analysis. Post tamoxifen, Zfyve27-CreERT2 marked cells preferentially located in the upper part of the papilla. These cells were low cycling and did not generate progeny even after long-term observation, thus they did not appear to contribute to kidney homeostasis. However, after kidney injury, but only if severe, they activated a program of proliferation, migration, and morphogenesis generating multiple and long tubular segments. Remarkably these regenerated tubules were located preferentially in the kidney medulla, indicating that repair of injury in the kidney is regionally specified. These results suggest that different parts of the kidney have different progenitor cell pools. |
| first_indexed | 2024-12-17T13:07:17Z |
| format | Article |
| id | doaj.art-d24146ef19bb43e9bfc701279801ddb6 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-12-17T13:07:17Z |
| publishDate | 2016-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-d24146ef19bb43e9bfc701279801ddb62022-12-21T21:47:13ZengElsevierStem Cell Reports2213-67112016-05-016575777110.1016/j.stemcr.2016.03.008A Subpopulation of Label-Retaining Cells of the Kidney Papilla Regenerates Injured Kidney Medullary TubulesJuan A. Oliver0Rosemary V. Sampogna1Sumreen Jalal2Qing-Yin Zhang3Alexander Dahan4Weiwei Wang5Tian Huai Shen6Qais Al-Awqati7Department of Medicine, Columbia University, New York, NY 10032, USADepartment of Medicine, Columbia University, New York, NY 10032, USADepartment of Medicine, Columbia University, New York, NY 10032, USADepartment of Pathology and Cell Biology, Columbia University, New York, NY 10032, USADepartment of Medicine, Columbia University, New York, NY 10032, USADepartment of Medicine, Columbia University, New York, NY 10032, USADepartment of Medicine, Columbia University, New York, NY 10032, USADepartment of Medicine, Columbia University, New York, NY 10032, USATo determine whether adult kidney papillary label-retaining cells (pLRCs) are specialized precursors, we analyzed their transcription profile. Among genes overexpressed in pLRCs, we selected candidate genes to perform qPCR and immunodetection of their encoded proteins. We found that Zfyve27, which encodes protrudin, identified a subpopulation of pLRCs. With Zfyve27-CreERT2 transgenic and reporter mice we generated bitransgenic animals and performed cell-lineage analysis. Post tamoxifen, Zfyve27-CreERT2 marked cells preferentially located in the upper part of the papilla. These cells were low cycling and did not generate progeny even after long-term observation, thus they did not appear to contribute to kidney homeostasis. However, after kidney injury, but only if severe, they activated a program of proliferation, migration, and morphogenesis generating multiple and long tubular segments. Remarkably these regenerated tubules were located preferentially in the kidney medulla, indicating that repair of injury in the kidney is regionally specified. These results suggest that different parts of the kidney have different progenitor cell pools.http://www.sciencedirect.com/science/article/pii/S2213671116300030 |
| spellingShingle | Juan A. Oliver Rosemary V. Sampogna Sumreen Jalal Qing-Yin Zhang Alexander Dahan Weiwei Wang Tian Huai Shen Qais Al-Awqati A Subpopulation of Label-Retaining Cells of the Kidney Papilla Regenerates Injured Kidney Medullary Tubules Stem Cell Reports |
| title | A Subpopulation of Label-Retaining Cells of the Kidney Papilla Regenerates Injured Kidney Medullary Tubules |
| title_full | A Subpopulation of Label-Retaining Cells of the Kidney Papilla Regenerates Injured Kidney Medullary Tubules |
| title_fullStr | A Subpopulation of Label-Retaining Cells of the Kidney Papilla Regenerates Injured Kidney Medullary Tubules |
| title_full_unstemmed | A Subpopulation of Label-Retaining Cells of the Kidney Papilla Regenerates Injured Kidney Medullary Tubules |
| title_short | A Subpopulation of Label-Retaining Cells of the Kidney Papilla Regenerates Injured Kidney Medullary Tubules |
| title_sort | subpopulation of label retaining cells of the kidney papilla regenerates injured kidney medullary tubules |
| url | http://www.sciencedirect.com/science/article/pii/S2213671116300030 |
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