Growth Factors Do Not Improve Muscle Function in Young or Adult <i>mdx</i> Mice
Muscular dystrophies constitute a broad group of genetic disorders leading to muscle wasting. We have previously demonstrated that treating a muscular atrophy mouse model with growth factors resulted in increased muscle mass. In the present study, we treated the Duchenne mouse model <i>mdx<...
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MDPI AG
2022-01-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/2/304 |
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| author | Tue L. Nielsen Tessa M. Hornsyld Tomàs Pinós Camilla Brolin John Vissing Thomas O. Krag |
| author_facet | Tue L. Nielsen Tessa M. Hornsyld Tomàs Pinós Camilla Brolin John Vissing Thomas O. Krag |
| author_sort | Tue L. Nielsen |
| collection | DOAJ |
| description | Muscular dystrophies constitute a broad group of genetic disorders leading to muscle wasting. We have previously demonstrated that treating a muscular atrophy mouse model with growth factors resulted in increased muscle mass. In the present study, we treated the Duchenne mouse model <i>mdx</i> for 12 weeks with myogenic growth factors peri- and post-onset of muscular degeneration to explore the effects in the oxidative muscle soleus and the glycolytic muscle extensor digitorum longus (EDL). We found no overall beneficial effect in the peri-onset group at the conclusion of the study. In the post-onset group, the functional improvement by means of electrophysiological examinations ex vivo was mostly confined to the soleus. EDL benefitted from the treatment on a molecular level but did not improve functionally. Histopathology revealed signs of inflammation at the end of treatment. In conclusion, the growth factor cocktail failed to improve the <i>mdx</i> on a functional level. |
| first_indexed | 2024-03-09T22:33:15Z |
| format | Article |
| id | doaj.art-d241c62871344fea952fa012983a99f4 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T22:33:15Z |
| publishDate | 2022-01-01 |
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| spelling | doaj.art-d241c62871344fea952fa012983a99f42023-11-23T18:53:32ZengMDPI AGBiomedicines2227-90592022-01-0110230410.3390/biomedicines10020304Growth Factors Do Not Improve Muscle Function in Young or Adult <i>mdx</i> MiceTue L. Nielsen0Tessa M. Hornsyld1Tomàs Pinós2Camilla Brolin3John Vissing4Thomas O. Krag5Copenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen, DenmarkCopenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen, DenmarkMitochondrial and Neuromuscular Disorders Unit, Vall d’Hebron Institut de Recerca, Universitat Autònoma de Barcelona, 08035 Barcelona, SpainDepartment of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, The Panum Institute, University of Copenhagen, DK-2200 Copenhagen, DenmarkCopenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen, DenmarkCopenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen, DenmarkMuscular dystrophies constitute a broad group of genetic disorders leading to muscle wasting. We have previously demonstrated that treating a muscular atrophy mouse model with growth factors resulted in increased muscle mass. In the present study, we treated the Duchenne mouse model <i>mdx</i> for 12 weeks with myogenic growth factors peri- and post-onset of muscular degeneration to explore the effects in the oxidative muscle soleus and the glycolytic muscle extensor digitorum longus (EDL). We found no overall beneficial effect in the peri-onset group at the conclusion of the study. In the post-onset group, the functional improvement by means of electrophysiological examinations ex vivo was mostly confined to the soleus. EDL benefitted from the treatment on a molecular level but did not improve functionally. Histopathology revealed signs of inflammation at the end of treatment. In conclusion, the growth factor cocktail failed to improve the <i>mdx</i> on a functional level.https://www.mdpi.com/2227-9059/10/2/304<i>mdx</i>DMDmuscular regenerationhepatocyte growth factorleukemia inhibitory factor |
| spellingShingle | Tue L. Nielsen Tessa M. Hornsyld Tomàs Pinós Camilla Brolin John Vissing Thomas O. Krag Growth Factors Do Not Improve Muscle Function in Young or Adult <i>mdx</i> Mice Biomedicines <i>mdx</i> DMD muscular regeneration hepatocyte growth factor leukemia inhibitory factor |
| title | Growth Factors Do Not Improve Muscle Function in Young or Adult <i>mdx</i> Mice |
| title_full | Growth Factors Do Not Improve Muscle Function in Young or Adult <i>mdx</i> Mice |
| title_fullStr | Growth Factors Do Not Improve Muscle Function in Young or Adult <i>mdx</i> Mice |
| title_full_unstemmed | Growth Factors Do Not Improve Muscle Function in Young or Adult <i>mdx</i> Mice |
| title_short | Growth Factors Do Not Improve Muscle Function in Young or Adult <i>mdx</i> Mice |
| title_sort | growth factors do not improve muscle function in young or adult i mdx i mice |
| topic | <i>mdx</i> DMD muscular regeneration hepatocyte growth factor leukemia inhibitory factor |
| url | https://www.mdpi.com/2227-9059/10/2/304 |
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