HbBIN2 Functions in Plant Cold Stress Resistance through Modulation of HbICE1 Transcriptional Activity and ROS Homeostasis in <i>Hevea brasiliensis</i>
Cold stress poses significant limitations on the growth, latex yield, and ecological distribution of rubber trees (<i>Hevea brasiliensis</i>). The GSK3-like kinase plays a significant role in helping plants adapt to different biotic and abiotic stresses. However, the functions of GSK3-li...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/21/15778 |
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| author | Yi-Min Qiu Jing Guo Wei-Zeng Jiang Jia-Hui Ding Ru-Feng Song Jian-Long Zhang Xi Huang Hong-Mei Yuan |
| author_facet | Yi-Min Qiu Jing Guo Wei-Zeng Jiang Jia-Hui Ding Ru-Feng Song Jian-Long Zhang Xi Huang Hong-Mei Yuan |
| author_sort | Yi-Min Qiu |
| collection | DOAJ |
| description | Cold stress poses significant limitations on the growth, latex yield, and ecological distribution of rubber trees (<i>Hevea brasiliensis</i>). The GSK3-like kinase plays a significant role in helping plants adapt to different biotic and abiotic stresses. However, the functions of GSK3-like kinase BR-INSENSITIVE 2 (BIN2) in <i>Hevea brasiliensis</i> remain elusive. Here, we identified <i>HbBIN2s</i> of <i>Hevea brasiliensis</i> and deciphered their roles in cold stress resistance. The transcript levels of <i>HbBIN2s</i> are upregulated by cold stress. In addition, HbBIN2s are present in both the nucleus and cytoplasm and have the ability to interact with the INDUCER OF CBF EXPRESSION1(HbICE1) transcription factor, a central component in cold signaling. <i>HbBIN2</i> overexpression in <i>Arabidopsis</i> displays decreased tolerance to chilling stress with a lower survival rate and proline content but a higher level of electrolyte leakage (EL) and malondialdehyde (MDA) than wild type under cold stress. Meanwhile, <i>HbBIN2</i> transgenic <i>Arabidopsis</i> treated with cold stress exhibits a significant increase in the accumulation of reactive oxygen species (ROS) and a decrease in the activity of antioxidant enzymes. Further investigation reveals that HbBIN2 inhibits the transcriptional activity of HbICE1, thereby attenuating the expression of <i>C-REPEAT BINDING FACTOR (HbCBF1).</i> Consistent with this, overexpression of <i>HbBIN2</i> represses the expression of CBF pathway cold-regulated genes under cold stress. In conclusion, our findings indicate that HbBIN2 functions as a suppressor of cold stress resistance by modulating HbICE1 transcriptional activity and ROS homeostasis. |
| first_indexed | 2024-03-11T11:29:09Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T11:29:09Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d24a249ff5d94ac1a829e8729dc539592023-11-10T15:05:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124211577810.3390/ijms242115778HbBIN2 Functions in Plant Cold Stress Resistance through Modulation of HbICE1 Transcriptional Activity and ROS Homeostasis in <i>Hevea brasiliensis</i>Yi-Min Qiu0Jing Guo1Wei-Zeng Jiang2Jia-Hui Ding3Ru-Feng Song4Jian-Long Zhang5Xi Huang6Hong-Mei Yuan7School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, ChinaCold stress poses significant limitations on the growth, latex yield, and ecological distribution of rubber trees (<i>Hevea brasiliensis</i>). The GSK3-like kinase plays a significant role in helping plants adapt to different biotic and abiotic stresses. However, the functions of GSK3-like kinase BR-INSENSITIVE 2 (BIN2) in <i>Hevea brasiliensis</i> remain elusive. Here, we identified <i>HbBIN2s</i> of <i>Hevea brasiliensis</i> and deciphered their roles in cold stress resistance. The transcript levels of <i>HbBIN2s</i> are upregulated by cold stress. In addition, HbBIN2s are present in both the nucleus and cytoplasm and have the ability to interact with the INDUCER OF CBF EXPRESSION1(HbICE1) transcription factor, a central component in cold signaling. <i>HbBIN2</i> overexpression in <i>Arabidopsis</i> displays decreased tolerance to chilling stress with a lower survival rate and proline content but a higher level of electrolyte leakage (EL) and malondialdehyde (MDA) than wild type under cold stress. Meanwhile, <i>HbBIN2</i> transgenic <i>Arabidopsis</i> treated with cold stress exhibits a significant increase in the accumulation of reactive oxygen species (ROS) and a decrease in the activity of antioxidant enzymes. Further investigation reveals that HbBIN2 inhibits the transcriptional activity of HbICE1, thereby attenuating the expression of <i>C-REPEAT BINDING FACTOR (HbCBF1).</i> Consistent with this, overexpression of <i>HbBIN2</i> represses the expression of CBF pathway cold-regulated genes under cold stress. In conclusion, our findings indicate that HbBIN2 functions as a suppressor of cold stress resistance by modulating HbICE1 transcriptional activity and ROS homeostasis.https://www.mdpi.com/1422-0067/24/21/15778abiotic stresses<i>Hevea brasiliensis</i>GSK3-like kinaseROStranscriptional regulation |
| spellingShingle | Yi-Min Qiu Jing Guo Wei-Zeng Jiang Jia-Hui Ding Ru-Feng Song Jian-Long Zhang Xi Huang Hong-Mei Yuan HbBIN2 Functions in Plant Cold Stress Resistance through Modulation of HbICE1 Transcriptional Activity and ROS Homeostasis in <i>Hevea brasiliensis</i> International Journal of Molecular Sciences abiotic stresses <i>Hevea brasiliensis</i> GSK3-like kinase ROS transcriptional regulation |
| title | HbBIN2 Functions in Plant Cold Stress Resistance through Modulation of HbICE1 Transcriptional Activity and ROS Homeostasis in <i>Hevea brasiliensis</i> |
| title_full | HbBIN2 Functions in Plant Cold Stress Resistance through Modulation of HbICE1 Transcriptional Activity and ROS Homeostasis in <i>Hevea brasiliensis</i> |
| title_fullStr | HbBIN2 Functions in Plant Cold Stress Resistance through Modulation of HbICE1 Transcriptional Activity and ROS Homeostasis in <i>Hevea brasiliensis</i> |
| title_full_unstemmed | HbBIN2 Functions in Plant Cold Stress Resistance through Modulation of HbICE1 Transcriptional Activity and ROS Homeostasis in <i>Hevea brasiliensis</i> |
| title_short | HbBIN2 Functions in Plant Cold Stress Resistance through Modulation of HbICE1 Transcriptional Activity and ROS Homeostasis in <i>Hevea brasiliensis</i> |
| title_sort | hbbin2 functions in plant cold stress resistance through modulation of hbice1 transcriptional activity and ros homeostasis in i hevea brasiliensis i |
| topic | abiotic stresses <i>Hevea brasiliensis</i> GSK3-like kinase ROS transcriptional regulation |
| url | https://www.mdpi.com/1422-0067/24/21/15778 |
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