Targetome Analysis Revealed Involvement of MiR-126 in Neurotrophin Signaling Pathway: A Possible Role in Prevention of Glioma Development
Objective: For the first time, we used molecular signaling pathway enrichment analysis to determine possible involvement of miR-126 and IRS-1 in neurotrophin pathway. Materials and Methods: In this prospective study, validated and predicted targets (targetome) of miR-126 were collected following...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2018-04-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/journal/article/19658/download |
| _version_ | 1818264199541817344 |
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| author | Maedeh Rouigari Moein Dehbashi Kamran Ghaedi Meraj Pourhossein |
| author_facet | Maedeh Rouigari Moein Dehbashi Kamran Ghaedi Meraj Pourhossein |
| author_sort | Maedeh Rouigari |
| collection | DOAJ |
| description | Objective: For the first time, we used molecular signaling pathway enrichment analysis to determine possible involvement of
miR-126 and IRS-1 in neurotrophin pathway.
Materials and Methods: In this prospective study, validated and predicted targets (targetome) of miR-126 were collected
following searching miRtarbase (http://mirtarbase.mbc.nctu.edu.tw/) and miRWalk 2.0 databases, respectively. Then,
approximate expression of miR-126 targeting in Glioma tissue was examined using UniGene database (http://www.ncbi.
nlm.nih.gov/unigene). In silico molecular pathway enrichment analysis was carried out by DAVID 6.7 database (http://david.
abcc.ncifcrf.gov/) to explore which signaling pathway is related to miR-126 targeting and how miR-126 attributes to glioma
development.
Results: MiR-126 exerts a variety of functions in cancer pathogenesis via suppression of expression of target gene
including PI3K, KRAS, EGFL7, IRS-1 and VEGF. Our bioinformatic studies implementing DAVID database, showed
the involvement of miR-126 target genes in several signaling pathways including cancer pathogenesis, neurotrophin
functions, Glioma formation, insulin function, focal adhesion production, chemokine synthesis and secretion and
regulation of the actin cytoskeleton.
Conclusion: Taken together, we concluded that miR-126 enhances the formation of glioma cancer stem cell probably
via down regulation of IRS-1 in neurotrophin signaling pathway. |
| first_indexed | 2024-12-12T19:31:07Z |
| format | Article |
| id | doaj.art-d2431b06a27a43abba1a46b1a6398991 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-12T19:31:07Z |
| publishDate | 2018-04-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-d2431b06a27a43abba1a46b1a63989912022-12-22T00:14:25ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142018-04-0120215015610.22074/cellj.2018.4901Targetome Analysis Revealed Involvement of MiR-126 in Neurotrophin Signaling Pathway: A Possible Role in Prevention of Glioma DevelopmentMaedeh Rouigari0Moein Dehbashi1Kamran Ghaedi2Meraj Pourhossein3Isfahan Neuroscience Research Center (INRC), Alzahra Hospital, Isfahan University of Medical Sciences, Isfahan, IranIsfahan Neuroscience Research Center (INRC), Alzahra Hospital, Isfahan University of Medical Sciences, Isfahan, IranCell and Molecular Biology Division, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, IranDepartment of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences Isfahan, IranObjective: For the first time, we used molecular signaling pathway enrichment analysis to determine possible involvement of miR-126 and IRS-1 in neurotrophin pathway. Materials and Methods: In this prospective study, validated and predicted targets (targetome) of miR-126 were collected following searching miRtarbase (http://mirtarbase.mbc.nctu.edu.tw/) and miRWalk 2.0 databases, respectively. Then, approximate expression of miR-126 targeting in Glioma tissue was examined using UniGene database (http://www.ncbi. nlm.nih.gov/unigene). In silico molecular pathway enrichment analysis was carried out by DAVID 6.7 database (http://david. abcc.ncifcrf.gov/) to explore which signaling pathway is related to miR-126 targeting and how miR-126 attributes to glioma development. Results: MiR-126 exerts a variety of functions in cancer pathogenesis via suppression of expression of target gene including PI3K, KRAS, EGFL7, IRS-1 and VEGF. Our bioinformatic studies implementing DAVID database, showed the involvement of miR-126 target genes in several signaling pathways including cancer pathogenesis, neurotrophin functions, Glioma formation, insulin function, focal adhesion production, chemokine synthesis and secretion and regulation of the actin cytoskeleton. Conclusion: Taken together, we concluded that miR-126 enhances the formation of glioma cancer stem cell probably via down regulation of IRS-1 in neurotrophin signaling pathway.http://celljournal.org/journal/article/19658/downloadEGFL7GliomaIRS-1MiR-126Neurotrophin |
| spellingShingle | Maedeh Rouigari Moein Dehbashi Kamran Ghaedi Meraj Pourhossein Targetome Analysis Revealed Involvement of MiR-126 in Neurotrophin Signaling Pathway: A Possible Role in Prevention of Glioma Development Cell Journal EGFL7 Glioma IRS-1 MiR-126 Neurotrophin |
| title | Targetome Analysis Revealed Involvement of MiR-126 in Neurotrophin Signaling Pathway: A Possible Role in Prevention of Glioma Development |
| title_full | Targetome Analysis Revealed Involvement of MiR-126 in Neurotrophin Signaling Pathway: A Possible Role in Prevention of Glioma Development |
| title_fullStr | Targetome Analysis Revealed Involvement of MiR-126 in Neurotrophin Signaling Pathway: A Possible Role in Prevention of Glioma Development |
| title_full_unstemmed | Targetome Analysis Revealed Involvement of MiR-126 in Neurotrophin Signaling Pathway: A Possible Role in Prevention of Glioma Development |
| title_short | Targetome Analysis Revealed Involvement of MiR-126 in Neurotrophin Signaling Pathway: A Possible Role in Prevention of Glioma Development |
| title_sort | targetome analysis revealed involvement of mir 126 in neurotrophin signaling pathway a possible role in prevention of glioma development |
| topic | EGFL7 Glioma IRS-1 MiR-126 Neurotrophin |
| url | http://celljournal.org/journal/article/19658/download |
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