Targetome Analysis Revealed Involvement of MiR-126 in Neurotrophin Signaling Pathway: A Possible Role in Prevention of Glioma Development
Objective: For the first time, we used molecular signaling pathway enrichment analysis to determine possible involvement of miR-126 and IRS-1 in neurotrophin pathway. Materials and Methods: In this prospective study, validated and predicted targets (targetome) of miR-126 were collected following...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2018-04-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/journal/article/19658/download |
| Summary: | Objective: For the first time, we used molecular signaling pathway enrichment analysis to determine possible involvement of
miR-126 and IRS-1 in neurotrophin pathway.
Materials and Methods: In this prospective study, validated and predicted targets (targetome) of miR-126 were collected
following searching miRtarbase (http://mirtarbase.mbc.nctu.edu.tw/) and miRWalk 2.0 databases, respectively. Then,
approximate expression of miR-126 targeting in Glioma tissue was examined using UniGene database (http://www.ncbi.
nlm.nih.gov/unigene). In silico molecular pathway enrichment analysis was carried out by DAVID 6.7 database (http://david.
abcc.ncifcrf.gov/) to explore which signaling pathway is related to miR-126 targeting and how miR-126 attributes to glioma
development.
Results: MiR-126 exerts a variety of functions in cancer pathogenesis via suppression of expression of target gene
including PI3K, KRAS, EGFL7, IRS-1 and VEGF. Our bioinformatic studies implementing DAVID database, showed
the involvement of miR-126 target genes in several signaling pathways including cancer pathogenesis, neurotrophin
functions, Glioma formation, insulin function, focal adhesion production, chemokine synthesis and secretion and
regulation of the actin cytoskeleton.
Conclusion: Taken together, we concluded that miR-126 enhances the formation of glioma cancer stem cell probably
via down regulation of IRS-1 in neurotrophin signaling pathway. |
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| ISSN: | 2228-5806 2228-5814 |