Effects of Tebuconazole Application on Soil Microbiota and Enzymes
Identification of pesticide impact on the soil microbiome is of the utmost significance today. Diagnosing the response of bacteria to tebuconazole, used for plant protection, may help isolate the most active bacteria applicable in the bioaugmentation of soils contaminated with this preparation. Bear...
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MDPI AG
2022-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/21/7501 |
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| author | Małgorzata Baćmaga Jadwiga Wyszkowska Agata Borowik Jan Kucharski |
| author_facet | Małgorzata Baćmaga Jadwiga Wyszkowska Agata Borowik Jan Kucharski |
| author_sort | Małgorzata Baćmaga |
| collection | DOAJ |
| description | Identification of pesticide impact on the soil microbiome is of the utmost significance today. Diagnosing the response of bacteria to tebuconazole, used for plant protection, may help isolate the most active bacteria applicable in the bioaugmentation of soils contaminated with this preparation. Bearing in mind the above, a study was undertaken to test the effect of tebuconazole on the diversity of bacteria at all taxonomic levels and on the activity of soil enzymes. It was conducted by means of standard and metagenomic methods. Its results showed that tebuconazole applied in doses falling within the ranges of good agricultural practice did not significantly disturb the biological homeostasis of soil and did not diminish its fertility. Tebuconazole was found to stimulate the proliferation of organotrophic bacteria and fungi, and also the activities of soil enzymes responsible for phosphorus, sulfur, and carbon metabolism. It did not impair the activity of urease responsible for urea hydrolysis, or cause any significant changes in the structure of bacterial communities. All analyzed soil samples were mainly populated by bacteria from the phylum <i>Proteobacteria</i>, <i>Actinobacteria</i>, <i>Firmicutes</i>, <i>Gemmatimonadetes</i>, <i>Acidobacteria</i>, <i>Planctomycetes</i>, and <i>Chloroflexi</i>. Bacteria from the genera <i>Kaistobacter</i>, <i>Arthrobacter</i>, and <i>Streptomyces</i> predominated in the soils contaminated with tebuconazole, whereas these from the <i>Gemmata</i> genus were inactivated by this preparation. |
| first_indexed | 2024-03-09T18:48:20Z |
| format | Article |
| id | doaj.art-d245e467e7ba42a687163fef22943611 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:48:20Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d245e467e7ba42a687163fef229436112023-11-24T06:05:16ZengMDPI AGMolecules1420-30492022-11-012721750110.3390/molecules27217501Effects of Tebuconazole Application on Soil Microbiota and EnzymesMałgorzata Baćmaga0Jadwiga Wyszkowska1Agata Borowik2Jan Kucharski3Department of Soil Science and Microbiology, Faculty of Agriculture and Forestry, University of Warmia and Mazury in Olsztyn, Plac Łódzki 3, 10-727 Olsztyn, PolandDepartment of Soil Science and Microbiology, Faculty of Agriculture and Forestry, University of Warmia and Mazury in Olsztyn, Plac Łódzki 3, 10-727 Olsztyn, PolandDepartment of Soil Science and Microbiology, Faculty of Agriculture and Forestry, University of Warmia and Mazury in Olsztyn, Plac Łódzki 3, 10-727 Olsztyn, PolandDepartment of Soil Science and Microbiology, Faculty of Agriculture and Forestry, University of Warmia and Mazury in Olsztyn, Plac Łódzki 3, 10-727 Olsztyn, PolandIdentification of pesticide impact on the soil microbiome is of the utmost significance today. Diagnosing the response of bacteria to tebuconazole, used for plant protection, may help isolate the most active bacteria applicable in the bioaugmentation of soils contaminated with this preparation. Bearing in mind the above, a study was undertaken to test the effect of tebuconazole on the diversity of bacteria at all taxonomic levels and on the activity of soil enzymes. It was conducted by means of standard and metagenomic methods. Its results showed that tebuconazole applied in doses falling within the ranges of good agricultural practice did not significantly disturb the biological homeostasis of soil and did not diminish its fertility. Tebuconazole was found to stimulate the proliferation of organotrophic bacteria and fungi, and also the activities of soil enzymes responsible for phosphorus, sulfur, and carbon metabolism. It did not impair the activity of urease responsible for urea hydrolysis, or cause any significant changes in the structure of bacterial communities. All analyzed soil samples were mainly populated by bacteria from the phylum <i>Proteobacteria</i>, <i>Actinobacteria</i>, <i>Firmicutes</i>, <i>Gemmatimonadetes</i>, <i>Acidobacteria</i>, <i>Planctomycetes</i>, and <i>Chloroflexi</i>. Bacteria from the genera <i>Kaistobacter</i>, <i>Arthrobacter</i>, and <i>Streptomyces</i> predominated in the soils contaminated with tebuconazole, whereas these from the <i>Gemmata</i> genus were inactivated by this preparation.https://www.mdpi.com/1420-3049/27/21/7501tebuconazolesoil microbiomegenetic diversitybiochemical activity of soil |
| spellingShingle | Małgorzata Baćmaga Jadwiga Wyszkowska Agata Borowik Jan Kucharski Effects of Tebuconazole Application on Soil Microbiota and Enzymes Molecules tebuconazole soil microbiome genetic diversity biochemical activity of soil |
| title | Effects of Tebuconazole Application on Soil Microbiota and Enzymes |
| title_full | Effects of Tebuconazole Application on Soil Microbiota and Enzymes |
| title_fullStr | Effects of Tebuconazole Application on Soil Microbiota and Enzymes |
| title_full_unstemmed | Effects of Tebuconazole Application on Soil Microbiota and Enzymes |
| title_short | Effects of Tebuconazole Application on Soil Microbiota and Enzymes |
| title_sort | effects of tebuconazole application on soil microbiota and enzymes |
| topic | tebuconazole soil microbiome genetic diversity biochemical activity of soil |
| url | https://www.mdpi.com/1420-3049/27/21/7501 |
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