Açaì (<i>Euterpe oleracea</i>) Extract Protects Human Erythrocytes from Age-Related Oxidative Stress
Aging is a process characterised by a general decline in physiological functions. The high bioavailability of reactive oxygen species (ROS) plays an important role in the aging rate. Due to the close relationship between aging and oxidative stress (OS), functional foods rich in flavonoids are excell...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/15/2391 |
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| author | Alessia Remigante Sara Spinelli Elisabetta Straface Lucrezia Gambardella Daniele Caruso Giuseppe Falliti Silvia Dossena Angela Marino Rossana Morabito |
| author_facet | Alessia Remigante Sara Spinelli Elisabetta Straface Lucrezia Gambardella Daniele Caruso Giuseppe Falliti Silvia Dossena Angela Marino Rossana Morabito |
| author_sort | Alessia Remigante |
| collection | DOAJ |
| description | Aging is a process characterised by a general decline in physiological functions. The high bioavailability of reactive oxygen species (ROS) plays an important role in the aging rate. Due to the close relationship between aging and oxidative stress (OS), functional foods rich in flavonoids are excellent candidates to counteract age-related changes. This study aimed to verify the protective role of Açaì extract in a <span style="font-variant: small-caps;">d</span>-Galactose (<span style="font-variant: small-caps;">d</span>-Gal)-induced model of aging in human erythrocytes. Markers of OS, including ROS production, thiobarbituric acid reactive substances (TBARS) levels, oxidation of protein sulfhydryl groups, as well as the anion exchange capability through Band 3 protein (B3p) and glycated haemoglobin (A1c) have been analysed in erythrocytes treated with <span style="font-variant: small-caps;">d</span>-Gal for 24 h, with or without pre-incubation for 1 h with 0.5–10 µg/mL Açaì extract. Our results show that the extract avoided the formation of acanthocytes and leptocytes observed after exposure to 50 and 100 mM <span style="font-variant: small-caps;">d</span>-Gal, respectively, prevented <span style="font-variant: small-caps;">d</span>-Gal-induced OS damage, and restored alterations in the distribution of B3p and CD47 proteins. Interestingly, <span style="font-variant: small-caps;">d</span>-Gal exposure was associated with an acceleration of the rate constant of SO<sub>4</sub><sup>2−</sup> uptake through B3p, as well as A1c formation. Both alterations have been attenuated by pre-treatment with the Açaì extract. These findings contribute to clarify the aging mechanisms in human erythrocytes and propose functional foods rich in flavonoids as natural antioxidants for the treatment and prevention of OS-related disease conditions. |
| first_indexed | 2024-03-09T10:09:53Z |
| format | Article |
| id | doaj.art-d244939df4184fa9bef0b3d18a708a20 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T10:09:53Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-d244939df4184fa9bef0b3d18a708a202023-12-01T22:53:02ZengMDPI AGCells2073-44092022-08-011115239110.3390/cells11152391Açaì (<i>Euterpe oleracea</i>) Extract Protects Human Erythrocytes from Age-Related Oxidative StressAlessia Remigante0Sara Spinelli1Elisabetta Straface2Lucrezia Gambardella3Daniele Caruso4Giuseppe Falliti5Silvia Dossena6Angela Marino7Rossana Morabito8Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, ItalyBiomarkers Unit, Center for Gender-Specific Medicine, Istituto Superiore di Sanità, 00161 Rome, ItalyBiomarkers Unit, Center for Gender-Specific Medicine, Istituto Superiore di Sanità, 00161 Rome, ItalyComplex Operational Unit of Clinical Pathology of Papardo Hospital, 98166 Messina, ItalyComplex Operational Unit of Clinical Pathology of Papardo Hospital, 98166 Messina, ItalyInstitute of Pharmacology and Toxicology, Paracelsus Medical University, 5020 Salzburg, AustriaDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, ItalyAging is a process characterised by a general decline in physiological functions. The high bioavailability of reactive oxygen species (ROS) plays an important role in the aging rate. Due to the close relationship between aging and oxidative stress (OS), functional foods rich in flavonoids are excellent candidates to counteract age-related changes. This study aimed to verify the protective role of Açaì extract in a <span style="font-variant: small-caps;">d</span>-Galactose (<span style="font-variant: small-caps;">d</span>-Gal)-induced model of aging in human erythrocytes. Markers of OS, including ROS production, thiobarbituric acid reactive substances (TBARS) levels, oxidation of protein sulfhydryl groups, as well as the anion exchange capability through Band 3 protein (B3p) and glycated haemoglobin (A1c) have been analysed in erythrocytes treated with <span style="font-variant: small-caps;">d</span>-Gal for 24 h, with or without pre-incubation for 1 h with 0.5–10 µg/mL Açaì extract. Our results show that the extract avoided the formation of acanthocytes and leptocytes observed after exposure to 50 and 100 mM <span style="font-variant: small-caps;">d</span>-Gal, respectively, prevented <span style="font-variant: small-caps;">d</span>-Gal-induced OS damage, and restored alterations in the distribution of B3p and CD47 proteins. Interestingly, <span style="font-variant: small-caps;">d</span>-Gal exposure was associated with an acceleration of the rate constant of SO<sub>4</sub><sup>2−</sup> uptake through B3p, as well as A1c formation. Both alterations have been attenuated by pre-treatment with the Açaì extract. These findings contribute to clarify the aging mechanisms in human erythrocytes and propose functional foods rich in flavonoids as natural antioxidants for the treatment and prevention of OS-related disease conditions.https://www.mdpi.com/2073-4409/11/15/2391Açaí berry<span style="font-variant: small-caps">d</span>-Galactoseagingoxidative stressglycationplasma membrane |
| spellingShingle | Alessia Remigante Sara Spinelli Elisabetta Straface Lucrezia Gambardella Daniele Caruso Giuseppe Falliti Silvia Dossena Angela Marino Rossana Morabito Açaì (<i>Euterpe oleracea</i>) Extract Protects Human Erythrocytes from Age-Related Oxidative Stress Cells Açaí berry <span style="font-variant: small-caps">d</span>-Galactose aging oxidative stress glycation plasma membrane |
| title | Açaì (<i>Euterpe oleracea</i>) Extract Protects Human Erythrocytes from Age-Related Oxidative Stress |
| title_full | Açaì (<i>Euterpe oleracea</i>) Extract Protects Human Erythrocytes from Age-Related Oxidative Stress |
| title_fullStr | Açaì (<i>Euterpe oleracea</i>) Extract Protects Human Erythrocytes from Age-Related Oxidative Stress |
| title_full_unstemmed | Açaì (<i>Euterpe oleracea</i>) Extract Protects Human Erythrocytes from Age-Related Oxidative Stress |
| title_short | Açaì (<i>Euterpe oleracea</i>) Extract Protects Human Erythrocytes from Age-Related Oxidative Stress |
| title_sort | acai i euterpe oleracea i extract protects human erythrocytes from age related oxidative stress |
| topic | Açaí berry <span style="font-variant: small-caps">d</span>-Galactose aging oxidative stress glycation plasma membrane |
| url | https://www.mdpi.com/2073-4409/11/15/2391 |
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